Basic Information:

  VIRBase ID:  

HHID00019074

  Virus:  

Hepacivirus C (HCV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9526

  Interaction Type:  

Host-Host interaction

  Predicted Binding:

 

      Predicted binding sites in PRIdictor:   AGO2:  NP_036286     

Interactor Information:

	Interactor1	Interactor2
Symbol	hsa-miR-122-5p	AGO2
miRBase Accession/Entrez ID	MIMAT0000421	27161
Organism	Homo sapiens	Homo sapiens
Category	miRNA	protein
Alias	-	CASC7//EIF2C2//LESKRES//LINC00980//PPD//Q10

ncRNA SNP information:

Resource	Symbol	SNP ID	SNP Position	Ref/Alt
miRNASNP-v3	hsa-miR-122-5p	rs1258166649 chr18:58451088    T/C rs1426570658 chr18:58451093    T/A

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	AGO2	chr8:141615468(-) A-I      intronic chr8:141615415(-) A-I      intronic chr8:141615363(-) A-I      intronic chr8:141628901(-) A-I      intronic chr8:141614864(-) A-I      intronic chr8:141614863(-) A-I      intronic chr8:141614802(-) A-I      intronic chr8:141614761(-) A-I      intronic chr8:141614663(-) A-I      intronic chr8:141610753(-) A-I      intronic chr8:141610703(-) A-I      intronic chr8:141610696(-) A-I      intronic chr8:141581965(-) A-I      intronic chr8:141610679(-) A-I      intronic chr8:141628158(-) A-I      intronic chr8:141628069(-) A-I      intronic chr8:141627960(-) A-I      intronic chr8:141627953(-) A-I      intronic chr8:141641025(-) A-I      intronic chr8:141638719(-) A-I      intronic chr8:141638686(-) A-I      intronic chr8:141630927(-) A-I      intronic chr8:141630870(-) A-I      intronic chr8:141628832(-) A-I      intronic chr8:141628450(-) A-I      intronic chr8:141628417(-) A-I      intronic chr8:141627948(-) A-I      intronic chr8:141627924(-) A-I      intronic chr8:141627624(-) A-I      intronic chr8:141627579(-) A-I      intronic chr8:141601075(-) A-I      intronic chr8:141563312(-) A-I      intronic chr8:141621699(-) A-I      intronic chr8:141621722(-) A-I      intronic chr8:141622622(-) A-I      intronic chr8:141573390(-) A-I      intronic chr8:141565531(-) A-I      intronic chr8:141565480(-) A-I      intronic chr8:141565464(-) A-I      intronic chr8:141565002(-) A-I      intronic chr8:141564959(-) A-I      intronic chr8:141622751(-) A-I      intronic chr8:141623199(-) A-I      intronic chr8:141563576(-) A-I      intronic chr8:141623252(-) A-I      intronic chr8:141624241(-) A-I      intronic chr8:141624261(-) A-I      intronic chr8:141624267(-) A-I      intronic chr8:141624340(-) A-I      intronic chr8:141624409(-) A-I      intronic chr8:141624415(-) A-I      intronic chr8:141627387(-) A-I      intronic chr8:141627418(-) A-I      intronic chr8:141627448(-) A-I      intronic chr8:141627483(-) A-I      intronic chr8:141627534(-) A-I      intronic chr8:141627553(-) A-I      intronic chr8:141610631(-) A-I      intronic chr8:141610597(-) A-I      intronic chr8:141610588(-) A-I      intronic chr8:141610521(-) A-I      intronic chr8:141610040(-) A-I      intronic chr8:141610009(-) A-I      intronic chr8:141609932(-) A-I      intronic chr8:141609918(-) A-I      intronic chr8:141606587(-) A-I      intronic chr8:141606438(-) A-I      intronic chr8:141606437(-) A-I      intronic chr8:141606426(-) A-I      intronic chr8:141606198(-) A-I      intronic chr8:141606194(-) A-I      intronic chr8:141604824(-) A-I      intronic chr8:141604784(-) A-I      intronic chr8:141604690(-) A-I      intronic chr8:141604670(-) A-I      intronic chr8:141604553(-) A-I      intronic chr8:141627555(-) A-I      intronic chr8:141627570(-) A-I      intronic chr8:141627571(-) A-I      intronic chr8:141547674(-) A-I      intronic chr8:141609985(-) A-I      intronic chr8:141609990(-) A-I      intronic chr8:141609998(-) A-I      intronic chr8:141628271(-) A-I      intronic chr8:141628270(-) A-I      intronic chr8:141621631(-) A-I      intronic chr8:141621630(-) A-I      intronic chr8:141621594(-) A-I      intronic chr8:141620534(-) A-I      intronic chr8:141620506(-) A-I      intronic chr8:141547652(-) A-I      intronic chr8:141547651(-) A-I      intronic chr8:141614869(-) A-I      intronic chr8:141603484(-) A-I      intronic chr8:141603428(-) A-I      intronic chr8:141603419(-) A-I      intronic chr8:141602119(-) A-I      intronic chr8:141591556(-) A-I      intronic chr8:141591555(-) A-I      intronic chr8:141591549(-) A-I      intronic chr8:141547647(-) A-I      intronic chr8:141620473(-) A-I      intronic chr8:141620449(-) A-I      intronic chr8:141620445(-) A-I      intronic chr8:141584445(-) A-I      intronic chr8:141620396(-) A-I      intronic chr8:141549090(-) A-I      intronic chr8:141551919(-) A-I      intronic chr8:141609991(-) A-I      intronic chr8:141610039(-) A-I      intronic chr8:141620192(-) A-I      intronic chr8:141621512(-) A-I      intronic chr8:141623177(-) A-I      intronic chr8:141628336(-) A-I      intronic chr8:141628827(-) A-I      intronic chr8:141643244(-) A-I      intronic chr8:141641036(-) A-I      intronic chr8:141620392(-) A-I      intronic chr8:141620297(-) A-I      intronic