Detail Information

Basic Information:


  VIRBase ID:  

HVID00001839

  Virus:  

Hepacivirus C (Hepatitis C virus JFH-1,HCV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9923

  Interaction Type:  

Host-Virus interaction

Interactor Information:


Interactor1 Interactor2
Symbol hsa-miR-196a-5p NS5A
miRBase
Accession/Entrez ID		 MIMAT0000226 NS5A
Organism Homo sapiens Hepacivirus C (Hepatitis C virus JFH-1,HCV)
Category miRNA mRNA
Alias - -

ncRNA SNP information:


Resource Symbol SNP ID SNP Position Ref/Alt
miRNASNP-v3 hsa-miR-196a-5p
rs1422312414 chr17:48632534    C/CA
rs190478598 chr17:48632537    C/A
rs372978481 chr17:48632533    C/T
rs751887895 chr17:48632536    A/G
rs931027203 chr17:48632541    A/G
rs931027203 chr17:48632541    A/T
rs1289240119 chr12:53991783    G/A
rs1449318187 chr12:53991762    T/A
rs185070757 chr12:53991774    T/G
rs569910454 chr12:53991777    T/C
rs750731061 chr12:53991771    T/A
rs750731061 chr12:53991771    T/C
rs755062780 chr12:53991778    G/A
rs755062780 chr12:53991778    G/T
rs756640169 chr12:53991773    A/G
rs756640169 chr12:53991773    A/T
rs1167097656 chr17:48632550    C/T
rs767877229 chr12:53991765    G/C

RNA Localization:


Resource Symbol Subcellular Localization Tissue or Cell Line PMID
RNALocate hsa-miR-196a-5p
Cytoplasm Nasopharyngeal carcinoma cells     20498841
Cytoplasm Breast adenocarcinoma cells (MCF-7)|Cervical adenocarcinoma cells (HeLa)     24918059
Exosome Human cervical cancer cell line (HeLa)     25760330
Exosome Serum     18589210
Exosome Hepatocellular carcinoma cell line (HepG2)|Lung epithelial carcinoma cell line (A549)     20615901
Exosome Jurkat-derived T cell line (J77cl20)     21505438
Exosome Brain tissue     23382797
Exosome Colon tissue|Tongue tissue     -
Microvesicle Hepatocellular carcinoma cell line (HepG2)|Lung epithelial carcinoma cell line (A549)     20615901
Microvesicle Hepatoma cells     23771658
Microvesicle Colon cancer cell line (LIM1863)|Fibroblasts|Mesenchymal stem cells|Urine     -
Mitochondrion Skeletal muscle myoblasts     21637849
Mitochondrion Cell line (HEK-293|HeLa)     22984580
Nucleus Breast adenocarcinoma cells (MCF-7)|Cervical adenocarcinoma cells (HeLa)     24918059
Nucleus Nasopharyngeal carcinoma cells     20498841

Related Drug Information:


Resource Symbol Drug Name PubChem
ID		 Function
ncDR hsa-miR-196a-5p
TCS JNK 5a 766949 Drug sensitive
dimethylarsinothious acid, 2,4-pyrimidinediyl ester - Drug resistant
tellurium, trichloro[1,2-hexanedioato(2-)-o,o']-, ammonium - Drug resistant
bisarylpurine - Drug resistant
1-[(4-aminophenyl)-(4-hydroxyphenyl)methylidene]-[3]ferrocenophane - Drug sensitive
Camptothecin Derivative - Drug sensitive
Resource Symbol Drug Name PubChem
ID		 Function Link  
RNAInter hsa-miR-196a-5p
10-Hydroxycamptothecin 97226 Drug interaction    RC00001465
Curcumin 969516 Drug interaction    RC00001473
Eloxatine 5310940 Drug interaction    RC00001475
Imatinib 5291 Drug interaction    RC00001462
Diethylstilbestrol 448537 Drug interaction    RC00001472
Naringin 442428 Drug interaction    RC00001471
Mechlorethamine 4033 Drug interaction    RC00001461
Doxorubicin 31703 Drug interaction    RC00001464
cis-Diaminedichloroplatinum 2767 Drug interaction    RC00001460
Tamoxifen 2733526 Drug interaction    RC00001474
Verapamil 2520 Drug interaction    RC00001459
Sorafenib 216239 Drug interaction    RC00001470
Polylysine 162282 Drug interaction    RC00001469
Arsenic trioxide 14888 Drug interaction    RC00001463
Docetaxel 148124 Drug interaction    RC00001468
Proanthocyanidin 108065 Drug interaction    RC00001467
Fulvestrant 104741 Drug interaction    RC00001466
1,2,6-Tri-O-galloyl-beta-D-glucopyranose - Drug interaction    RC00001476
Trypaflavine - Drug interaction    RC00001477
Resource Symbol Drug Name PubChem ID PMID
NoncoRNA hsa-miR-196a-5p
Gemcitabine 60750       27591936
Cisplatin 5702198       27591936

Interaction Network (The top 100 interactions):


Interactor1: hsa-miR-196a-5p
Interactor2: NS5A

Evidence Support:


Strong-Evidence Luciferase reporter assay//qPCR//qRT-PCR//Western blot
Weak-Evidence Microarray
Prediction-Evidence Targetscan
Support Database ViRBase

References:


PMID 17943132 Target region NS5A CDS
Source ViRBase Interactor1 expression Upregulation
Tissue or cell line Huh7 cells//Huh7.5.1c2 cells//JFH-1 cells Interactor2 expression Downregulation
Description As anticipated, miR-196 was effective against JFH1, but not against J6/JFH, which contained the 'mutant' target site. Similarly, miR-448 only inhibited the replication of JFH1 containing the 'correct' target site, but was ineffective against J6/JFH (Fig. 3b).

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