Basic Information:

  VIRBase ID:  

HHID00105058

  Virus:  

SARS-CoV-2

  Host:  

Homo sapiens

  Confidence Score:  

0.2500

  Interaction Type:  

Host-Host interaction

  Predicted Binding:

 

      hsa-let-7a-5p:  MIMAT0000062          TMPRSS2:  NM_001135099     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hsa-let-7a-5p	TMPRSS2
miRBase Accession/Entrez ID	MIMAT0000062	7113
Organism	Homo sapiens	Homo sapiens
Category	miRNA	mRNA
Alias	-	PRSS10

ncRNA SNP information:

Resource	Symbol	SNP ID	SNP Position	Ref/Alt
miRNASNP-v3	hsa-let-7a-5p	rs1396618771 chr9:94175977    T/C rs201607747 chr9:94175962    T/A rs1176612700 chr11:122146575    C/T rs760725471 chr11:122146573    T/C rs770465876 chr11:122146569    A/G rs751394786 chr22:46112764    T/C rs1362975136 chr11:122146584    A/C

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	TMPRSS2	chr21:42853862(-) A-I      intronic chr21:42853848(-) A-I      intronic chr21:42853888(-) A-I      intronic chr21:42853850(-) A-I      intronic chr21:42853879(-) A-I      intronic chr21:42857482(-) A-I      intronic chr21:42857493(-) A-I      intronic chr21:42857494(-) A-I      intronic chr21:42857532(-) A-I      intronic chr21:42861840(-) A-I      intronic chr21:42863176(-) A-I      intronic chr21:42863181(-) A-I      intronic chr21:42864020(-) A-I      intronic chr21:42857454(-) A-I      intronic chr21:42852821(-) A-I      intronic chr21:42852811(-) A-I      intronic chr21:42868840(-) A-I      intronic chr21:42864037(-) A-I      intronic chr21:42852802(-) A-I      intronic chr21:42852784(-) A-I      intronic chr21:42852783(-) A-I      intronic chr21:42852698(-) A-I      intronic chr21:42868853(-) A-I      intronic chr21:42853773(-) A-I      intronic chr21:42853747(-) A-I      intronic chr21:42852838(-) A-I      intronic chr21:42868879(-) A-I      intronic chr21:42853786(-) A-I      intronic chr21:42853787(-) A-I      intronic chr21:42853866(-) A-I      intronic chr21:42853876(-) A-I      intronic chr21:42853901(-) A-I      intronic chr21:42853902(-) A-I      intronic chr21:42853905(-) A-I      intronic chr21:42853936(-) A-I      intronic chr21:42857436(-) A-I      intronic chr21:42852837(-) A-I      intronic chr21:42852694(-) A-I      intronic chr21:42857467(-) A-I      intronic chr21:42857468(-) A-I      intronic chr21:42857476(-) A-I      intronic chr21:42859902(-) A-I      ncRNA chr21:42859884(-) A-I      ncRNA chr21:42859893(-) A-I      ncRNA chr21:42868835(-) A-I      intronic chr21:42859992(-) A-I      intronic chr21:42855936(-) A-I      intronic chr21:42868944(-) A-I      intronic chr21:42868836(-) A-I      intronic chr21:42852668(-) A-I      intronic chr21:42859993(-) A-I      intronic chr21:42869010(-) A-I      intronic chr21:42863353(-) A-I      intronic chr21:42868900(-) A-I      intronic chr21:42868945(-) A-I      intronic chr21:42852703(-) A-I      intronic chr21:42860827(-) A-I      intronic chr21:42859858(-) A-I      ncRNA chr21:42859831(-) A-I      ncRNA chr21:42859844(-) A-I      ncRNA chr21:42858403(-) A-I      ncRNA

