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Description: In situ hybridization showed an even distribution of proteolipid mRNA throughout the cytosol in both cell types of the midgut (Fig. 2A), whereas subunit A mRNA was concentrated in the apical regions (Fig. 1A). Proteolipid mRNA distribution paralleled that of rough endoplasmic reticulum, whereas subunit A mRNA distribution paralleled that of free ribosomes: rough endoplasmic reticulum was abundant around the central nucleus but also extended into the entire cytosol, whereas free ribosomes were highly concentrated at the apical portion of the cell (Fig. 5A-C). In Malpighian tubules, however, subunit A mRNA (Fig. 3A) as well as the proteolipid mRNA (not shown) were evenly distributed.
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