Detail
| RLID: | RLID00008369 |
| RNA Symbol: | GAPDH |
| RNA Category: | mRNA |
| Organism: | Homo sapiens |
| Aliase: | G3PD, GAPD, HEL-S-162eP |
| Homology: | Gapdh (Mus musculus), Gapdh (Rattus norvegicus ) |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Mitochondrion | HDF cell | Fluorescence in situ hybridization | 16178658 |
| Description: Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive control studies are performed, including the use of fluorescence in-situ hybridization (FISH), negative-control beacons, and the detection of colocalization of 28S ribosomal RNA with the rough endoplasmic reticulum (ER), suggesting that the mRNA localization and colocalization patterns observed in our study are true and specific. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |
| RLID00008363 | Ribosome | Jurkat cell | Northern blot | 12923260 |
| RLID00008364 | Ribosome | T-cell|SeAx | qRT-PCR | 19175411 |
| RLID00008365 | Ribosome | Colon cancer cell | Next-generation sequencing | 24393600 |
| RLID00008366 | Exosome | Colon|Liver|Lung | qRT-PCR | 22895844 |
| RLID00008367 | Ribosome | T-cell line Jurkat | Northern blot | 12923260 |
| RLID00008368 | Ribosome | T-cell line Jurkat | S1 nuclease protection assays | 12923260 |
| RLID00008370 | Cytosol | HEK293 cell | Northern blot | 21431749 |
| RLID00008371 | Endoplasmic reticulum | HEK293|HeLa|HDF|J558 cell | qRT-PCR | 25142066 |
| RLID00008372 | Cytosol | HeLa cell|HEK293 cell | In situ hybridization | 25142066 |
| RLID00008373 | Ribosome | HEK293|HeLa|HDF|J558 cell | qRT-PCR | 25142066 |
| RLID00008374 | Endoplasmic reticulum | U2OS cell | Fluorescence in situ hybridization | 26272916 |
| RLID00008375 | Ribosome | HEK293 cell | Next-generation sequencing | 22199352 |
| RLID00008376 | Nucleus | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00008377 | Cytoplasm | Nasopharyngeal carcinoma cell | Next-generation sequencing | 20498841 |
| RLID00008378 | Cytoplasm | Embryonic stem cell | qRT-PCR|Microarray | 22193719 |
| RLID00008379 | Cytosol | HT1080 cell | Fluorescence in situ hybridization|qRT-PCR | 25753659 |
| RLID00070362 | chromatin | K562 | CSCD | |
| RLID00070363 | cytosol | HepG2 | CSCD | |
| RLID00070364 | cytosol | K562 | CSCD | |
| RLID00070365 | nucleolus | K562 | CSCD | |
| RLID00070366 | nucleoplasm | K562 | CSCD | |
| RLID00070367 | nucleus | HeLa-S3 | CSCD | |
| RLID00070368 | nucleus | K562 | CSCD | |
| RLID00070369 | exosome | blood | exoRBase |