Basic Information:

  VIRBase ID:  

VHID00517070

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7903

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          PDE12:  NM_001322177     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	PDE12
miRBase Accession/Entrez ID	MIMAT0001581	201626
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	2'-PDE//2-PDE

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	PDE12	chr3:57544801(+) A-I      intronic chr3:57546192(+) A-I      3'UTR chr3:57546182(+) A-I      3'UTR chr3:57546153(+) A-I      3'UTR chr3:57546152(+) A-I      3'UTR chr3:57544357(+) A-I      intronic chr3:57544271(+) A-I      intronic chr3:57544889(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	PDE12	chr3:57544357(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	PDE12	chr3:57542193-57542194(+) m6A CDS       24284625 chr3:57542243-57542244(+) m6A CDS       24284625 chr3:57542290-57542291(+) m6A CDS       24284625 chr3:57542603-57542604(+) m6A CDS       24284625//22575960 chr3:57542651-57542652(+) m6A CDS       24284625//22575960//22608085 chr3:57542781-57542782(+) m6A CDS       24284625//25456834//24981863//22575960//22608085 chr3:57542787-57542788(+) m6A CDS       24284625//24209618//25456834//24981863//22575960//22608085 chr3:57543019-57543020(+) m6A CDS       24284625//24209618//24981863//22575960//22608085 chr3:57543033-57543034(+) m6A CDS       24284625//24209618//24981863//22575960//22608085 chr3:57543103-57543104(+) m6A CDS       24284625//24209618//24981863//22575960//22608085 chr3:57543119-57543120(+) m6A CDS       24284625//24209618//24981863//22575960//22608085 chr3:57543344-57543345(+) m6A CDS       24284625//24981863//26404942//22608085 chr3:57543356-57543357(+) m6A CDS       24284625//24981863//26404942//22608085 chr3:57545061-57545062(+) m6A CDS       24284625//24981863//22608085 chr3:57545147-57545148(+) m6A 3'UTR//intron       24284625//22608085 chr3:57545207-57545208(+) m6A 3'UTR//intron       24284625//22608085 chr3:57545244-57545245(+) m6A 3'UTR//intron       24284625//24981863//22608085 chr3:57545337-57545338(+) m6A 3'UTR//CDS       24284625//24209618//24981863//22608085 chr3:57545387-57545388(+) m6A 3'UTR//CDS       24284625//24981863//26404942//22608085 chr3:57545459-57545460(+) m6A 3'UTR//CDS       24284625//24209618//24981863//26404942//22608085 chr3:57545538-57545539(+) m6A 3'UTR//CDS       24284625//24209618//24981863//22608085 chr3:57545619-57545620(+) m6A 3'UTR//CDS       24284625//24981863//22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	PDE12	Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytosol K562 cells     - Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus Colon cancer cells     24393600 Nucleus HeLa-S3 cells|K562 cells     -

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: PDE12

Evidence Support:

Strong-Evidence	RISC-IP
Prediction-Evidence	miRanda//PITA
Support Database	ViRBase//VmiReg

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.