Basic Information:

  VIRBase ID:  

HHID00019513

  Virus:  

Hepacivirus C (Hepatitis C virus subtype 2a,HCV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9526

  Interaction Type:  

Host-Host interaction

  Predicted Binding:

 

      Predicted binding sites in PRIdictor:   HSP90B1:  NP_003290     

Interactor Information:

	Interactor1	Interactor2
Symbol	hsa-miR-122-5p	HSP90B1
miRBase Accession/Entrez ID	MIMAT0000421	7184
Organism	Homo sapiens	Homo sapiens
Category	miRNA	protein
Alias	-	ECGP//GP96//GRP94//HEL-S-125m//HEL35//TRA1

ncRNA SNP information:

Resource	Symbol	SNP ID	SNP Position	Ref/Alt
miRNASNP-v3	hsa-miR-122-5p	rs1258166649 chr18:58451088    T/C rs1426570658 chr18:58451093    T/A

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	HSP90B1	chr12:104331716(+) A-I      intronic chr12:104328741(+) A-I      intronic chr12:104330314(+) A-I      intronic chr12:104330165(+) A-I      intronic chr12:104328605(+) A-I      intronic chr12:104338434(+) A-I      intronic chr12:104331735(+) A-I      intronic chr12:104339660(+) A-I      intronic chr12:104328584(+) A-I      intronic chr12:104328579(+) A-I      intronic chr12:104328560(+) A-I      intronic chr12:104328557(+) A-I      intronic chr12:104330304(+) A-I      intronic chr12:104328484(+) A-I      intronic chr12:104330236(+) A-I      intronic chr12:104330345(+) A-I      intronic chr12:104331789(+) A-I      intronic chr12:104331869(+) A-I      intronic chr12:104331898(+) A-I      intronic chr12:104330288(+) A-I      intronic chr12:104330170(+) A-I      intronic chr12:104328641(+) A-I      intronic chr12:104328606(+) A-I      intronic chr12:104331815(+) A-I      intronic chr12:104339008(+) A-I      intronic chr12:104330162(+) A-I      intronic chr12:104331838(+) A-I      intronic chr12:104339680(+) A-I      intronic chr12:104331828(+) A-I      intronic chr12:104330366(+) A-I      intronic chr12:104339653(+) A-I      intronic chr12:104330278(+) A-I      intronic chr12:104332947(+) A-I      intronic chr12:104331890(+) A-I      intronic chr12:104328480(+) A-I      intronic chr12:104339044(+) A-I      intronic chr12:104330142(+) A-I      intronic chr12:104331845(+) A-I      intronic chr12:104331769(+) A-I      intronic chr12:104328753(+) A-I      intronic chr12:104331690(+) A-I      intronic chr12:104338358(+) A-I      intronic chr12:104330270(+) A-I      intronic chr12:104328756(+) A-I      intronic chr12:104339100(+) A-I      intronic chr12:104330210(+) A-I      intronic chr12:104328580(+) A-I      intronic chr12:104338997(+) A-I      intronic chr12:104339607(+) A-I      intronic chr12:104330200(+) A-I      intronic chr12:104330238(+) A-I      intronic chr12:104330830(+) A-I      intronic chr12:104328662(+) A-I      intronic chr12:104339064(+) A-I      intronic chr12:104338438(+) A-I      intronic chr12:104330318(+) A-I      intronic chr12:104331933(+) A-I      intronic chr12:104330187(+) A-I      intronic chr12:104331681(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	HSP90B1	chr12:104330210(+) A-I intron -    22327324 chr12:104330304(+) A-I intron -    22327324 chr12:104330318(+) A-I intron -    22327324 chr12:104330162(+) A-I intron -    21960545 chr12:104330996(+) A-I intron -    21960545 chr12:104330162(+) A-G intron -    22484847 chr12:104330200(+) A-G intron -    22484847 chr12:104330210(+) A-G intron -    22484847 chr12:104330278(+) A-G intron -    22484847 chr12:104330304(+) A-G intron -    22484847 chr12:104338358(+) A-G intron -    22484847 chr12:104339008(+) A-G intron -    22484847 chr12:104339695(+) C-A intron -    22484847 chr12:104339698(+) T-A intron -    22484847 chr12:104328580(+) A-G intron -    22484847 chr12:104328662(+) A-G intron -    22484847 chr12:104331735(+) A-G intron -    22484847 chr12:104332890(+) C-T intron -    22484847 chr12:104338523(+) G-A intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	HSP90B1	chr12:104324196-104324197(+) m6A 5'UTR//exon//intron       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr12:104324225-104324226(+) m6A 5'UTR//exon//intron       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr12:104324268-104324269(+) m6A 