Detail
| Localization |
Tissue |
Validated Method |
PMID |
| Mitochondrion |
Liver |
In situ hybridization |
8726358 |
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Description: A method was developed to study the intracellular distribution of ASS and ASL mRNAs in rat liver at the ultrastructural level, using in situ RT-PCR (reverse transcription and the polymerase chain reaction). The results indicate that these mRNAs are localized to areas of the cytoplasm adjacent to, or in the immediate vicinity of, the mitochondria.For experiments using the ASS primers, ratios similar to those for ASL were obtained, again regardless of the specific experimental procedures followed. For all silver grains, the ratios for mitochondrial outer membrane were 2.36 & 0.40 and 2.18 -+ 0.33, and those for ER were 1.36 * 0.20 and 1.19 2 0.18 after in situ RT-PCR or in situ RT, respectively, while the ratios for mitochondrial matrix and other structures were < 1.0 (Table I, ASS data). Consideration of only medium/large grains gave similar ratios (not shown). Thus, ASS mRNA, like ASL mRNA, is preferentially located at the mitochondrial outer membrane and in the ER.
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Other Subcellular Localization:
| RLID |
Localization |
Tissue |
Validated Method |
PMID |
| RLID00008116 |
Endoplasmic reticulum |
Liver |
In situ hybridization |
8726358 |