Detail
| RLID: | RLID00037088 |
| RNA Symbol: | U6 |
| RNA Category: | snRNA |
| Organism: | Cricetulus griseus |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Nucleus | Ovary cell | RT-PCR|Northern blot | 16644724 |
| Description: FIGURE 5. XBP1 mRNA splicing occurs in the cytoplasm. CHO/mXBP1ÃŽâ€C(un)-d2EGFP reporter cells were treated with Tm for 4 h and then RNAs were isolated from total (T), nuclear (N), and cytoplasmic (C) fractions and used for RT-PCR analysis (A). To demonstrate the quality of the fractionation, nucleoplasmic (N) and cytoplasmic (C) RNAs were monitored by Northern blot analysis for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (B), tRNALys (C), and U6 small nuclear RNA (D). All RNAs shown were isolated from the same experiment. GADPH is shown as a loading control (E). The image of RNA stained with ethidium bromide in a formaldehyde/agarose gel demonstrates the quality of the RNA. N.B. indicates Northern blot. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |