Detail Information

Basic Information:


  VIRBase ID:  

VHID00696321

  Virus:  

Human immunodeficiency virus 1 (HIV-1)

  Host:  

Homo sapiens

  Confidence Score:  

0.9806

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hiv1-miR-TAR-5p:           ERCC1:      

      *It may take a few minutes to display results.

Interactor Information:


Interactor1 Interactor2
Symbol hiv1-miR-TAR-5p ERCC1
miRBase
Accession/Entrez ID		 MIMAT0006016 2067
Organism Human immunodeficiency virus 1 (HIV-1) Homo sapiens
Category miRNA mRNA
Alias - COFS4//RAD10//UV20

RNA Editing:


Resource Symbol Editing Position Change Genetic Region
RADAR ERCC1
chr19:45914952(-) A-I      intronic
chr19:45913617(-) A-I      intronic
chr19:45913637(-) A-I      intronic
chr19:45914249(-) A-I      intronic
chr19:45913823(-) A-I      intronic
chr19:45914259(-) A-I      intronic
chr19:45914261(-) A-I      intronic
chr19:45914301(-) A-I      intronic
chr19:45914309(-) A-I      intronic
chr19:45914928(-) A-I      intronic
chr19:45913618(-) A-I      intronic
chr19:45914264(-) A-I      intronic
chr19:45913621(-) A-I      intronic
chr19:45913613(-) A-I      intronic
chr19:45915483(-) A-I      intronic
chr19:45924845(-) A-I      intronic
chr19:45915689(-) A-I      intronic
chr19:45921020(-) A-I      intronic
chr19:45921026(-) A-I      intronic
chr19:45921030(-) A-I      intronic
chr19:45924964(-) A-I      intronic
chr19:45921054(-) A-I      intronic
chr19:45921483(-) A-I      intronic
chr19:45921511(-) A-I      intronic
chr19:45921739(-) A-I      intronic
chr19:45924803(-) A-I      intronic
chr19:45913502(-) A-I      intronic
chr19:45913570(-) A-I      intronic
chr19:45915008(-) A-I      intronic
chr19:45915200(-) A-I      intronic
chr19:45914886(-) A-I      intronic
chr19:45915320(-) A-I      intronic
chr19:45925085(-) A-I      intronic
chr19:45924968(-) A-I      intronic
chr19:45914319(-) A-I      intronic
chr19:45914190(-) A-I      intronic
chr19:45915259(-) A-I      intronic
chr19:45914150(-) A-I      intronic
chr19:45914139(-) A-I      intronic
chr19:45914115(-) A-I      intronic
chr19:45913713(-) A-I      intronic
chr19:45913687(-) A-I      intronic
chr19:45914930(-) A-I      intronic
chr19:45915253(-) A-I      intronic
chr19:45925932(-) A-I      intronic
chr19:45920293(-) A-I      intronic
chr19:45921096(-) A-I      intronic
chr19:45915706(-) A-I      intronic
chr19:45924898(-) A-I      intronic
chr19:45916579(-) A-I      intronic
chr19:45915659(-) A-I      intronic
chr19:45914908(-) A-I      intronic
chr19:45925933(-) A-I      intronic
chr19:45921086(-) A-I      intronic
chr19:45914149(-) A-I      intronic
chr19:45914822(-) A-I      intronic
chr19:45925813(-) A-I      intronic
chr19:45914889(-) A-I      intronic
chr19:45914807(-) A-I      intronic
chr19:45915587(-) A-I      intronic
chr19:45925767(-) A-I      intronic
chr19:45916231(-) A-I      intronic
chr19:45915514(-) A-I      intronic
chr19:45921149(-) A-I      intronic
chr19:45921177(-) A-I      intronic
chr19:45921423(-) A-I      intronic
chr19:45921495(-) A-I      intronic
chr19:45922042(-) A-I      intronic
chr19:45916732(-) A-I      3'UTR
chr19:45921082(-) A-I      intronic
chr19:45925170(-) A-I      intronic
chr19:45914219(-) A-I      intronic
chr19:45914904(-) A-I      intronic
chr19:45921569(-) A-I      intronic
chr19:45915323(-) A-I      intronic
chr19:45921998(-) A-I      intronic
chr19:45921497(-) A-I      intronic
chr19:45914306(-) A-I      intronic
chr19:45921498(-) A-I      intronic
chr19:45919176(-) A-I      intronic
chr19:45921775(-) A-I      intronic
chr19:45915545(-) A-I      intronic
chr19:45921098(-) A-I      intronic
chr19:45925786(-) A-I      intronic
chr19:45915175(-) A-I      intronic
chr19:45920260(-) A-I      intronic
chr19:45915599(-) A-I      intronic
chr19:45924782(-) A-I      intronic
chr19:45914220(-) A-I      intronic
chr19:45916136(-) A-I      intronic
chr19:45914884(-) A-I      intronic
chr19:45915610(-) A-I      intronic
chr19:45915525(-) A-I      intronic
chr19:45914847(-) A-I      intronic
chr19:45921769(-) A-I      intronic
chr19:45921603(-) A-I      intronic
chr19:45916715(-) A-I      3'UTR
chr19:45915390(-) A-I      intronic
chr19:45914134(-) A-I      intronic
chr19:45914250(-) A-I      intronic
chr19:45921999(-) A-I      intronic
chr19:45914349(-) A-I      intronic
chr19:45915384(-) A-I      intronic
chr19:45915312(-) A-I      intronic
chr19:45915150(-) A-I      intronic
chr19:45920341(-) A-I      intronic
chr19:45914940(-) A-I      intronic
chr19:45925155(-) A-I      intronic
chr19:45914826(-) A-I      intronic
chr19:45914969(-) A-I      intronic
chr19:45921560(-) A-I      intronic
chr19:45925844(-) A-I      intronic
chr19:45925866(-) A-I      intronic
chr19:45915389(-) A-I      intronic
chr19:45914830(-) A-I      intronic
chr19:45915013(-) A-I      intronic
chr19:45915684(-) A-I      intronic
chr19:45925084(-) A-I      intronic
chr19:45925013(-) A-I      intronic
chr19:45924989(-) A-I      intronic
chr19:45914354(-) A-I      intronic
chr19:45925860(-) A-I      intronic
Resource Symbol Editing Position Change SeqReg exReg PMID
DARNED ERCC1
chr19:45926642(-) C-A intron -    21725310
chr19:45915684(-) A-G intron -    22484847

