Basic Information
RNALocate ID: RLID:20000517
RNA Symbol: ACTB
Localization: Cell leading edge
RNA Information
RNA Name: β-actin
RNA ID: NCBI:396526|Ensembl:ENSGALG00000009621
RNA Category: mRNA
Species: Gallus gallus
Alias: Bact|actin
Localization Information
PubMed ID: 15923655
Tissue/Cell Line: Fibroblasts
Method: FISH
Description: Having observed that Arp2/3 complex and β-actin mRNAs are localized to the protrusions of fibroblasts, we further asked whether the mRNAs of the Arp2/3 complex physically associate with each other or with β-actin mRNA. If the subunit mRNAs of the Arp2/3 complex were co-transported, they should be found to be associated with each other.To address these questions, double detection of two types of mRNAs simultaneously in the same cells was performed by sequential FISH-TSA. As shown in Fig. 5A-C, Arp3 mRNA appears not to be precisely colocalized with β-actin mRNA, although they both concentrate together in the protrusions. In addition, the Arp2 and Arp3 mRNAs also do not colocalize, although they are both concentrated together in the protrusions (Fig. 5D-F).
Homology Information
RNA Symbol Gene ID Species Detail
ACTB NCBI:60 Homo sapiens Go to
Actb NCBI:11461 Mus musculus Go to
Actb NCBI:81822 Rattus norvegicus Go to
Other Subcellular Localization
RNALocate ID Localization Tissue/Cell line PubMed ID
RLID:00016206 Cytoplasm HEK-293T cells 27471035
RLID:00016207 Cytoplasm Human umbilical vein endothelial cells 29408197
RLID:00016208 Cytoplasm Nasopharyngeal carcinoma cells 20498841
RLID:00016209 Cytoplasm Embryonic stem cells 22193719
RLID:00016210 Cytoplasm U2OS cells 22679391
RLID:00016211 Cytosol Multidrug resistant cell line (NCI/ADR-RES) 20453113
RLID:00016212 Endoplasmic reticulum HeLa cells 15081402
RLID:00016213 Inner nuclear membrane HeLa cells 15081402
RLID:00016214 Mitochondrion Human cervical cancer cell line (HeLa) 26195734
RLID:00016215 Nucleoplasm HeLa cells 15081402
RLID:00016216 Nucleus Nasopharyngeal carcinoma cells 20498841
RLID:00016217 Outer nuclear membrane HeLa cells 15081402
RLID:00016218 Perinuclear Human T lymphocytes (Jurkat E6-1) 12923260
RLID:00016219 Ribosome Human lung fibroblasts (MRC5) 23452202
RLID:00016220 Ribosome Colon cancer cells 24393600
RLID:00016221 Ribosome HEK-293 cells 26239904
RLID:10000672 Exosome Colorectal cancer cell lines (HCT-15|SW480|WiDr) 22895844
RLID:20000518 Lamellipodium Fibroblasts 15377515
RLID:20000519 Lamellipodium Fibroblastic single cells 2095368
RLID:20000520 Protrusion Fibroblasts 15923655
RLID:20000739 Cell leading edge Human T lymphocytes (Jurkat E6-1) 12923260
RLID:20000740 Cell leading edge CRC cell line (SW480) 18535985
RLID:20000741 Lamellipodium Lung fibroblasts 23452202
RLID:20000742 RNA-rich region WI-38 cells 29133588
RLID-D:00004961 Chromatin K562 cells
RLID-D:00011811 Cytosol HeLa-S3 cells|K562 cells
RLID-D:00022315 Nucleolus K562 cells
RLID-D:00029334 Nucleoplasm K562 cells
RLID-D:00037757 Nucleus HeLa-S3 cells|K562 cells
RLID-D:10012281 Exosome Plasma|Serum
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