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	TMPRSS2	chr21:42858403(-) A-I intron -    15342557 chr21:42858406(-) A-I intron -    15342557 chr21:42859670(-) A-I intron -    15342557 chr21:42859831(-) A-I intron -    15342557   //15258596 chr21:42859844(-) A-I intron -    15342557   //15258596 chr21:42859858(-) A-I intron -    15342557   //15258596 chr21:42859884(-) A-I intron -    15342557   //15258596 chr21:42859893(-) A-I intron -    15342557   //15258596 chr21:42859902(-) A-I intron -    15342557   //15258596 chr21:42868835(-) A-I intron -    15342557 chr21:42868836(-) A-I intron -    15342557 chr21:42868864(-) A-I intron -    15342557 chr21:42868900(-) A-I intron -    15342557 chr21:42868905(-) A-I intron -    15342557 chr21:42868944(-) A-I intron -    15342557 chr21:42868945(-) A-I intron -    15342557 chr21:42868950(-) A-I intron -    15342557 chr21:42869010(-) A-I intron -    15342557 chr21:42869014(-) A-I intron -    15342557 chr21:42869073(-) A-I intron -    15342557 chr21:42853823(-) A-I intron -    21960545 chr21:42853826(-) A-I intron -    21960545 chr21:42853866(-) A-I intron -    21960545 chr21:42855839(-) A-I intron -    21960545 chr21:42855936(-) A-I intron -    21960545 chr21:42855984(-) A-I intron -    21960545 chr21:42857419(-) A-I intron -    21960545 chr21:42857454(-) A-I intron -    21960545 chr21:42857468(-) A-I intron -    21960545 chr21:42857493(-) A-I intron -    21960545

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	TMPRSS2	chr21:42836773-42836774(-) m6A 3'UTR       25456834

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	hsa-let-7a-5p	Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytoplasm Cell line (HeLa)     21695135 Cytoplasm Breast adenocarcinoma cells (MCF-7)|Cervical adenocarcinoma cells (HeLa)     24918059 Endoplasmic reticulum HEK-293 cells     26304123 Exosome Human milk     28688106 Exosome Glioblastoma cells     19011622 Exosome Hetastatic gastric cancer cell line (AZ-P7a)     20949044 Exosome Breast cancer cell line (MCF-7)     20976003 Exosome Primary dendritic cells|T cell line (J77)     21505438 Exosome Renal cancer cells     21670082 Exosome Serum     22427800 Exosome Brain tissue     23382797 Exosome Plasma     23663360 Exosome Human mast cell line (HMC-1)     24009880 Exosome Breast cancer cell lines (MCF-7|MDA-MB-231)     24468161 Exosome Plasma     24937531 Exosome Human esophageal cancer cell line (EC9706)     25184951 Exosome Blood|B lymphoblastoid cells|Breast milk|Colon tissue|Epithelial cells|Lymphoma tissue|Mast cells|Seminal fluid|Tongue tissue     - Microvesicle Plasma     19002258 Microvesicle Glioblastoma cells     19011622 Microvesicle Plasma     23077538 Microvesicle Seminal plasma     23539611 Microvesicle Hepatoma cells     23771658 Microvesicle Blood|Breast cell line|Colon cancer cell line (LIM1863)|Fibroblasts|Mesenchymal stem cells|Urine     - Mitochondrion Cell line (HEK-293|HeLa)     22984580 Nucleolus Breast adenocarcinoma cells (MCF-7)|Cervical adenocarcinoma cells (HeLa)     24918059 Nucleus Breast adenocarcinoma cells (MCF-7)|Cervical adenocarcinoma cells (HeLa)     24918059 Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus Colon adenocarcinoma cells     20864815 Polysome Human cervical cancer cell line (HeLa)     25609084
	TMPRSS2	Exosome Blood     - Nucleus HCC cell line (HepG2)     - Ribosome Colon cancer cells     24393600

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID	Function
ncDR	hsa-let-7a-5p	Heliangolide 5384466 Drug resistant

