Basic Information
| RNALocate ID: | RLID:00006970 |
| RNA Symbol: | Ldha |
| Localization: | Cytosolic polysome |
RNA Information
| RNA Name: | LDH |
| RNA ID: | NCBI:16828|Ensembl:ENSMUSG00000063229 |
| RNA Category: | mRNA |
| Species: | Mus musculus |
| Alias: | LDH|Ldh-|Ldh1|Ldhm|l7|l7R2 |
Localization Information
| PubMed ID: | 12923260 |
| Tissue/Cell Line: | Murine plasmacytoma cells (J558) |
| Method: | ISH|S1 nuclease protection assay |
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Originally published in RNA. Lerner RS, et al, 2003. Reproduced under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) |
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| Description: | Steady-state mRNA partitioning between cytosolic and ER-bound polysomes. Total RNA was isolated from cytosol and ER membrane fractions of Jurkat and J558 cells, as indicated in Figure 1A. For each reaction, 10 μg of RNA was incubated with one of eight [32P]-labeled oligonucleotide probes representing three different classes of genes, based on the type of protein encoded: cytosolic, ER membrane, and ER lumenal. The nuclease-protected RNA fragments from parallel reactions with cytosol and ER-derived RNA were purified and separated by denaturing acrylamide gel electrophoresis. Quantification was performed by phosphorimager analysis; photostimulated luminescence output (PSL) values are indicated. Digital images of the phosphorimager output are depicted. The data are collected from Figure 4. |
Homology Information
| RNA Symbol | Gene ID | Species | Detail |
| LDHA | NCBI:3939 | Homo sapiens | Go to |
Other Subcellular Localization
| RNALocate ID | Localization | Tissue/Cell line | PubMed ID |
| RLID:00006971 | ER-bound polysome | Murine plasmacytoma cells (J558) | 12923260 |
| RLID:10000238 | Exosome | Mast cells | 17486113 |
RNA Interaction Information
| Resource | RNA Symbol | Species | Link | RNALocate ID |
| RNAInter | mmu-miR-34b-3p | Mus musculus | RR00874698 |
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