Detail
| RLID: | RLID00004100 |
| RNA Symbol: | env |
| RNA Category: | mRNA |
| Organism: | Human immunodeficiency virus 1 |
| Aliase: | HIV1gp8 |
| Subcellular Localization: | |
| Localization | Tissue | Validated Method | PMID |
| Nucleoplasm | HeLa cell | In situ hybridization | 9811683 |
| Description: Using in situ hybridization, we demonstrated that in the absence of Rev, unspliced RNA generated with an HIV-1 env expression construct displayed discrete localization in the nucleus, coincident with the location of the gene and not associated with SC35-containing nuclear speckles. Expression of Rev resulted in a disperse signal for the unspliced RNA throughout both the nucleus and the cytoplasm. Subsequent fractionation of the nucleus revealed that the majority of unspliced viral RNA within the nucleus is associated with the nuclear matrix and that upon expression of Rev, a small proportion of the unspliced RNA is found within the nucleoplasm. | |||
Other Subcellular Localization:
| RLID | Localization | Tissue | Validated Method | PMID |