The Detail page: representing the detail information for virus-host related ncRNA interaction;
The Network page: representing the interaction sub-network of interacting ncRNA/Interactor.
- Detail
- Binding
- Network
| Detail: | |
| Vhid: | HVID00011972 |
|---|---|
| Virus: | Influenza A virus H1N1(H1N1) |
| Host Species: | Homo sapiens |
| ncRNA Source: | Host |
| ncRNA Category: | miRNA |
| ncRNA Symbol: | hsa-mir-323a |
| Tissue: | MDCK cells |
| Expression Direction of ncRNA: | - |
| Interactor in Virus or Host: | Virus |
| Interactor Category: | mRNA |
| Interactor Symbol: | PB1 |
| Expression Direction of Interactor: | Downregulation |
| Validated Method: | Luciferase assay, Real-time reverse transcription-PCR analysis, HA assay, Western blot assay, Fluorescence microscopy. |
| PMID: | 20554777 |
| Detailed Description: | The results showed that miRNA 323 (miR-323), miR-491, and miR-654 inhibit replication of the H1N1 influenza A virus through binding to the PB1 gene. Moreover mutational analysis of the predicted miRNA binding sites showed that the three miRNAs bind to the same conserved region of the PB1 gene. Intriguingly, despite the fact that the miRNAs and PB1 mRNA binding sequences are not a perfect match, the miRNAs downregulate PB1 expression through mRNA degradation instead of translation repression. |
The 'First Node' or 'Second Node' option represents the sub-network of interacting ncRNA/Interactor with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting ncRNA/Interactor with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.