Resource	Symbol	Drug Name	PubChem ID	Function	Link
RNAInter	hsa-let-7a-5p	Curcumin 969516 Drug interaction    RC00000193 Formaldehyde 712 Drug interaction    RC00000173 Cytarabine 6253 Drug interaction    RC00000182 Capecitabine 60953 Drug interaction    RC00000189 Gemcitabine 60750 Drug interaction    RC00000188 Prednisone 5865 Drug interaction    RC00000181 Estradiol 5757 Drug interaction    RC00000180 Eloxatine 5310940 Drug interaction    RC00000195 Imatinib 5291 Drug interaction    RC00000179 Cholecalciferol 5280795 Drug interaction    RC00000194 2-{[2-Ethoxy-4-(4-hydroxypiperidin-1-yl)phenyl]amino}-5,11-dimethyl-5,11-dihydro-6H-pyrimido[4,5-b][1,4]benzodiazepin-6-one 46843772 Drug interaction    RC00000199 Tretinoin 444795 Drug interaction    RC00000192 Trichostatin A 444732 Drug interaction    RC00000191 Epirubicin 41867 Drug interaction    RC00000187 Metformin 4091 Drug interaction    RC00000178 Hydroxychloroquine 3652 Drug interaction    RC00000177 Etoposide 36462 Drug interaction    RC00000186 Paclitaxel 36314 Drug interaction    RC00000185 5-Fluorouracil 3385 Drug interaction    RC00000176 Enoxacin 3229 Drug interaction    RC00000175 Doxorubicin 31703 Drug interaction    RC00000184 cis-Diaminedichloroplatinum 2767 Drug interaction    RC00000174 N-(3-(5-Chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluorophenyl)propane-1-sulfonamide 24180719 Drug interaction    RC00000198 Caudatin 21633059 Drug interaction    RC00000197 Polylysine 162282 Drug interaction    RC00000190 Ampligen & InterferonalphaA 16197265 Drug interaction    RC00000196 Arsenic trioxide 14888 Drug interaction    RC00000183 Trypaflavine - Drug interaction    RC00000206 platinum-based doublet chemotherapy - Drug interaction    RC00000200 1,2,6-Tri-O-galloyl-beta-D-glucopyranose - Drug interaction    RC00000201 Aidi injection - Drug interaction    RC00000202 CDF(analogues of curcumin) - Drug interaction    RC00000203 Hydroxamic acid HDACi LAQ824 - Drug interaction    RC00000204 Marine fungal metabolite 1386A - Drug interaction    RC00000205

Resource	Symbol	Drug Name	PubChem ID	PMID
NoncoRNA	hsa-let-7a-5p	Cisplatin 5702198       25356050 Cisplatin 5702198       29565706 Bortezomib 387447       31063487 Doxorubicin 31703       31188543 N-(3-(5-Chloro-1H-pyrrolo[2,3-b]pyridine-3-carbonyl)-2,4-difluorophenyl)propane-1-sulfonamide 24180719       26456124

Interaction Network (The top 100 interactions):

Interactor1: hsa-let-7a-5p
Interactor2: TMPRSS2

Evidence Support:

Prediction-Evidence	microT//miRanda//miRBase//miRDB//miRMap//miRWalk//PicTar//PITA//Targetscan
Support Database	ViRBase

References:

[1]PMID	33428154	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	None	Interactor2 expression	None
Description	To explore the regulatory relationship between the 5 key genes, 3 coronavirus-related genes (ACE2, TMPRSS2, BSG), and their target miRNA, we used 6 miRNA target prediction databases to predict the target miRNA of these genes.

[2]PMID	32992681	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	None	Interactor2 expression	None
Description	Table 2 shows a list of novel potential candidate miRNAs against TMPRSS2 gene which were not studied previously.

[3]PMID	33359190	Target region	3'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	None	Interactor2 expression	None
Description	Albeit, list of putative miRNAs (11 miRNAs) retrieved as result from all the three prediction tools is represented in Fig. 2f.

[4]PMID	32814446	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	None	Interactor2 expression	None
Description	Considering the significance of ACE2 and TMPRSS2 in SARS-CoV-2 infection, we also performed a TargetScan (http://www.targetscan.org/vert_72/) search for the miRNAs that could target these two receptors, and the analysis revealed a list of miRNAs such as, hsa-miRNA 200b-3p, hsa-miRNA 200c-3p and miRNA 429 for ACE2, and hsa-let 7c-5p, hsa-miRNA 98-5p, hsa-let 7f-5p, hsa-let 7a-5p, hsa-let 7g-5p, hsa-let 7b-5p, hsa-miRNA 4458, hsa-let 7e-5p, hsa-let 7i-5p, hsa-let 7d-5p, and hsa-miRNA 4500 for TMPRSS2.