5'UTR//exon//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr12:104324275-104324276(+) m6A 5'UTR//exon//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr12:104325398-104325399(+) Gm 5'UTR//CDS//exon       - chr12:104325417-104325418(+) m6A exon//intron       24209618//24981863//27773535//22575960 chr12:104325444-104325445(+) m6A exon//intron       24284625//24209618//24981863//27773535//22575960 chr12:104326062-104326063(+) m1A 3'UTR//5'UTR//CDS//exon       26863196 chr12:104326105-104326106(+) m6A 3'UTR//5'UTR//CDS//exon       24981863//27773535//22575960 chr12:104326162-104326163(+) m6A 3'UTR//CDS//exon       24981863//27773535//22575960 chr12:104326617-104326618(+) m6A 3'UTR//CDS//exon       24981863//26404942//27773535//22575960 chr12:104326701-104326702(+) m6A 3'UTR//CDS//exon       24981863//26404942//27773535//22575960 chr12:104327749-104327750(+) m6A 3'UTR//CDS//exon       24981863//26404942//27773535//22575960 chr12:104327767-104327768(+) m6A 3'UTR//CDS//exon       24981863//26404942//27773535//22575960 chr12:104327806-104327807(+) m6A CDS//exon       24981863//26404942//27773535 chr12:104327832-104327833(+) m6A CDS//exon       24981863//26404942//27773535 chr12:104327848-104327849(+) m6A CDS//exon       24981863//26404942//27773535 chr12:104327963-104327964(+) m6A CDS//exon       27773535 chr12:104328037-104328038(+) m6A CDS//exon       26404942//27773535 chr12:104331555-104331556(+) m6A CDS//exon       24981863//26404942//27773535 chr12:104332021-104332022(+) m6A exon//intron       24981863//27773535//22608085 chr12:104332061-104332062(+) m6A exon//intron       24981863//27773535//22575960//22608085 chr12:104332080-104332081(+) m6A exon//intron       24981863//27773535//22575960//22608085 chr12:104332117-104332118(+) m6A CDS//exon       24981863//27773535//22575960//22608085 chr12:104332123-104332124(+) m6A CDS//exon       24981863//27773535//22575960//22608085 chr12:104332221-104332222(+) m6A CDS//exon//intron       24981863//27773535//22575960//22608085 chr12:104332228-104332229(+) m6A CDS//exon//intron       24981863//27773535//22575960//22608085 chr12:104333295-104333296(+) m6A CDS//exon//intron       24981863//27773535//22575960//22608085 chr12:104333305-104333306(+) m6A CDS//exon//intron       24981863//27773535//22575960//22608085 chr12:104333312-104333313(+) m6A CDS//exon//intron       24981863//27773535//22575960//22608085 chr12:104333330-104333331(+) m6A CDS//exon//intron       24981863//27773535//22575960//22608085 chr12:104333345-104333346(+) m6A CDS//exon//intron       24981863//27773535//22608085 chr12:104335297-104335298(+) Tm CDS//exon//intron       - chr12:104335613-104335614(+) m6A CDS//exon//intron       27773535 chr12:104335648-104335649(+) m6A CDS//exon//intron       27773535//22575960 chr12:104335665-104335666(+) m6A CDS//exon//intron       27773535//22575960 chr12:104336338-104336339(+) m6A CDS//exon//intron       27773535//22575960 chr12:104336425-104336426(+) m6A CDS//exon//intron       27773535//22575960 chr12:104336439-104336440(+) m6A CDS//exon//intron       27773535//22575960 chr12:104336453-104336454(+) m6A CDS//exon//intron       27773535//22575960 chr12:104336503-104336504(+) m6A CDS//exon//intron       27773535//22575960 chr12:104336531-104336532(+) m6A CDS//exon//intron       27773535 chr12:104336536-104336537(+) m6A CDS//exon//intron       27773535 chr12:104336583-104336584(+) m6A exon//intron       27773535 chr12:104336658-104336659(+) m6A exon//intron       27773535 chr12:104336916-104336917(+) m6A CDS//exon//intron       24981863//27773535//22575960 chr12:104337016-104337017(+) m6A CDS//exon//intron       24981863//27773535//22575960 chr12:104337092-104337093(+) m6A CDS//exon//intron       24981863//27773535//22575960 chr12:104337162-104337163(+) m6A exon//intron       27773535//22575960 chr12:104337639-104337640(+) m6A CDS//exon//intron       24981863//27773535 chr12:104340417-104340418(+) m6A CDS//exon//intron       24981863//27773535 chr12:104340437-104340438(+) m6A CDS//exon//intron       24981863//27773535 chr12:104340454-104340455(+) m6A CDS//exon//intron       24981863//27773535 chr12:104340581-104340582(+) m6A CDS//exon//intron       24981863//27773535 chr12:104340614-104340615(+) m6A