The Detail page: representing the detail information for virus-host related ncRNA interaction;
The Binding page: representing the predicted binding sites.
The Network page: representing the interaction sub-network of interacting ncRNA/Interactor.
- Detail
- Binding
- Network
| Detail: | |
| Vhid: | HHID00011999 |
|---|---|
| Virus: | Bovine viral diarrhea virus(BVDV) |
| Host Species: | Bovine |
| ncRNA Source: | Host |
| ncRNA Category: | miRNA |
| ncRNA Symbol: | bta-miR-29b |
| Tissue: | Madin-Darby bovine kidney(MDBK) cells |
| Expression Direction of ncRNA: | Upregulation |
| Interactor in Virus or Host: | Host |
| Interactor Category: | mRNA |
| Interactor Symbol: | CASP7 |
| Expression Direction of Interactor: | Downregulation |
| Validated Method: | Dual-luciferase reporter assay, Real-time PCR, Western blots analysis, Flow cytometry analysis, Rescuing of caspase-7 and NAIF1 analysis, BVDV NADL replication analysis |
| PMID: | 24965127 |
| Detailed Description: | We showed that miR-29b target sequences were present in the 3' untranslated regions of 2 key apoptosis regulators mRNAs, cysteine aspartases-7 (caspase-7) and nuclear apoptosis-inducing factor 1 (NAIF1). Indeed, upon miRNA overexpression, both mRNA and protein levels of caspase-7 and NAIF1 were decreased. We further found that miR-29b attenuated apoptosis by directly regulating intracellular levels of caspase-7 and NAIF1. Moreover, apoptosis blockage by miR-29b was rescued upon co-infection of MDBK cells with lentiviruses expressing caspase-7 and NAIF1. Importantly, miR-29b decreased BVDV NADL envelope glycoprotein E1 mRNA levels and suppressed viral replication. |
The 'First Node' or 'Second Node' option represents the sub-network of interacting ncRNA/Interactor with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting ncRNA/Interactor with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.