Detail
Localization |
Tissue |
Validated Method |
PMID |
Endoplasmic reticulum |
U2OS cell |
Fluorescence in situ hybridization |
26272916 |
Description: To determine whether partial ER association was a general phenomenon for all mRNAs, we next investigated the localization of an mRNA encoding a cytosolic protein, glyceraldehyde 3-phosphate dehydrogenase (GAPDH). We could reproducibly find 15% of the GAPDH puncta in digitonin-extracted cells (Fig. 1A,B). However, in contrast to what we had seen for Sec61b and nesprin-2, most of the GAPDH mRNAs were extracted in cells treated with either HHT, or puromycin+EDTA (Fig. 1), suggesting that the small amount of ER association was mediated by translating ribosomes. Data are collected from Figure 1.
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Other Subcellular Localization:
RLID |
Localization |
Tissue |
Validated Method |
PMID |