Basic Information:

  VIRBase ID:  

HHID00026639

  Virus:  

Hepatitis B virus (HBV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9996

  Interaction Type:  

Host-Host interaction

  Predicted Binding:

 

      hsa-miR-122-5p:  MIMAT0000421          CCNG1:  NR_157055     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hsa-miR-122-5p	CCNG1
miRBase Accession/Entrez ID	MIMAT0000421	900
Organism	Homo sapiens	Homo sapiens
Category	miRNA	mRNA
Alias	-	CCNG

ncRNA SNP information:

Resource	Symbol	SNP ID	SNP Position	Ref/Alt
miRNASNP-v3	hsa-miR-122-5p	rs1258166649 chr18:58451088    T/C rs1426570658 chr18:58451093    T/A

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	CCNG1	chr5:162873961(+) A-I      3'UTR chr5:162873874(+) A-I      3'UTR chr5:162873835(+) A-I      3'UTR chr5:162873787(+) A-I      3'UTR chr5:162865794(+) A-I      intronic chr5:162875046(+) A-I      3'UTR chr5:162873759(+) A-I      3'UTR chr5:162873927(+) A-I      3'UTR chr5:162874256(+) A-I      3'UTR chr5:162873766(+) A-I      3'UTR chr5:162875073(+) A-I      3'UTR chr5:162873801(+) A-I      3'UTR chr5:162874367(+) A-I      3'UTR chr5:162873915(+) A-I      3'UTR chr5:162874420(+) A-I      3'UTR

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	CCNG1	chr5:162865794(+) A-G intron -    22484847 chr5:162865937(+) C-T intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	CCNG1	chr5:162864671-162864672(+) m6A 5'UTR//exon//intron       24981863 chr5:162870699-162870700(+) m6A 3'UTR//CDS//exon       24981863//22608085 chr5:162870779-162870780(+) m6A 3'UTR//exon       24981863//26404942//22608085 chr5:162870808-162870809(+) m6A 3'UTR//exon       24981863//26404942//22608085 chr5:162870818-162870819(+) m6A 3'UTR//exon       24981863//26404942//22608085 chr5:162871185-162871186(+) m6A 3'UTR//intron       24981863//27773535 chr5:162871227-162871228(+) m6A 3'UTR//intron       24981863//27773535 chr5:162871290-162871291(+) m6A 3'UTR//intron       24981863 chr5:162871467-162871468(+) m6A 3'UTR//exon       24981863 chr5:162871497-162871498(+) m6A 3'UTR//exon//intron       24981863 chr5:162871507-162871508(+) m6A 3'UTR//exon//intron       24981863 chr5:162871531-162871532(+) m6A 3'UTR//exon//intron       24981863 chr5:162871607-162871608(+) m6A 3'UTR//exon//intron       26404942 chr5:162871827-162871828(+) m6A 3'UTR//exon//intron       27773535 chr5:162871859-162871860(+) m6A 3'UTR//exon//intron       27773535 chr5:162871955-162871956(+) m6A 3'UTR//exon//intron       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	hsa-miR-122-5p	Cytoplasm Huh-7 cells|Sorafenib-resistant Huh-7 cells (Huh-7-DR)     29253196 Cytoplasm Huh-7 cells     29486652 Cytoplasm Nasopharyngeal carcinoma cells     20498841 Endoplasmic reticulum Huh-7 cells     26304123 Exosome Breast cancer cell lines (MCF10A|MDA-MB-231)     25621950 Exosome B cell line (Raji)     21505438 Exosome Brain tissue     23382797 Exosome Plasma     23663360 Exosome Human mast cell line (HMC-1)     24009880 Exosome B cell lymphoma cell lines|EBV-transformed lymphoblastoid B cells     25242326 Exosome Blood|Chronic lymphocytic leukemia cells     - Microvesicle Plasma     23077538 Microvesicle Seminal plasma     23539611 Microvesicle Blood|Fibroblasts|Mesenchymal stem cells|Urine     - Mitochondrion Cell line (HEK-293|HeLa)     22984580 Nucleus Adjacent noncancerous tissue|HCC tissue|Huh-7 cells|Sorafenib-resistant Huh-7 cells (Huh-7-DR)     29253196 Nucleus Nasopharyngeal carcinoma cells     20498841 Polysome Human cervical cancer cell line (HeLa)     25609084
	CCNG1	Chromatin K562 cells     - Cytosol HCC cell line (HepG2)     - Exosome Blood     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|K562 cells     - Ribosome-free cytosol Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: hsa-miR-122-5p
Interactor2: CCNG1

Evidence Support:

Strong-Evidence	ISH//Luciferase reporter assay//Northern blot//qPCR//RT-PCR//RT-qPCR//Western blot
Weak-Evidence	Microarray
Prediction-Evidence	Targetscan
Support Database	ViRBase//VIRmiRNA

References:

[1]PMID	31366735	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	Huh7 cells	Interactor2 expression	None
Description	In addition, our findings suggest that ADAR1 adopts the cyclin G1 and p53 proteins as downstream molecules of miR122 to reduce the HBV RNA levels. As expected, the ADAR1- and miR-122 mimic transfectants showed decreased cyclin G1 expression with concomitant up-regulation of the p53 protein (Fig. 6A).

[2]PMID	22105316	Target region	3'UTR
Source	ViRBase	Interactor1 expression	Downregulation
Tissue or cell line	HCT116 cells//HepG2 cells//Huh7 cells	Interactor2 expression	Upregulation
Description	We also observed that cyclin G(1) expression was up-regulated in HBV-infected patients, and cyclin G(1) levels were inversely associated with miR-122 expression in liver tissues.

[3]PMID	21565290	Target region	3'UTR
Source	VIRmiRNA	Interactor1 expression	None
Tissue or cell line	Hepatoma cells	Interactor2 expression	None
Description	We listed the cellular functions of these relevant miRNAs in liver disease and their biological properties associated with HBV or HBV-related HCC in Table 1. We also summarized the known changes of miRNAs involved in multiple steps of chronic hepatitis B, from hepatitis to cirrhosis and finally to HCC (Fig. 3).