A total of 36 miRNAs, including 11 known miRNAs and 25 novel miRNAs, were mapped to the PRV JS-2012 genome. We used RNAhybrid and miRanda software to scan the potential viral gene targets for these miRNAs. We scanned the 3'UTRs of PRV-encoded genes, including the LLT and found that each gene can be targeted by one or more miRNAs.