Basic Information
| RNALocate ID: | RLID:00008894 |
| RNA Symbol: | ALPP |
| Localization: | Endoplasmic reticulum |
RNA Information
| RNA Name: | ALPP |
| RNA ID: | NCBI:250|Ensembl:ENSG00000163283 |
| RNA Category: | mRNA |
| Species: | Homo sapiens |
| Alias: | ALP|ALPI|IAP|PALP|PLAP|PLAP-1 |
Localization Information
| PubMed ID: | 22679391 |
| Tissue/Cell Line: | COS-7 cells |
| Method: | FISH |
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The fluorescence intensities of mRNA in the ER and nucleus in the micrographs were quantified. Originally published in PLoS Biol. Cui XA, et al, 2012. Reproduced under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) |
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| Description: | ALPP and CALR, but not t-ftz or INSL3, mRNA remain associated with the ER independently of ribosomes and translation. (A-E) COS-7 cells were transfected with plasmids containing either the t-ftz (A), INSL3 (A-B), ALPP (A, C), cyto-ALPP (a version of ALPP lacking signal sequence and transmembrane domain coding regions; A, D-E), or CALR (A) genes and allowed to express mRNA for 18-24 h. The cells were then treated with DMSO (Cont), puromycin, or HHT for 30 min, and then extracted with digitonin alone or with 20 mM EDTA. Cells were then fixed, stained for mRNA using specific FISH probes, and imaged (see panels B-D for examples). The fluorescence intensities of mRNA in the ER and nucleus in the micrographs were quantified (A). Each bar represents the average and standard error of three independent experiments, each consisting of the average integrated intensity of 30 cells over background. Note that although ribosome disruption caused INSL3 mRNA to dissociate from the ER, the nuclear mRNA was unaffected (B, nuclei are denoted by arrows). (E) A single field of view containing a single HHT-treated, digitonin-extracted, COS-7 cell expressing cyto-ALPP mRNA. cyto-ALPP mRNA was visualized by FISH and for TrapĪ± protein by immunofluorescence. Note the extensive co-localization of cyto-ALPP mRNA (red) and TrapĪ± (green) in the overlay. All scale bars=20 um. Data are collected from Figure 2. |
Other Subcellular Localization
| RNALocate ID | Localization | Tissue/Cell line | PubMed ID |
| RLID:00008895 | Endoplasmic reticulum | COS-7 cells | 24019514 |
| RLID:00008896 | Endoplasmic reticulum | Glioblastoma cells (U-87) | 26272916 |
| RLID:00008897 | Nucleus | COS-7 cells | 22679391 |
| RLID:00008898 | Nucleus | COS-7 cells | 24019514 |
| RLID:00023440 | Cytoplasm | COS-7 cells | 23271194 |
| RLID:00023656 | Endoplasmic reticulum | COS-7 cells | 23271194 |
| RLID-D:10005106 | Exosome | Blood |
RNA Interaction Information
| Resource | RNA Symbol | Species | Link | RNALocate ID |
| RNAInter | AFAP1-AS1 | Homo sapiens | RR00052043 |
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