Basic Information
| RNALocate ID: | RLID:00017901 |
| RNA Symbol: | HSP90B1 |
| Localization: | Cytosolic polysome |
RNA Information
| RNA Name: | GRP94 |
| RNA ID: | NCBI:7184|Ensembl:ENSG00000166598 |
| RNA Category: | mRNA |
| Species: | Homo sapiens |
| Alias: | ECGP|GP96|GRP94|HEL-S-125m|HEL35|TRA1 |
Localization Information
| PubMed ID: | 12923260 |
| Tissue/Cell Line: | Human T lymphocytes (Jurkat E6-1) |
| Method: | ISH|S1 nuclease protection assay |
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Originally published in RNA. Lerner RS, et al, 2003. Reproduced under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) |
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| Description: | Steady-state mRNA partitioning between cytosolic and ER-bound polysomes. Total RNA was isolated from cytosol and ER membrane fractions of Jurkat and J558 cells, as indicated in Figure 1A. For each reaction, 10 μg of RNA was incubated with one of eight [32P]-labeled oligonucleotide probes representing three different classes of genes, based on the type of protein encoded: cytosolic, ER membrane, and ER lumenal. The nuclease-protected RNA fragments from parallel reactions with cytosol and ER-derived RNA were purified and separated by denaturing acrylamide gel electrophoresis. Quantification was performed by phosphorimager analysis; photostimulated luminescence output (PSL) values are indicated. Digital images of the phosphorimager output are depicted. The data are collected from Figure 4. |
Homology Information
| RNA Symbol | Gene ID | Species | Detail |
| Hsp90b1 | NCBI:22027 | Mus musculus | Go to |
Other Subcellular Localization
| RNALocate ID | Localization | Tissue/Cell line | PubMed ID |
| RLID:00017902 | Endoplasmic reticulum | HEK-293T cells | 21431749 |
| RLID:00017903 | Endoplasmic reticulum | Human myelogenous leukemia cell line (K-562) | 21613539 |
| RLID:00017904 | ER-bound polysome | Human T lymphocytes (Jurkat E6-1) | 12923260 |
| RLID:00017905 | Membrane-bound ribosome | Cell line (HeLa) | 18192611 |
| RLID:00017906 | Nucleus | Colon cancer cells | 24393600 |
| RLID:00017907 | Perinuclear | Human embryonic kidney cell line (HEK-293) | 12923260 |
| RLID:00017908 | Ribosome | HEK-293 cells | 22199352 |
| RLID:00017909 | Ribosome | HeLa cells | 25063809 |
| RLID-D:00005639 | Chromatin | K562 cells | |
| RLID-D:00012347 | Cytosol | K562 cells | |
| RLID-D:00022855 | Nucleolus | K562 cells | |
| RLID-D:00029995 | Nucleoplasm | K562 cells | |
| RLID-D:00038575 | Nucleus | HCC cell line (HepG2)|K562 cells | |
| RLID-D:10013809 | Exosome | Blood |
RNA Interaction Information
| Resource | RNA Symbol | Species | Link | RNALocate ID |
| RNAInter | SNHG16 | Homo sapiens | RR00726256 |
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