Basic Information:

  VIRBase ID:  

VHID00702798

  Virus:  

Human immunodeficiency virus 1 (HIV-1)

  Host:  

Homo sapiens

  Confidence Score:  

0.8589

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hiv1-miR-TAR-3p:  MIMAT0006017          IER3:  NM_003897     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hiv1-miR-TAR-3p	IER3
miRBase Accession/Entrez ID	MIMAT0006017	8870
Organism	Human immunodeficiency virus 1 (HIV-1)	Homo sapiens
Category	miRNA	mRNA
Alias	-	DIF-2//DIF2//GLY96//IEX-1//IEX-1L//IEX1//PRG1

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	IER3	chr6:30711003-30711004(-) m6A 3'UTR       24284625//27773535 chr6:30711027-30711028(-) Gm 3'UTR       - chr6:30711135-30711136(-) m6A 3'UTR       24284625//24209618//27773535 chr6:30711145-30711146(-) m6A 3'UTR       24284625//24209618//27773535 chr6:30711255-30711256(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr6:30711287-30711288(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr6:30711296-30711297(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr6:30711311-30711312(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//22575960//22608085 chr6:30711525-30711526(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr6:30711541-30711542(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711557-30711558(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711595-30711596(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711602-30711603(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711620-30711621(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711663-30711664(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711674-30711675(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711752-30711753(-) m6A 3'UTR//CDS       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085 chr6:30711764-30711765(-) m6A 3'UTR//CDS       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085 chr6:30711941-30711942(-) m6A CDS       24284625//24209618//27773535//22575960 chr6:30711946-30711947(-) m6A CDS       24284625//24209618//27773535//22575960 chr6:30712217-30712218(-) m6A CDS       24284625//25456834//24981863//27371828//22575960

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	IER3	Cytosol HCC cell line (HepG2)     - Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Ribosome-free cytosol Colon cancer cells     24393600

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID
RNAactDrug	hiv1-miR-TAR-3p	l-685,458       5479543 Pha-665752       10461815

Interaction Network (The top 100 interactions):

Interactor1: hiv1-miR-TAR-3p
Interactor2: IER3

Evidence Support:

Strong-Evidence	Luciferase reporter assay
Weak-Evidence	Microarray
Support Database	ViRBase

References:

PMID	21133815	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	293T cells	Interactor2 expression	Downregulation
Description	Microarray analysis of RNA isolated from TAR transfected cells using an Affymetrix human focus array identified 18 human genes that were downregulated and 14 genes that were upregulated. Further validation of four of these genes showed possible links to apoptosis and cell survival including ERCC1 (excision repair cross complementing-group 1), PIASg (protein inhibitor of acti-vated STATg), GIT2 (GPCR interacting protein) and IER3 (intermediate early response 3).