The Detail page: representing the detail information for virus-host related ncRNA interaction;
The Binding page: representing the predicted binding sites.
The Network page: representing the interaction sub-network of interacting ncRNA/Interactor.
- Detail
- Binding
- Network
| Detail: | |
| Vhid: | HHID00011735 |
|---|---|
| Virus: | Human respiratory syncytial virus(RSV) |
| Host Species: | Homo sapiens |
| ncRNA Source: | Host |
| ncRNA Category: | miRNA |
| ncRNA Symbol: | hsa-let-7f |
| Tissue: | A549 |
| Expression Direction of ncRNA: | - |
| Interactor in Virus or Host: | Host |
| Interactor Category: | Protein |
| Interactor Symbol: | CCL7 |
| Expression Direction of Interactor: | Downregulation |
| Validated Method: | qPCR for validation of miRNA microarray data,Computational analysis of miRNA targets,Luc–UTR reporter plasmid design and Luc reporter assays,RISC immunoprecipitation assays,miRNA transfections and virus replication and plaque assays. |
| PMID: | 22894925 |
| Detailed Description: | In this study, it was shown that RSV infection of a human alveolar epithelial cell line (A549) induced five miRNAs (let-7f, miR-24, miR-337-3p, miR-26b and miR-520a-5p) and repressed two miRNAs (miR-198 and miR-595), and showed that RSV G protein triggered let-7f expression. Luciferase-untranslated region reporters and miRNA mimics and inhibitors validated the predicted targets, which included cell-cycle genes (CCND1, DYRK2 and ELF4), a chemokine gene (CCL7) and the suppressor of cytokine signalling 3 gene (SOCS3). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting ncRNA/Interactor with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting ncRNA/Interactor with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.