Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Cell leading edge |
Fibroblast |
Fluorescence in situ hybridization |
15923655 |
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Description: The localization of the Arp2/3 complex mRNAs is dependent on both actin filaments and microtubules, because disruption of either cytoskeletal system (with cytochalasin D and colchicine, respectively) inhibited the localization of all seven subunit mRNAs. To address these questions, double detection of two types of mRNAs simultaneously in the same cells was performed by sequential FISH-TSA. As shown in Fig. 5A-C, Arp3 mRNA appears not to be precisely colocalized with β-actin mRNA, although they both concentrate together in the protrusions. In addition, the Arp2 and Arp3 mRNAs also do not colocalize, although they are both concentrated together in the protrusions (Fig. 5D-F).
|
Other Subcellular Localization:
| RLID |
Localization |
Tissue |
Validated Method |
PMID |
| RLID00011559 |
Cell leading edge |
Fibroblast |
Fluorescence in situ hybridization |
15923655 |