Detail

RLID: RLID00004100
RNA Symbol: env
RNA Category: mRNA
Organism: Human immunodeficiency virus 1
Aliase: HIV1gp8
Subcellular Localization:
Localization Tissue Validated Method PMID
Nucleoplasm HeLa cell In situ hybridization 9811683
Description: Using in situ hybridization, we demonstrated that in the absence of Rev, unspliced RNA generated with an HIV-1 env expression construct displayed discrete localization in the nucleus, coincident with the location of the gene and not associated with SC35-containing nuclear speckles. Expression of Rev resulted in a disperse signal for the unspliced RNA throughout both the nucleus and the cytoplasm. Subsequent fractionation of the nucleus revealed that the majority of unspliced viral RNA within the nucleus is associated with the nuclear matrix and that upon expression of Rev, a small proportion of the unspliced RNA is found within the nucleoplasm.

Other Subcellular Localization:

RLID Localization Tissue Validated Method PMID
RLID00004096 Nucleus HeLa cell Confocal microscopy 9811683
RLID00004097 Cytoplasm HeLa cell S1 protection experiments 9811683
RLID00004098 Nucleoplasm HeLa cell S1 protection experiments 9811683
RLID00004099 Nucleus HeLa cell S1 protection experiments 9811683
RLID00004101 Cytoplasm HeLa cell In situ hybridization 9811683
RLID00004102 Nucleus HeLa cell In situ hybridization 9811683
RLID00004103 Nucleus HeLa cell In situ hybridization 9811683