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Description: Indeed, in situ RT-PCR and confocal microscopy analysis showed that legumin-1 RNAs are distributed in a pattern markedly distinct from that observed for the zein RNAs. Instead of the punctate pattern indicative of the localization of zein RNAs to the zein PBs, legumin-1 RNAs were distributed as large fluorescent patches. The size and shape of these fluorescent patches and their close proximity to the zein PBs is consistent with the location of legumin-1 RNAs on lamellar membranes of the cis-ER (Fig. 4).
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