| Detail-Interactions: | |
| RAID ID: | RPI00000658 |
|---|---|
| RNA/Protein Symbol 1: | MYC mRNA(c-myc) |
| RNA/Protein Category 1: | mRNA |
| RNA/Protein Symbol 2: | ANXA2 |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Immunoprecipitation, UV Cross-linking Assay |
| Tissue: | HeLa cells, LNCaP cells, 293 HEK cells |
| PMID: | 14672933 |
| Detail description: | Here, we show that ANXA2 binds directly to both ribonucleotide homopolymers and human c-myc RNA. |
| The Predicated Binding Sites between MYC mRNA and ANXA2 | |||||
| By miRanda | Structure | Match Score | Energy Score | MYC mRNA Location | ANXA2 Location |
| The Predicated Binding Sites between MYC mRNA and ANXA2 | ||||
| By RIsearch | Structure | MYC mRNA Location | ANXA2 Location | Score |
| The Predicated Binding Sites of ANXA2 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|P07355|ANXA2_HUMAN Annexin A2 OS=Homo sapiens GN=ANXA2 PE=1 SV=2 Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNIL Prediction: -----------------+++++-+-+-+-+-------------+--+-+----------- Confidence: 733974989394743416555827283655223464266529454652656479498364 Sequence: TNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKG Prediction: --+++-++--------++++++-----+---+-----------++--+------+---+- Confidence: 218892783665471298788543167714453286299868756165145137751472 Sequence: LGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRA Prediction: -------------+++--------+------+-----------+---+-------+-++- Confidence: 441556499686757521453592681125151485287846253446287755583982 Sequence: EDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDM Prediction: +--------------+-------+++-++-----------------------+++----- Confidence: 625389768996788569354248782866226947766136429426661167724255 Sequence: LESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDM Prediction: ----++--+-----------------------------++++++-+---+----+----- Confidence: 643766256468639797995784535815773924238796784847969472725632 Sequence: LKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD Prediction: ---+++-++++-++--------++--+-+---------- Confidence: 517766179781975112742558126267783978777 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P07355|ANXA2_HUMAN Annexin A2 OS=Homo sapiens GN=ANXA2 PE=1 SV=2 Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNIL Prediction: ----------------++-+++-----+---+-----------+---------------- Confidence: 515967877282522356133442216533243363448559443712263436899484 Sequence: TNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKG Prediction: -++++-++----------------------------------------------+-+-++ Confidence: 336563563644571221245787689256611185198778833345256355834385 Sequence: LGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRA Prediction: --------------+---------+--+---------------+---+-------+--+- Confidence: 541435789497724223432792472563233477689736261144288676733447 Sequence: EDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDM Prediction: ---------------------------------------++-------------+----- Confidence: 356464355677798289356793226488688979353561314347433312433365 Sequence: LESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDM Prediction: ----------------------------------+++-+-+-+--++--+-+-++----- Confidence: 638947893589969998885684435638973843526242511456873424824466 Sequence: LKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD Prediction: ---+---++++-++---------++--++---------- Confidence: 448435343463454425955836431347776974442 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P07355|ANXA2 Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNIL Prediction: ---------------------------+--+--+++-------++-++-+++-------- Confidence: 200001000100000000000013322624534657310111276288456732410322 Sequence: TNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKG Prediction: +++++++++--+---++---+------+---++-----------+-+-------++--++ Confidence: 577797888326334654427200411643267200300042135453400420652176 Sequence: LGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRA Prediction: --+---------++++++--+------+------------+-++++-++---------+- Confidence: 247402300323588777215104202521112100300254788736700120012472 Sequence: EDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDM Prediction: ----------------------+++++++-----++---+++++-+----+-++---+-- Confidence: 411100110000210301224456955651211057224776773742425256434740 Sequence: LESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDM Prediction: -----++-+++-++------++--+++-+--++-++--++++++-++--++++++++++- Confidence: 242245526861580013017623788494266267349867872752196578988653 Sequence: LKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD Prediction: ++-+---+++--++------+-++++--+---+--++++- Confidence: 5526113556448512411254676514701161366670 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNIL Prediction: ---------------+++++++-+-----+-+----------++----+----------- Confidence: 212010000100122676885525021104162021000000541022402121000002 Sequence: TNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKG Prediction: +++++++++--+-------------------------------+----------++++++ Confidence: 579996896104300200000000000000032000200000040200000102665798 Sequence: LGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRA Prediction: +++-----------+-++--+--------------------++++++++------++--- Confidence: 733001000000016286034000100000000010000013464948800000154221 Sequence: EDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDM Prediction: ------------------------+-------------++++---------------+-- Confidence: 000000000100000000000122510100000000106954230200001012310310 Sequence: LESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDM Prediction: --------+----------------+------+--+-++++++++++--++++++++++- Confidence: 010001203000010001000000340000023124149996877892184735987640 Sequence: LKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD Prediction: ------------+---+------+-++++------++++ Confidence: 000002000021323130000026137670000005465 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.