Basic Information:

  VIRBase ID:  

VHID00697515

  Virus:  

Human immunodeficiency virus 1 (HIV-1)

  Host:  

Homo sapiens

  Confidence Score:  

0.9797

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hiv1-miR-TAR-5p:  MIMAT0006016          IER3:  NM_003897     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hiv1-miR-TAR-5p	IER3
miRBase Accession/Entrez ID	MIMAT0006016	8870
Organism	Human immunodeficiency virus 1 (HIV-1)	Homo sapiens
Category	miRNA	mRNA
Alias	-	DIF-2//DIF2//GLY96//IEX-1//IEX-1L//IEX1//PRG1

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	IER3	chr6:30711003-30711004(-) m6A 3'UTR       24284625//27773535 chr6:30711027-30711028(-) Gm 3'UTR       - chr6:30711135-30711136(-) m6A 3'UTR       24284625//24209618//27773535 chr6:30711145-30711146(-) m6A 3'UTR       24284625//24209618//27773535 chr6:30711255-30711256(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr6:30711287-30711288(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr6:30711296-30711297(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr6:30711311-30711312(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//22575960//22608085 chr6:30711525-30711526(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr6:30711541-30711542(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711557-30711558(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711595-30711596(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711602-30711603(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711620-30711621(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711663-30711664(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711674-30711675(-) m6A 3'UTR       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr6:30711752-30711753(-) m6A 3'UTR//CDS       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085 chr6:30711764-30711765(-) m6A 3'UTR//CDS       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085 chr6:30711941-30711942(-) m6A CDS       24284625//24209618//27773535//22575960 chr6:30711946-30711947(-) m6A CDS       24284625//24209618//27773535//22575960 chr6:30712217-30712218(-) m6A CDS       24284625//25456834//24981863//27371828//22575960

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	IER3	Cytosol HCC cell line (HepG2)     - Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Ribosome-free cytosol Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: hiv1-miR-TAR-5p
Interactor2: IER3

Evidence Support:

Strong-Evidence	Luciferase reporter assay//RT-PCR//Western blot
Weak-Evidence	Microarray
Prediction-Evidence	miRanda
Support Database	ViRBase//VmiReg

References:

[1]PMID	21133815	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	293T cells	Interactor2 expression	Downregulation
Description	Microarray analysis of RNA isolated from TAR transfected cells using an Affymetrix human focus array identified 18 human genes that were downregulated and 14 genes that were upregulated. Further validation of four of these genes showed possible links to apoptosis and cell survival including ERCC1 (excision repair cross complementing-group 1), PIASg (protein inhibitor of acti-vated STATg), GIT2 (GPCR interacting protein) and IER3 (intermediate early response 3).

[2]PMID	19220914	Target region	3'UTR
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	293T cells//HLM-1 cells//U1 cells//U937 cells	Interactor2 expression	Downregulation
Description	To confirm that this repression was due to miRNA and not to any secondary effect of the procedure, RT-PCR was performed on the extracted RNA to examine the mRNA levels of ERCC1 and IER3 (Fig.10C). PCR results indicated no change in the levels of mRNA for either gene. RT-PCR on RNA extracts from 293T cells transfected with TAR-D or TAR-WT RNA also indicated no change in mRNA level. These results strongly suggested that the TAR miRNA worked by repressing the translation of targeted mRNA.