Detail

Localization	Tissue	Validated Method	PMID
Endoplasmic reticulum	Pollen tube	Fluorescence in situ hybridization	25732863
Description: As germination proceeded, the hybridization and immunolabeling signals were prominent in the cytoplasm of outgrowing pollen tubes (Figs. 2b and 3b). By performing optical sectioning, we observed that PhCRT transcripts accumulated at the base of outgrowing tubes, where they formed rings or collars in the cortical cytoplasm (bracketed region and arrows in Fig. 2b-b' ). In elongated tubes of different lengths, PhCRT mRNA was diffusely distributed throughout the cytoplasm of the tube shank, extending from the base to the subapical zone (Fig. 2c, d). The above results led us to propose that the common sites of PhCRT mRNA, CRT, and 18S rRNA localization in germinating pollen and growing pollen tubes are enriched in ER membrane-bound ribosomes. Together, our results show that the regions in which PhCRT mRNA, CRT protein, and 18S rRNA are localized are also rich in RER.

Other Subcellular Localization:

RLID	Localization	Tissue	Validated Method	PMID
RLID00037400	Cytoplasm	Pollen tube	Fluorescence in situ hybridization	25732863
RLID00037401	Ribosome	Pollen tube	Fluorescence in situ hybridization	25732863