| Detail-Interactions: | |
| RAID ID: | RPI00000653 |
|---|---|
| RNA/Protein Symbol 1: | STAR mRNA |
| RNA/Protein Category 1: | mRNA |
| RNA/Protein Symbol 2: | AKAP1 |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation, UV Cross-linking Assay |
| Tissue: | adrenocortical carcinoma cell line H295R |
| PMID: | 23077346 |
| Detail description: | AKAP1 also interacts with STAR mRNA in a dibutyryl-cAMP-stimulated human steroidogenic adrenocortical carcinoma cell line in vivo. |
| The Predicated Binding Sites between STAR mRNA and AKAP1 | |||||
| By miRanda | Structure | Match Score | Energy Score | STAR mRNA Location | AKAP1 Location |
| The Predicated Binding Sites between STAR mRNA and AKAP1 | ||||
| By RIsearch | Structure | STAR mRNA Location | AKAP1 Location | Score |
| The Predicated Binding Sites of AKAP1 | |
| RBPBD | KHx1 |
| RsiteDB | |
| BindN |
>sp|Q92667|AKAP1_HUMAN A-kinase anchor protein 1, mitochondrial OS=Homo sapiens GN=AKAP1 PE=1 SV=1 Sequence: MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPV Prediction: ---------------------------+++++-++-----------+-+----------- Confidence: 879592398599978899999998884557682662542493653556684266277879 Sequence: EDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLR Prediction: --------+++-+-++---------++-----------+++-++++++------+--+-+ Confidence: 879773775552545535423422547546565667335452986767343322623839 Sequence: PGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQP Prediction: ++++++++++-+---+--+-++-------++-+-----++--+--+--+--++--++-+- Confidence: 867998885675577522558643736554536335425722642543623794287452 Sequence: GYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLE Prediction: --------+++++++-+-------+-------------------------++-++--+-- Confidence: 525752426889889292232222655657964998897843878959525837744553 Sequence: GEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGV Prediction: -+--++++++++----------------++--+---+---+------------+-+-+-- Confidence: 443285988876754384797498526356584273526657665374365348452645 Sequence: EGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQIISQVISEAT Prediction: --------------------------------------------++-------------- Confidence: 253552632442543344254334424332342543345243356686549935992484 Sequence: EQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLP Prediction: ---------+---+--+--+-+-++----------------+-+--+------------- Confidence: 746662264945386556263725543257374334744445453557366664565574 Sequence: LPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGI Prediction: --------+++++++-+--+---++++++++++-+-------------+----------- Confidence: 735525228755856554952545786967955455458378356762623335463977 Sequence: LVEDATCVTCMSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPEST Prediction: -------------+++------+----+-+-+++----+-++++++++++--+------+ Confidence: 698983652442355426357662422634446444235355688868673563264434 Sequence: VPFSNGVLKGELSDLGAEDGWTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGS Prediction: ----+---+--------------------++++++++--------+-+++++-+--+-++ Confidence: 744355766748238755463464736625657787543285235547654424346278 Sequence: NPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQSVQICHIEGS Prediction: ++++----------------+---++-++------++--+-+--+--+++---------- Confidence: 858784799899897258766743753955246234624864724535443737849352 Sequence: QHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNA Prediction: ------------+--+-------------+----+------------------------- Confidence: 233763983765427538483292733845667456363449998888296999965836 Sequence: GHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVV Prediction: ----------------+----------------+--+----------------------- Confidence: 737695652343447873663583762423452464439784997874653353529467 Sequence: ASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSP Prediction: -----------+---++++++-+----+--+----+------------------------ Confidence: 622452357384273754568563587647624574868478699996975747854546 Sequence: EADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYY Prediction: --------------------+++-+-------------------++---+---------- Confidence: 694674345262267563626542557568497599999999965467255783486452 Sequence: TSL Prediction: +-- Confidence: 427 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q92667|AKAP1_HUMAN A-kinase anchor protein 1, mitochondrial OS=Homo sapiens GN=AKAP1 PE=1 SV=1 Sequence: MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPV Prediction: ---------------------------+++-+-+--------------+----------- Confidence: 447491367676754568456553461543443421563797888838574145466679 Sequence: EDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLR Prediction: -----------------------------------------+++--+------------+ Confidence: 556676783756543456466734845765937486332353572333689657476144 Sequence: PGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQP Prediction: ----+--------------------------------------------------+---- Confidence: 232337564497899668571595688776773997776458776363244314252323 Sequence: GYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLE Prediction: --------------+--------------------------------------------- Confidence: 756897853431224515445744586869973899998984989999775482385355 Sequence: GEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGV Prediction: ------++-+-------------------------------------------------- Confidence: 545624631413688895997599778543494371255728789797874361336368 Sequence: EGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQIISQVISEAT Prediction: ------------------------------------------------------------ Confidence: 658893953864485686346546755655876578789677785278579978988997 Sequence: EQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLP Prediction: -------------+--+------------------------------------------- Confidence: 988997552123554353124254588679375584677643423428558998689787 Sequence: LPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGI Prediction: ------------------------------+-+--------------------------- Confidence: 887846673576332735835876563262334245367378589977673678884769 Sequence: LVEDATCVTCMSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPEST Prediction: -------------------------+---------------------------------+ Confidence: 988777986874466569757623333331543359888888385644523627442314 Sequence: VPFSNGVLKGELSDLGAEDGWTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGS Prediction: -+-++-------------------------++++-+---+-------------------- Confidence: 443452452768389878596678986733543315362384346442232234542522 Sequence: NPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQSVQICHIEGS Prediction: --------------------+---+--+--+--+-----+-------------------- Confidence: 361599799887675125724422514324346422233462762653215737727363 Sequence: QHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNA Prediction: ------------------------------------------------------------ Confidence: 145634531666158439598465955874779975354549999899298989974828 Sequence: GHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVV Prediction: --------------------------------------------------+--+------ Confidence: 735471422112225846788892955427896775389598874693884216127368 Sequence: ASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSP Prediction: ---------------+-----------+-------+------------------------ Confidence: 878887899594341424121855457466234443535378355663874665742445 Sequence: EADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYY Prediction: ------------------------------------------------------------ Confidence: 796999898485265474974145699989899999898864852556252482696577 Sequence: TSL Prediction: --- Confidence: 646 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q92667|AKAP1 Sequence: MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPV Prediction: ---+-+---------------------+---+---+------------------------ Confidence: 433705100210033124113343314844251345443100000000000000000000 Sequence: EDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLR Prediction: ------------------------------------------------------------ Confidence: 000000111122100101000000000000010000102002100101000000000001 Sequence: PGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQP Prediction: ------------------------------------------------------------ Confidence: 010100100100000100200000000000000000000000000000000222011020 Sequence: GYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLE Prediction: ------------------------------------------------------------ Confidence: 020000003000000020013311102000000000000001000000000001001000 Sequence: GEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGV Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000001000000000000000000001000100000 Sequence: EGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQIISQVISEAT Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000000000000000000000010001000110 Sequence: EQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLP Prediction: ------------------------------------------------------------ Confidence: 100101100100010010000000000000000000000000000000000000000000 Sequence: LPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGI Prediction: ------------------------------------------------------------ Confidence: 000000000000000000001000010010001000000000000000010000000000 Sequence: LVEDATCVTCMSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPEST Prediction: ------------------------------------------------------------ Confidence: 000000000100000000000000000000000000000000010000000000000100 Sequence: VPFSNGVLKGELSDLGAEDGWTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGS Prediction: ------------------------------------------+----------------- Confidence: 000010000000010100000000000000001003302113514112001000000000 Sequence: NPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQSVQICHIEGS Prediction: ---------------++--+++-++-+++-++----+----------------------- Confidence: 000000000010100563256546847783754432620101011020111221113333 Sequence: QHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNA Prediction: ------------------------------------------------------------ Confidence: 224111110200110000000000000000000000100000000000000000020020 Sequence: GHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVV Prediction: -+---+--------------------------------------+--+------++---- Confidence: 361215111112110000000000000000000001110134326246312334773423 Sequence: ASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSP Prediction: --------------+++++-----------+----+++-++----------------++- Confidence: 301010111004328855711111430401534435574854412002022132424552 Sequence: EADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYY Prediction: --------------+--+--------------------------------+--------- Confidence: 321211111113436316211312310210100201120112123000216301100010 Sequence: TSL Prediction: ---- Confidence: 0300 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPV Prediction: ---------------------------+++-++++++++-----------++-------- Confidence: 121002200000000000000000001674363547343301102011313320121100 Sequence: EDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLR Prediction: ---------------------------+--------+-++-+++++++++-+---+-+-+ Confidence: 122100000010000101010012003420010022326436686746562313042505 Sequence: PGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQP Prediction: -++++++--------------------------------+--------++--+++++-+- Confidence: 365654320102100001200112210000000000000300021322333364456153 Sequence: GYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLE Prediction: ++-+------+++++++++++-+++--------------------------------+-- Confidence: 332330001245766484565365421000000001000000001000000301121423 Sequence: GEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGV Prediction: +---+++-+++----------------------------------------------+-- Confidence: 422243425432110000000000000000010100020000000000101223132631 Sequence: EGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQIISQVISEAT Prediction: ------------------------------------------------------------ Confidence: 021000001101101220121122122112212201211010200100000000000000 Sequence: EQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLP Prediction: ------++-+-----+----++-------------------------------------- Confidence: 000000432432111431223420223220221000000010000000000000010000 Sequence: LPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGI Prediction: ------------------------++-++++-+++--+---------------------- Confidence: 000000000000001000001000352343435663340010000010000000002102 Sequence: LVEDATCVTCMSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPEST Prediction: ------------------------------------------------------------ Confidence: 100010300100001000000000000011001220022100110112230000000000 Sequence: VPFSNGVLKGELSDLGAEDGWTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGS Prediction: -----+--------------+-------++++++++--++---------------+++++ Confidence: 000003000000000000004000000264756365324322320000100032135444 Sequence: NPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQSVQICHIEGS Prediction: -+-+-----------+-------++++++++++++--+-+-+------------++-+++ Confidence: 232502000000000413122034855844763643331413120031002110332665 Sequence: QHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNA Prediction: +++-++----------------------------------------------------++ Confidence: 674247002122230010000000000000000000000000000000000000010247 Sequence: GHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVV Prediction: -+----------+-------------------------------------++-------- Confidence: 242000103311410011000000011010000000000001000013023530010201 Sequence: ASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSP Prediction: ---------------++++++-+++----------------------------------- Confidence: 002000000002003647867255410211100002021000000000000210002000 Sequence: EADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYY Prediction: ------------------------------+------------------+---------- Confidence: 000000011012101000000022330000301000000000021201240000002221 Sequence: TSL Prediction: --- Confidence: 020 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.