The mRNA was detected by transfection assay, western blotting, qRT-PCR, luciferase assay, ChIP, MTT assay, transwell assay in colorectal tumor sample, HCT116 cell, HCT8 cell, LS174T cell, LOVO cell, Caco2 cell, SW480 cell, which can regulate tumor cell growth and motility properties by binding miR-139-5p, freeing mRNAs of RAP1B being repressed by miR-199a*.
The miR-139-5p target genes IGF1R, ROCK2 and RAP1B exert regulatory effects on the miR-139-5p expression level, relying on their ability to compete for miR-139-5p binding. These overexpressed target genes also regulate each others' protein levels through 3'-UTRs, thus regulating tumor cell growth and motility properties.
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