chr8:141546511(-) A-I      intronic chr8:141546537(-) A-I      intronic chr8:141546593(-) A-I      intronic chr8:141546594(-) A-I      intronic chr8:141546599(-) A-I      intronic chr8:141548997(-) A-I      intronic chr8:141549017(-) A-I      intronic chr8:141549080(-) A-I      intronic chr8:141549102(-) A-I      intronic chr8:141553114(-) A-I      intronic chr8:141553149(-) A-I      intronic chr8:141615469(-) A-I      intronic chr8:141553746(-) A-I      intronic chr8:141622667(-) A-I      intronic chr8:141565504(-) A-I      intronic chr8:141563361(-) A-I      intronic chr8:141563431(-) A-I      intronic chr8:141628761(-) A-I      intronic chr8:141628093(-) A-I      intronic chr8:141610523(-) A-I      intronic chr8:141610110(-) A-I      intronic chr8:141641053(-) A-I      intronic chr8:141553719(-) A-I      intronic chr8:141549201(-) A-I      intronic chr8:141627522(-) A-I      intronic chr8:141603469(-) A-I      intronic chr8:141643140(-) A-I      intronic chr8:141638660(-) A-I      intronic chr8:141627449(-) A-I      intronic chr8:141641475(-) A-I      intronic chr8:141553173(-) A-I      intronic chr8:141548956(-) A-I      intronic chr8:141553074(-) A-I      intronic chr8:141551939(-) A-I      intronic chr8:141606570(-) A-I      intronic chr8:141576053(-) A-I      intronic chr8:141549140(-) A-I      intronic chr8:141638833(-) A-I      intronic chr8:141614758(-) A-I      intronic chr8:141622694(-) A-I      intronic chr8:141620101(-) A-I      intronic chr8:141603470(-) A-I      intronic chr8:141549032(-) A-I      intronic chr8:141628478(-) A-I      intronic chr8:141614767(-) A-I      intronic chr8:141606554(-) A-I      intronic chr8:141551874(-) A-I      intronic chr8:141627913(-) A-I      intronic chr8:141549150(-) A-I      intronic chr8:141628337(-) A-I      intronic chr8:141610048(-) A-I      intronic chr8:141564074(-) A-I      intronic chr8:141606221(-) A-I      intronic chr8:141627641(-) A-I      intronic chr8:141639011(-) A-I      intronic chr8:141603580(-) A-I      intronic chr8:141546590(-) A-I      intronic chr8:141565023(-) A-I      intronic chr8:141553654(-) A-I      intronic chr8:141563836(-) A-I      intronic chr8:141553154(-) A-I      intronic chr8:141620454(-) A-I      intronic chr8:141609981(-) A-I      intronic chr8:141641484(-) A-I      intronic chr8:141580427(-) A-I      intronic chr8:141620455(-) A-I      intronic chr8:141563647(-) A-I      intronic chr8:141610112(-) A-I      intronic chr8:141547627(-) A-I      intronic chr8:141643079(-) A-I      intronic chr8:141563297(-) A-I      intronic chr8:141553679(-) A-I      intronic chr8:141621605(-) A-I      intronic chr8:141620182(-) A-I      intronic chr8:141614850(-) A-I      intronic chr8:141615369(-) A-I      intronic chr8:141624354(-) A-I      intronic chr8:141549200(-) A-I      intronic chr8:141630908(-) A-I      intronic chr8:141575986(-) A-I      intronic chr8:141622788(-) A-I      intronic chr8:141575936(-) A-I      intronic chr8:141622639(-) A-I      intronic chr8:141575063(-) A-I      intronic chr8:141631075(-) A-I      intronic chr8:141553722(-) A-I      intronic chr8:141604643(-) A-I      intronic chr8:141546460(-) A-I      intronic chr8:141621606(-) A-I      intronic chr8:141543823(-) A-I      intronic chr8:141563423(-) A-I      intronic chr8:141643238(-) A-I      intronic chr8:141628466(-) A-I      intronic chr8:141549020(-) A-I      intronic chr8:141638683(-) A-I      intronic chr8:141553108(-) A-I      intronic chr8:141627625(-) A-I      intronic chr8:141615358(-) A-I      intronic chr8:141624177(-) A-I      intronic chr8:141606220(-) A-I      intronic chr8:141624236(-) A-I      intronic chr8:141627944(-) A-I      intronic chr8:141549107(-) A-I      intronic chr8:141563936(-) A-I      intronic chr8:141549028(-) A-I      intronic chr8:141547668(-) A-I      intronic chr8:141606600(-) A-I      intronic chr8:141630916(-) A-I      intronic chr8:141615451(-) A-I      intronic chr8:141622621(-) A-I      intronic chr8:141603541(-) A-I      intronic chr8:141625226(-) A-I      intronic chr8:141620252(-) A-I      intronic chr8:141546558(-) A-I      intronic chr8:141622699(-) A-I      intronic chr8:141615366(-) A-I      intronic chr8:141604644(-) A-I      intronic chr8:141615259(-) A-I      intronic chr8:141565571(-) A-I      intronic chr8:141622768(-) A-I      intronic chr8:141553693(-) A-I      intronic chr8:141621565(-) A-I      intronic chr8:141582037(-) A-I      intronic chr8:141606643(-) A-I      intronic chr8:141623077(-) A-I      intronic chr8:141548943(-) A-I      intronic chr8:141563909(-) A-I      intronic chr8:141622620(-) A-I      intronic chr8:141548982(-) A-I      intronic chr8:141547619(-) A-I      intronic chr8:141621564(-) A-I      intronic chr8:141627472(-) A-I      intronic chr8:141643127(-) A-I      intronic chr8:141546697(-) A-I      intronic chr8:141565048(-) A-I      intronic chr8:141602183(-) A-I      intronic chr8:141621609(-) A-I      intronic chr8:141609906(-) A-I      intronic chr8:141606236(-) A-I      intronic chr8:141624337(-) A-I      intronic

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	AGO2	chr8:141531836-141531837(-) m6A 3'UTR       24284625//24981863//27773535//22608085 chr8:141531892-141531893(-) m6A 3'UTR       24284625//24981863//27773535//22608085 chr8:141532057-141532058(-) m6A 3'UTR       24284625//27773535 chr8:141532071-141532072(-) m6A 3'UTR       24284625//27773535 chr8:141532234-141532235(-) m6A 3'UTR       27773535 chr8:141534949-141534950(-) m6A 3'UTR       - chr8:141535026-141535027(-) m6A 3'UTR       - chr8:141535065-141535066(-) m6A 3'UTR       - chr8:141540698-141540699(-) m6A 3'UTR       - chr8:141540720-141540721(-) m6A 3'UTR       - chr8:141540942-141540943(-) m6A 3'UTR       - chr8:141541082-141541083(-) m6A 3'UTR       - chr8:141541134-141541135(-) m6A 3'UTR       - chr8:141541386-141541387(-) m6A 3'UTR       24284625//24209618//24981863//27773535//22608085 chr8:141541427-141541428(-) m6A 3'UTR       24284625//24981863//27773535//22608085 chr8:141541472-141541473(-) m6A 3'UTR       24284625//24981863//27773535//22608085 chr8:141541541-141541542(-) m6A 3'UTR       24284625//24981863//22575960//22608085 chr8:141541692-141541693(-) m6A 3'UTR       24981863//27773535 chr8:141541718-141541719(-) m6A 3'UTR       24981863//27773535//22608085 chr8:141541756-141541757(-) m6A 3'UTR       24284625//24981863//26404942//27773535//22608085 chr8:141541830-141541831(-) m6A 3'UTR       24284625//24981863//27773535//22575960//22608085 chr8:141541845-141541846(-) m6A 3'UTR       24284625//24209618//24981863//27773535//22575960//22608085 chr8:141541857-141541858(-) m6A 3'UTR       24284625//24209618//24981863//27773535//22575960//22608085 chr8:141541890-141541891(-) m6A 3'UTR       24284625//24209618//24981863//27773535//22575960//22608085 chr8:141541943-141541944(-) m6A 3'UTR       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr8:141541955-141541956(-) m6A 3'UTR       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr8:141541962-141541963(-) m6A 3'UTR       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr8:141541975-141541976(-) m6A 3'UTR       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr8:141542041-141542042(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr8:141542081-141542082(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr8:141542096-141542097(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr8:141542170-141542171(-) m6A 3'UTR//CDS       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr8:141542209-141542210(-) m6A 3'UTR//CDS//exon       24284625//24209618//25456834//24981863//27773535//27371828//22575960//22608085 chr8:141542522-141542523(-) m6A 3'UTR//CDS//exon       24284625//24981863//27773535//22575960//22608085 chr8:141545627-141545628(-) m6A 3'UTR//CDS//exon//intron       27773535 chr8:141545651-141545652(-) m6A 3'UTR//CDS//exon//intron       27773535 chr8:141551374-141551375(-) m6A 3'UTR//CDS//exon       24284625 chr8:141551489-141551490(-) m6A exon//intron       24284625 chr8:141557639-141557640(-) m6A 3'UTR//CDS       24284625//22575960 chr8:141557651-141557652(-) m6A 3'UTR//CDS       24284625//22575960 chr8:141557704-141557705(-) m6A 3'UTR//CDS       24284625//22575960 chr8:141559271-141559272(-) m6A 3'UTR//CDS       22575960 chr8:141559304-141559305(-) m6A 3'UTR//CDS       22575960 chr8:141566330-141566331(-) m6A 3'UTR//CDS//exon       24981863//26404942 chr8:141566338-141566339(-) m6A 3'UTR//CDS       24981863//26404942 chr8:141566364-141566365(-) m6A 3'UTR//CDS       24981863//26404942//27773535 chr8:141567208-141567209(-) m6A 3'UTR//CDS       27773535 chr8:141567220-141567221(-) m6A 3'UTR//CDS       27773535 chr8:141567272-141567273(-) m6A 3'UTR//CDS       27773535//22575960 chr8:141582983-141582984(-) Gm CDS//exon       - chr8:141595289-141595290(-) m6A CDS//exon//intron       - chr8:141595323-141595324(-) m6A 5'UTR//CDS//exon//intron       - chr8:141595335-141595336(-) m6A 5'UTR//CDS//exon//intron       - chr8:141595348-141595349(-) m6A 5'UTR//CDS//exon//intron       -

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	hsa-miR-122-5p	Cytoplasm Huh-7 cells|Sorafenib-resistant Huh-7 cells (Huh-7-DR)     29253196 Cytoplasm Huh-7 cells     29486652 Cytoplasm Nasopharyngeal carcinoma cells     20498841 Endoplasmic reticulum Huh-7 cells     26304123 Exosome Breast cancer cell lines (MCF10A|MDA-MB-231)     25621950 Exosome B cell line (Raji)     21505438 Exosome Brain tissue     23382797 Exosome Plasma     23663360 Exosome Human mast cell line (HMC-1)     24009880 Exosome B cell lymphoma cell lines|EBV-transformed lymphoblastoid B cells     25242326 Exosome Blood|Chronic lymphocytic leukemia cells     - Microvesicle Plasma     23077538 Microvesicle Seminal plasma     23539611 Microvesicle Blood|Fibroblasts|Mesenchymal stem cells|Urine     - Mitochondrion Cell line (HEK-293|HeLa)     22984580 Nucleus Adjacent noncancerous tissue|HCC tissue|Huh-7 cells|Sorafenib-resistant Huh-7 cells (Huh-7-DR)     29253196 Nucleus Nasopharyngeal carcinoma cells     20498841 Polysome Human cervical cancer cell line (HeLa)     25609084
	AGO2	Chromatin K562 cells     - Cytoplasm HeLa cells     28663102 Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)|K562 cells     - Exosome Blood     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HeLa cells     28663102 Nucleus HCC cell line (HepG2)|HeLa-S3 cells     - Ribosome HEK-293 cells     22199352 Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: hsa-miR-122-5p
Interactor2: AGO2

Evidence Support:

Strong-Evidence	IP//Luciferase reporter assay//Western blot
Support Database	ViRBase

References:

PMID	23405269	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HeLa cells//Huh7 cells	Interactor2 expression	None
Description	MicroRNA-122 dependent binding of Ago2 protein to hepatitis C virus RNA is associated with enhanced RNA stability and translation stimulation. Here, we show that Argonaute(Ago) 2 protein binds to the HCV 5'-UTR in a miR-122-dependent manner, whereas the HCV 3'-UTR does not bind Ago2.