RNA Modification:


Resource Symbol ModificationPosition Type Genomic Context PMID
RMBase ERCC1
chr19:45911351-45911352(-) m6A 3'UTR       -
chr19:45911377-45911378(-) m6A 3'UTR       24284625
chr19:45911470-45911471(-) m6A 3'UTR       24284625//25456834//24981863//22575960//22608085
chr19:45911483-45911484(-) m6A 3'UTR       24284625//25456834//24981863//22575960//22608085
chr19:45911585-45911586(-) m6A 3'UTR       24284625//25456834//24981863//22608085
chr19:45911633-45911634(-) m6A 3'UTR       24284625//25456834//24981863//22608085
chr19:45911670-45911671(-) m6A 3'UTR       24284625//24981863//22608085
chr19:45911961-45911962(-) m6A 3'UTR       24284625//24209618//25456834//24981863//22575960//22608085
chr19:45912073-45912074(-) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22575960//22608085
chr19:45912078-45912079(-) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22575960//22608085
chr19:45912150-45912151(-) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22575960//22608085
chr19:45912300-45912301(-) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22575960//22608085
chr19:45912693-45912694(-) m6A 3'UTR       24284625//24209618//25456834//24981863//22575960//22608085
chr19:45912759-45912760(-) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22575960//22608085
chr19:45912911-45912912(-) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//27773535//22575960//22608085
chr19:45916702-45916703(-) m6A 3'UTR//exon//intron       24284625//25456834//24981863//27773535//27371828//22575960//22608085
chr19:45916731-45916732(-) m6A 3'UTR//exon//intron       24284625//25456834//24981863//27773535//27371828//22608085
chr19:45916749-45916750(-) m6A 3'UTR//exon//intron       24284625//25456834//24981863//27773535//27371828//22608085
chr19:45916773-45916774(-) m6A 3'UTR//exon//intron       24284625//25456834//24981863//27773535//27371828//22608085
chr19:45916826-45916827(-) m6A CDS//exon//intron       24284625//25456834//24981863//27773535//22608085
chr19:45916831-45916832(-) m6A CDS//exon//intron       24284625//25456834//24981863//27773535//22608085
chr19:45916838-45916839(-) m6A CDS//exon//intron       24284625//25456834//24981863//27773535//22608085
chr19:45916865-45916866(-) m6A CDS//exon//intron       24284625//25456834//24981863//27773535//22608085
chr19:45916877-45916878(-) m6A CDS//exon//intron       24284625//24981863//27773535//22608085
chr19:45916914-45916915(-) m6A CDS//exon//intron       27773535
chr19:45917248-45917249(-) m6A 3'UTR//CDS//exon//intron       27773536
chr19:45917615-45917616(-) m6A exon//intron       27773536
chr19:45922428-45922429(-) m6A 3'UTR//CDS//exon       27773535
chr19:45926615-45926616(-) m6A CDS//exon       27371828//27773536
chr19:45926627-45926628(-) m6A CDS//exon       27371828//27773536
chr19:45926757-45926758(-) m6A 5'UTR//intron       24981863//27371828//27773536
chr19:45926837-45926838(-) m6A 5'UTR//intron       24981863
chr19:45926845-45926846(-) m6A 5'UTR//intron       24981863
chr19:45927044-45927045(-) m6A 5'UTR//intron       24981863
chr19:45927058-45927059(-) m6A 5'UTR//intron       24981863
chr19:45927087-45927088(-) m6A 5'UTR//exon//intron       24981863
chr19:45927245-45927246(-) m6A 5'UTR//intron       24981863
chr19:45927429-45927430(-) m6A 5'UTR//intron       24981863
chr19:45927463-45927464(-) m6A 5'UTR//intron       24981863
chr19:45927523-45927524(-) m6A 5'UTR//intron       24981863
chr19:45927534-45927535(-) m6A 5'UTR//intron       24981863
chr19:45927578-45927579(-) m6A 5'UTR//intron       24981863
chr19:45927596-45927597(-) m6A 5'UTR//intron       24981863
chr19:45981874-45981875(-) m1A intron       23118484
chr19:45981877-45981878(-) Y intron       23118484
chr19:45981906-45981907(-) i6A intron       23118484
chr19:45981909-45981910(-) xU intron       23118484

