Detail
| RLID: | RLID00024282 |
| RNA Symbol: | PEX3 |
| RNA Category: | mRNA |
| Organism: | Saccharomyces cerevisiae |
| Aliase: | YDR329C, PAS3 |
| Homology: | PEX3 (Saccharomyces cerevisiae ) |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Cytosol | Yeast | RT-PCR | 23904265 |
| Description: Figure 2: mSMPs cofractionate with ER in WT cells. (A) Fractionation of lysates into ER (microsomal) and cytosolic fractions. WT yeast were lysed and subjected to sucrose density gradient centrifugation as described in Materials and Methods. Aliquots from the different fractions obtained through centrifugation were subjected to SDS–PAGE and detected in blots with antibodies against an ER marker, dolichol phosphate mannose (anti-Dpm1) synthase, or a cytosolic marker, phosphoglycerate kinase (anti-PGK1). Note lack of overlap between markers in the different fractions. (B) Distribution of mSMPs. Total mRNA isolated from the fractions (ER or cytosol [Cyt]) obtained using density gradient centrifugation was subjected to DNase I treatment and RT-PCR with oligonucleotide pairs specific to each mSMP. Genomic DNA served as a positive control for the PCR (Genomic), and RT without RNA template served as a negative control (�. PCR amplification of RNA from the ER and cytosolic fractions without reverse transcription (–RT) served as controls to detect DNA contamination. PCR amplification of RNA from the ER and cytosolic fractions with reverse transcription (+RT) is shown in duplicate. Samples were electrophoresed on agarose gels and documented by ethidium bromide labeling. (C) Distribution of an mRNA encoding a cytosolic protein. Same as in B except that an oligonucleotide pair specific to FSH3 was used. (D) Distribution of mRNAs not encoding mSMPs. Same as in B except that oligonucleotide pairs specific to mRNAs encoding proteins not translocated into the ER, but instead associated either with the cytosolic leaflet of the secretory pathway (SAR1, SEC9, SRO7, YKT6, YPT1) or ribosome (RDN18) were used. Data are collected from Figure 2. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |