| Detail-Interactions: | |
| RAID ID: | RPI00000677 |
|---|---|
| RNA/Protein Symbol 1: | PTGS2 mRNA(COX-2) |
| RNA/Protein Category 1: | mRNA |
| RNA/Protein Symbol 2: | CELF2(CUGBP2) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Biotin Pull-down Assay, Immunocytochemistry, Luciferase Reporter Assay, Nitrocellulose Filter Binding assay, UV Cross-linking Assay |
| Tissue: | HT-29 cells |
| PMID: | 17383427 |
| Detail description: | CUGBP2 and HuR bind with similar affinities to COX-2 ARE, but CUGBP2 competes with HuR for binding. |
| The Predicated Binding Sites between PTGS2 mRNA and CELF2 | |||||
| By miRanda | Structure | Match Score | Energy Score | PTGS2 mRNA Location | CELF2 Location |
| The Predicated Binding Sites between PTGS2 mRNA and CELF2 | ||||
| By RIsearch | Structure | PTGS2 mRNA Location | CELF2 Location | Score |
| The Predicated Binding Sites of CELF2 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|O95319|CELF2_HUMAN CUGBP Elav-like family member 2 OS=Homo sapiens GN=CELF2 PE=1 SV=1 Sequence: MRCPKSAVTMRNEELLLSNGTANKMNGALDHSDQPDPDAIKMFVGQIPRSWSEKELKELF Prediction: -+--+++-+-+---------+-++-----------------------++++-++------ Confidence: 665386546457858884236356524465333343467829897274865254692899 Sequence: EPYGAVYQINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNIKTLPGMHHPIQMKP Prediction: ------------+-+++++++++---------++------------+-----------++ Confidence: 895877368364737578977674356837236563449347644852536654483765 Sequence: ADSEKSNAVEDRKLFIGMVSKKCNENDIRVMFSPFGQIEECRILRGPDGLSRGCAFVTFS Prediction: -++++++-------------++------+---------------+-----++-------- Confidence: 376575433383386556728752235869764588386772776622335763648252 Sequence: TRAMAQNAIKAMHQSQTMEGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNL Prediction: -+---+---+------+-+--++----+---+++++++++-++----------+------ Confidence: 285464487554332252634552699464257868769745627745732625522537 Sequence: TGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQLQNLATLAAAAAAAQTSATST Prediction: ------+----------+++++----------------------+--------+++++++ Confidence: 254334553499884335776633642575467774773932875776653346784887 Sequence: NANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVGLNNINALAGM Prediction: +-++-+++++-----+++---++++++------+-++--+-------+------------ Confidence: 527727666543256554443876776322342455545573545565535338377775 Sequence: AALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGP Prediction: ---------+--++-+--+-------++-----------+-+--------+-++++++-+ Confidence: 766352442623652644644652375526333875673645322554535266778424 Sequence: EGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAA Prediction: ------------------------------+-+-------+-++-----------+---- Confidence: 367397947995687889997999999477584757733244667555843733647266 Sequence: IQAMNGFQIGMKRLKVQLKRSKNDSKPY Prediction: -----------++-+-+-++++++++-+ Confidence: 7496398263458473828999898825 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|O95319|CELF2_HUMAN CUGBP Elav-like family member 2 OS=Homo sapiens GN=CELF2 PE=1 SV=1 Sequence: MRCPKSAVTMRNEELLLSNGTANKMNGALDHSDQPDPDAIKMFVGQIPRSWSEKELKELF Prediction: -+---------------------+----------------+-+-++--+----------- Confidence: 162322466323727764152423513555536655645447374576359658995999 Sequence: EPYGAVYQINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNIKTLPGMHHPIQMKP Prediction: ---------+--+++++++++++++-+-+-----------------+---------+-+- Confidence: 688785427723738577545584766857563797979657715432432141113342 Sequence: ADSEKSNAVEDRKLFIGMVSKKCNENDIRVMFSPFGQIEECRILRGPDGLSRGCAFVTFS Prediction: ------------+---++-----------------------+--+-++-+++++-+-+-- Confidence: 432212145473372435641395689959995776665286456354156958477665 Sequence: TRAMAQNAIKAMHQSQTMEGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNL Prediction: ---------------------------+---+++++++++------++------------ Confidence: 639987699667233234332314716711144347647632122446222333414427 Sequence: TGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQLQNLATLAAAAAAAQTSATST Prediction: --------------------+----------------------------------+---- Confidence: 555555454488886874633244554654556753775746976888775342144312 Sequence: NANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVGLNNINALAGM Prediction: ------------------------------------------------------------ Confidence: 353553343555546444678546354536773375464573655652255227678788 Sequence: AALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGP Prediction: ------------------------------------------------------------ Confidence: 886587788677568688768656644777564465661262534435432543332446 Sequence: EGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAA Prediction: --------+---------------------+-+----+++++++++-+-+---------- Confidence: 687272715572696688979997676554477141156765684756764565978889 Sequence: IQAMNGFQIGMKRLKVQLKRSKNDSKPY Prediction: -----------+--+-++++++-+++++ Confidence: 8567385355252373856757258847 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>O95319|CELF2 Sequence: MRCPKSAVTMRNEELLLSNGTANKMNGALDHSDQPDPDAIKMFVGQIPRSWSEKELKELF Prediction: -+--+-----------------------------+-----+-+-++--+----------- Confidence: 283474111010100100231133100100111151242163515744710000100000 Sequence: EPYGAVYQINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNIKTLPGMHHPIQMKP Prediction: ------------+-++++++++++++--+---+----------+--+--+++++++--++ Confidence: 201120010211647567757995874173035311010421262363466569853385 Sequence: ADSEKSNAVEDRKLFIGMVSKKCNENDIRVMFSPFGQIEECRILRGPDGLSRGCAFVTFS Prediction: +-+++++----------------------------------+--++++++++++++-+-- Confidence: 535667511430401023104100101010003113311436249998989889874843 Sequence: TRAMAQNAIKAMHQSQTMEGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNL Prediction: ++-------+--++-+++++++++---+---+++++--++------+------------- Confidence: 974432113532653555556776431743165866226701241153130010000100 Sequence: TGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQLQNLATLAAAAAAAQTSATST Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000000000000000000000000000000000 Sequence: NANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVGLNNINALAGM Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000000000000000000000000000000000 Sequence: AALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGP Prediction: ------------------------------------------------------------ Confidence: 000000000000001000000000000010000000000000000000000000232213 Sequence: EGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAA Prediction: +------------------------------------------+--++-+--+----+-- Confidence: 511411121001000000000000010000102010011210183355252251241544 Sequence: IQAMNGFQIGMKRLKVQLKRSKNDSKPY Prediction: -++++++++++++++-+-++++++++++- Confidence: 38568778787997748289598899880 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MRCPKSAVTMRNEELLLSNGTANKMNGALDHSDQPDPDAIKMFVGQIPRSWSEKELKELF Prediction: -++++++-----------++++++-+++-+----+---+-+++-+++++----------- Confidence: 346546632000100202353356036304331152314083909996800000000000 Sequence: EPYGAVYQINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNIKTLPGMHHPIQMKP Prediction: ------+++++++++++++++++++-+++---------------++++++++++++++++ Confidence: 000000485877979946499999909590000000000011135863489879499799 Sequence: ADSEKSNAVEDRKLFIGMVSKKCNENDIRVMFSPFGQIEECRILRGPDGLSRGCAFVTFS Prediction: +++++++++++++++++++-+------------------+++++++++++++++-+++-- Confidence: 965578999585979599928300000000000000002549979688999999094920 Sequence: TRAMAQNAIKAMHQSQTMEGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNL Prediction: -------------------------+-++++++++++++-------+---+--++++--+ Confidence: 000000000000000000110011150657899877776311322030033225553233 Sequence: TGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQLQNLATLAAAAAAAQTSATST Prediction: --+------------+----++---------------+-----------------+++++ Confidence: 013003000010002532014311010000111111231031010000110220133555 Sequence: NANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVGLNNINALAGM Prediction: +--+--------------------------------------------+----------- Confidence: 332410120212200010000100000000000001101202020323432031312102 Sequence: AALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGP Prediction: ---------++--+-+-+++++------+--+++++-++---++-+--------++++++ Confidence: 110122222552241435457331102253344444355312342420023212543578 Sequence: EGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAA Prediction: ++++-+-++--+-----------------++-+++++-+-++++++-+-+---------- Confidence: 474624044214200000000000010103427745634356997839333100000000 Sequence: IQAMNGFQIGMKRLKVQLKRSKNDSKPY Prediction: ------+++-+++++-++++++-+++++ Confidence: 0202206743896593656876236655 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.