RNA Localization:


Resource Symbol Subcellular Localization Tissue or Cell Line PMID
RNALocate ERCC1
Chromatin K562 cells     -
Cytosol Human myelogenous leukemia cell line (K-562)     21613539
Cytosol K562 cells     -
Exosome Blood     -
Nucleolus K562 cells     -
Nucleoplasm K562 cells     -
Nucleus HeLa-S3 cells     -
Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):


Interactor1: hiv1-miR-TAR-5p
Interactor2: ERCC1

Evidence Support:


Strong-Evidence Luciferase reporter assay//RT-PCR//Western blot
Weak-Evidence Microarray
Prediction-Evidence miRanda//Targetscan
Support Database ViRBase//VmiReg

References:


[1]PMID 21133815 Target region None
Source ViRBase Interactor1 expression None
Tissue or cell line 293T cells Interactor2 expression Downregulation
Description Cloning of these target sites into luciferase reporter constructs and analysis of reporter gene expression in the presence of TAR miRNA revealed a suppression of luciferase expression thus confirming that TAR miRNA directed downregulation of the ERCC1 gene.
[2]PMID 22936931 Target region 3'UTR
Source ViRBase Interactor1 expression None
Tissue or cell line 293T cells//HLM-1 cells//U1 cells//U937 cells Interactor2 expression Downregulation
Description Table 1 includes the RNAi pathway related gene products that are targeted by HIV-1 microRNAs. List of published HIV-1 microRNAs and their target HIV-1 and cellular gene products.The target interactions of HIV-1 microRNAs with its mRNA seem to function as viral genome regulators.
[3]PMID 19220914 Target region 3'UTR
Source ViRBase//VmiReg Interactor1 expression None
Tissue or cell line 293T cells//HLM-1 cells//U1 cells//U937 cells Interactor2 expression Downregulation
Description To confirm that this repression was due to miRNA and not to any secondary effect of the procedure, RT-PCR was performed on the extracted RNA to examine the mRNA levels of ERCC1 and IER3 (Fig.10C). PCR results indicated no change in the levels of mRNA for either gene. RT-PCR on RNA extracts from 293T cells transfected with TAR-D or TAR-WT RNA also indicated no change in mRNA level. These results strongly suggested that the TAR miRNA worked by repressing the translation of targeted mRNA.

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