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Description: To examine the effect of copper deficiency on expression of CuZn-SOD genes, reverse transcription PCR (RT-PCR) was performed after culture for 30 d (Fig. 3). Copper deficiency resulted in the repression of two chloroplastic CuZn-SOD genes (PpCSD1 and PpCSD2), but did not affect the expression of two cytosolic CuZn-SOD genes (PpCSD3 and PpCSD4). Expression of the extracellular and chloroplastic Fe-SOD genes (PpFSD1 and PpFSD2) and the mitochondrial Mn-SOD gene (PpMSD) was unaffected by copper deficiency. Because the cytosolic PpFSD3 showed a degenerated gene structure, it seemed likely to be a pseudogene. However, its expression was strongly induced by copper deficiency. This observation will be discussed, along with the copper responsive cis-element, in the Discussion.
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