Detail Information

Basic Information:


  VIRBase ID:  

HVID00001854

  Virus:  

Hepacivirus C (Hepatitis C virus subtype 1b,HCV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9980

  Interaction Type:  

Host-Virus interaction

Interactor Information:


Interactor1 Interactor2
Symbol hsa-miR-122-5p NS5B
miRBase
Accession/Entrez ID		 MIMAT0000421 NS5B
Organism Homo sapiens Hepacivirus C (Hepatitis C virus subtype 1b,HCV)
Category miRNA mRNA
Alias - -

ncRNA SNP information:


Resource Symbol SNP ID SNP Position Ref/Alt
miRNASNP-v3 hsa-miR-122-5p
rs1258166649 chr18:58451088    T/C
rs1426570658 chr18:58451093    T/A

RNA Localization:


Resource Symbol Subcellular Localization Tissue or Cell Line PMID
RNALocate hsa-miR-122-5p
Cytoplasm Huh-7 cells|Sorafenib-resistant Huh-7 cells (Huh-7-DR)     29253196
Cytoplasm Huh-7 cells     29486652
Cytoplasm Nasopharyngeal carcinoma cells     20498841
Endoplasmic reticulum Huh-7 cells     26304123
Exosome Breast cancer cell lines (MCF10A|MDA-MB-231)     25621950
Exosome B cell line (Raji)     21505438
Exosome Brain tissue     23382797
Exosome Plasma     23663360
Exosome Human mast cell line (HMC-1)     24009880
Exosome B cell lymphoma cell lines|EBV-transformed lymphoblastoid B cells     25242326
Exosome Blood|Chronic lymphocytic leukemia cells     -
Microvesicle Plasma     23077538
Microvesicle Seminal plasma     23539611
Microvesicle Blood|Fibroblasts|Mesenchymal stem cells|Urine     -
Mitochondrion Cell line (HEK-293|HeLa)     22984580
Nucleus Adjacent noncancerous tissue|HCC tissue|Huh-7 cells|Sorafenib-resistant Huh-7 cells (Huh-7-DR)     29253196
Nucleus Nasopharyngeal carcinoma cells     20498841
Polysome Human cervical cancer cell line (HeLa)     25609084

Interaction Network (The top 100 interactions):


Interactor1: hsa-miR-122-5p
Interactor2: NS5B

Evidence Support:


Strong-Evidence IFA//Luciferase reporter assay//Northern blot//qPCR//qRT-PCR//RPA
Prediction-Evidence ViTa
Support Database ViRBase

References:


PMID 21185047 Target region 5'UTR//NS5B CDS
Source ViRBase Interactor1 expression None
Tissue or cell line Huh7.5 cells Interactor2 expression Downregulation
Description Interestingly, the HCV replicon RNA sequence displayed an additional potential 8 nucleotide seed site for miR-122(MRE 4) found in the coding region of NS5B, the HCV-encoded RNA dependent polymerase (Fig. 1b); miR-122 interactions with this site could potentially explain the remaining signal observed.

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