CDS//exon//intron       24981863//27773535 chr12:104340648-104340649(+) m6A CDS//exon//intron       24981863//27773535 chr12:104340699-104340700(+) m6A CDS//exon//intron       25456834//24981863//28052920//27773535//27371828//22575960 chr12:104341095-104341096(+) m1A CDS//exon//intron       26863196 chr12:104341107-104341108(+) m1A CDS//exon//intron       26863196 chr12:104341111-104341112(+) m6A CDS//exon//intron       25456834//24981863//28052920//27773535//27371828//22575960 chr12:104341118-104341119(+) Gm CDS//exon//intron       - chr12:104341121-104341122(+) m6A CDS//exon//intron       25456834//24981863//28052920//27773535//27371828//22575960 chr12:104341131-104341132(+) m6A CDS//exon//intron       25456834//24981863//28052920//27773535//27371828//22575960 chr12:104341143-104341144(+) m6A CDS//exon//intron       25456834//24981863//28052920//27773535//27371828//22575960 chr12:104341181-104341182(+) m6A 3'UTR//CDS//exon       25456834//24981863//28052920//27773535//27371828//22575960 chr12:104341191-104341192(+) m1A 3'UTR//CDS//exon       26863196 chr12:104341199-104341200(+) m6A 3'UTR//CDS//exon       25456834//24981863//28052920//27773535//27371828//22575960 chr12:104341511-104341512(+) m6A 3'UTR//exon//intron       24981863//26404942//27773535//22575960 chr12:104347177-104347178(+) m6A 3'UTR       27773535 chr12:104347270-104347271(+) m6A 3'UTR       27773535//27773536 chr12:104347294-104347295(+) m6A 3'UTR       27773535//27773536 chr12:104347321-104347322(+) m6A 3'UTR       27773535//27773536 chr12:104347362-104347363(+) m6A 3'UTR       27773535 chr12:104347370-104347371(+) m6A 3'UTR       27773535 chr12:104347383-104347384(+) m6A 3'UTR       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	hsa-miR-122-5p	Cytoplasm Huh-7 cells|Sorafenib-resistant Huh-7 cells (Huh-7-DR)     29253196 Cytoplasm Huh-7 cells     29486652 Cytoplasm Nasopharyngeal carcinoma cells     20498841 Endoplasmic reticulum Huh-7 cells     26304123 Exosome Breast cancer cell lines (MCF10A|MDA-MB-231)     25621950 Exosome B cell line (Raji)     21505438 Exosome Brain tissue     23382797 Exosome Plasma     23663360 Exosome Human mast cell line (HMC-1)     24009880 Exosome B cell lymphoma cell lines|EBV-transformed lymphoblastoid B cells     25242326 Exosome Blood|Chronic lymphocytic leukemia cells     - Microvesicle Plasma     23077538 Microvesicle Seminal plasma     23539611 Microvesicle Blood|Fibroblasts|Mesenchymal stem cells|Urine     - Mitochondrion Cell line (HEK-293|HeLa)     22984580 Nucleus Adjacent noncancerous tissue|HCC tissue|Huh-7 cells|Sorafenib-resistant Huh-7 cells (Huh-7-DR)     29253196 Nucleus Nasopharyngeal carcinoma cells     20498841 Polysome Human cervical cancer cell line (HeLa)     25609084
	HSP90B1	Chromatin K562 cells     - Cytosol K562 cells     - Cytosolic polysome Human T lymphocytes (Jurkat E6-1)     12923260 Endoplasmic reticulum HEK-293T cells     21431749 Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539 ER-bound polysome Human T lymphocytes (Jurkat E6-1)     12923260 Exosome Blood     - Membrane-bound ribosome Cell line (HeLa)     18192611 Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus Colon cancer cells     24393600 Nucleus HCC cell line (HepG2)|K562 cells     - Perinuclear Human embryonic kidney cell line (HEK-293)     12923260 Ribosome HEK-293 cells     22199352 Ribosome HeLa cells     25063809

Interaction Network (The top 100 interactions):

Interactor1: hsa-miR-122-5p
Interactor2: HSP90B1

Evidence Support:

Strong-Evidence	qRT-PCR//TaqMan miRNA assay//Western blot
Support Database	ViRBase

References:

PMID	22898980	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	Huh7.5 cells	Interactor2 expression	None
Description	Overexpression of GW182 significantly increased HCV RNA and protein expression in HCV J6/JFH1 infected Huh7.5 cells. Furthermore, GW182 colocalized and coimmunoprecipitated with heat shock protein 90 (HSP90), which increased upon alcohol exposure with and without HCV infection and enhanced HCV gene expression. The use of an HSP90 inhibitor or knockdown of HSP90 decreased GW182 and miR-122 expression and significantly reduced HCV replication.