Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Ribosome |
T-cell|SeAx |
qRT-PCR |
19175411 |
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Description: Using quantitative real-time RT-PCR we evaluated, whether mRNAs coding for differently located proteins are selectively enriched in one of the two analysed compartments, namely free versus membrane-associated polysomes. We selected genes coding for proteins located in the plasma membrane (GPR137B), secreted proteins (TIC2, IBP2, PAI) and cytosolic proteins (the house keeping genes GAPDH and HMBS). In fact, the distribution of the specific mRNA was as predicted ( Fig. 1): The average ratio of specific mRNA at bound ribosomes versus free ribosomes was 1 ? 4.4 for the housekeeping genes (GAPDH and HMBS) and 13.3 ? 1 for genes coding for membrane-bound or secreted proteins. Ratios for genes coding for cytosolic proteins were always below 0.6 and those for membrane or secreted genes were always above 1. The highest values were observed for PAI in MyLa (57 ? 1) and GPR137B in SeAx (34 ? 1), while the lowest ratios were detected for HMBS (1 ? 55) and GAPDH (1 ? 12) in HuT78.
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Other Subcellular Localization:
| RLID |
Localization |
Tissue |
Validated Method |
PMID |