Detail
| RLID: | RLID00027712 |
| RNA Symbol: | ssc-mir-323 |
| RNA Category: | miRNA |
| Organism: | Sus scrofa |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Exosome | Milk | Next-generation sequencing | 24499489 |
| Description: In this study, exosomes of approximately 100 nm in diameter were isolated from porcine milk through serial centrifugation and ultracentrifugation procedures. Total RNA was extracted from exosomes, and 5S ribosomal RNA was found to be the major RNA component. Solexa sequencing showed a total of 491 miRNAs, including 176 known miRNAs and 315 novel mature miRNAs (representing 366 pre-miRNAs), which were distributed among 30 clusters and 35 families, and two predicted novel miRNAs were verified targeting 3'UTR of IGF-1R by luciferase assay. Interestingly, we observed that three miRNAs (ssc-let-7e, ssc-miR-27a, and ssc-miR-30a) could be generated from miRNA-offset RNAs (moRNAs). Data are collected from Additional file. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |