The Detail Information for This Interaction


  • Detail
  • Binding
  • Network
Detail-Interactions:
RAID ID: RRI00003888
RNA/Protein Symbol 1:MIR519A2
RNA/Protein Category 1: miRNA
RNA/Protein Symbol 2: TIMP1 mRNA
RNA/Protein Category 2: mRNA
Validated Method: Luciferase reporter assay//qRT-PCR//Western blot
Tissue: HepG2 cell line, Hep3B cell line, Huh-7 cell line, SNU398 cell line, SNU449 cell line, SNU182 cell line, SNU475 cell line 
PMID: 22262409 
Detail description:

we investigated the regulation of PTEN, CDKN1A/p21 and TIMP2 by two other C19MC mem- bers deregulated in HCCs, miR-519a and miR-519c- 3p. A direct binding of miR-519a and miR-519c-3p to the 3' UTRs of these target genes was demon- strated by a luciferase-reporter assay 

The Predicated Binding Sites between MIR519A2 and TIMP1
By miRanda Structure Match Score Energy Score MIR519A2 Location TIMP1 Location 
   Query:    3' ugugaGAUUUUCCUACGUGaaa 5'
                     || :|||  |||||   
   Ref:      5' ggaagCU-GAAGCCUGCACagu 3'
111.00 -10.28 4-18 15-35
The Predicated Binding Sites between MIR519A2 and TIMP1
By RIsearch Structure MIR519A2 Location TIMP1 Location Score 
Query:    3' GUGCAUCCUUUUAG 5'
          |||||   :||:||
Target:   5' CACGUCC-GAAGUC 3'
4-17 20-32 -7.93
The Predicated Binding Sites of TIMP1
RBPBD 

					

					

						RsiteDB
						
					

						BindN
						
							 

								
					

					

						BindN+
						
							 

								
					

					

					    RNAbindR
						
                              
	 
								
					

					

					    Pprint
						
                            

								
					



				 
	
            			
	

	
    
	
	  
	 

      

	  
Visualization:
	  
	  

	  Select the center nodes:
	   First Node
	   Second Node
	  Both the Nodes	
 
      
Select the Level of Neighbour:
	   First Neighbour
	   Second Neighbour  
	  

	        
	  

	 
	 
	


 
	 

	  The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
	  the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes. 
	  
The 'First Neighbour' represents the sub-network of direct interacting with the center node, 
	  the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node. 
	  Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
	 


	  

	   
	
	

  





	

		

           			
				
	
                   The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
 
				   The Binding page: representing the predicted binding sites and/or constants.
 
				   The Network page: representing the interaction sub-network of interacting RNA/Protein.