Detail
RLID: | RLID00012393 |
RNA Symbol: | nanos1 |
RNA Category: | mRNA |
Organism: | Xenopus laevis |
Aliase: | nos1, xcat-2, xcat2, xnos1 |
Subcellular Localization: |
Localization | Tissue | Validated Method | PMID |
Mitochondrion | Oocyte | In situ hybridization | 8948579 |
Description: In the present study we analyzed the properties of the METRO pathway, which localizes Xlsirt, Xcat2, and Xwnt11 RNAs to a specific region of the vegetal cortex during stage 1 of oogenesis. A combination of methodologies involving both fixed material and living oocytes was used to analyze RNA localization. We show that in early diplotene pre-stage 1 oocytes (25-50 microm in diameter) both endogenous and injected exogenous METRO RNAs translocated to multiple mitochondrial aggregates (pre-mitochondrial clouds) that surround the germinal vesicle (GV). However, by early stage 1 (diplotene oocytes, 50-200 microm), all three of the RNAs discriminated between the different clouds and translocated exclusively within the METRO of a single mitochondrial cloud. |
Other Subcellular Localization:
RLID | Localization | Tissue | Validated Method | PMID |
RLID00012385 | Mitochondrion | Oocyte | In situ hybridization | 7539356 |
RLID00012386 | Vegetal | Oocyte | - | 7867501 |
RLID00012387 | Mitochondrion | Oocyte | - | 7867501 |
RLID00012388 | Germ plasm | Embryo | - | 7867501 |
RLID00012389 | Cytoskeleton | Oocyte | In situ hybridization | 8223259 |
RLID00012390 | Vegetal | Oocyte | In situ hybridization | 8873762 |
RLID00012391 | Germ plasm | Oocyte | In situ hybridization | 8873762 |
RLID00012392 | Cytoplasm | Oocyte | In situ hybridization | 8948579 |
RLID00012394 | Vegetal | Oocyte | In situ hybridization | 8948579 |
RLID00012395 | Vegetal | Oocyte | In situ hybridization | 9739112 |
RLID00012396 | Mitochondrion | Oocyte | In situ hybridization | 9739112 |
RLID00012397 | Mitochondrion | Oocyte | In situ hybridization|Electron microscopy | 10625548 |
RLID00012398 | Vegetal | Oocyte | In situ hybridization | 11309203 |
RLID00012399 | Germ plasm | Oocyte | In situ hybridization | 11784096 |
RLID00012400 | Vegetal | Oocyte | In situ hybridization | 12401170 |
RLID00012401 | Mitochondrion | Oocyte | Immunofluorescence | 15292452 |
RLID00012402 | Mitochondrion | Oocyte | Quantitative RNA localization assay | 19223445 |
RLID00012403 | Germ plasm | Oocyte | Microscopy | 23626739 |
RLID00012404 | Mitochondrion | Oocyte | In situ hybridization | 8787767 |