Detail-Interactions: | |
RAID ID: | RRI00003887 |
---|---|
RNA/Protein Symbol 1: | MIR519A1 |
RNA/Protein Category 1: | miRNA |
RNA/Protein Symbol 2: | TIMP1 mRNA |
RNA/Protein Category 2: | mRNA |
Validated Method: | Luciferase reporter assay//qRT-PCR//Western blot |
Tissue: | HepG2 cell line, Hep3B cell line, Huh-7 cell line, SNU398 cell line, SNU449 cell line, SNU182 cell line, SNU475 cell line |
PMID: | 22262409 |
Detail description: | we investigated the regulation of PTEN, CDKN1A/p21 and TIMP2 by two other C19MC mem- bers deregulated in HCCs, miR-519a and miR-519c- 3p. A direct binding of miR-519a and miR-519c-3p to the 3' UTRs of these target genes was demon- strated by a luciferase-reporter assay |
The Predicated Binding Sites between MIR519A1 and TIMP1 | |||||
By miRanda | Structure | Match Score | Energy Score | MIR519A1 Location | TIMP1 Location |
Query: 3' ugugaGAUUUUCCUACGUGaaa 5' || :||| ||||| Ref: 5' ggaagCU-GAAGCCUGCACagu 3' |
111.00 | -10.28 | 4-18 | 15-35 |
The Predicated Binding Sites between MIR519A1 and TIMP1 | ||||
By RIsearch | Structure | MIR519A1 Location | TIMP1 Location | Score |
Query: 3' GUGCAUCCUUUUAG 5' ||||| :||:|| Target: 5' CACGUCC-GAAGUC 3' |
4-17 | 20-32 | -7.93 |
The Predicated Binding Sites of TIMP1 | |
RBPBD | |
RsiteDB | |
BindN | |
BindN+ | |
RNAbindR | |
Pprint |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.