Basic Information:

  VIRBase ID:  

VHID00516483

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          NFAM1:  NM_145912     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	NFAM1
miRBase Accession/Entrez ID	MIMAT0001581	150372
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	CNAIP

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	NFAM1	chr22:42777195(-) A-I      3'UTR chr22:42779465(-) A-I      3'UTR chr22:42779466(-) A-I      3'UTR chr22:42779576(-) A-I      3'UTR chr22:42779509(-) A-I      3'UTR chr22:42779502(-) A-I      3'UTR chr22:42807151(-) A-I      intronic chr22:42779626(-) A-I      3'UTR chr22:42779635(-) A-I      3'UTR chr22:42819056(-) A-I      intronic chr22:42819062(-) A-I      intronic chr22:42819037(-) A-I      intronic chr22:42819036(-) A-I      intronic chr22:42819010(-) A-I      intronic chr22:42819007(-) A-I      intronic chr22:42807126(-) A-I      intronic chr22:42807139(-) A-I      intronic chr22:42816137(-) A-I      intronic chr22:42820309(-) A-I      intronic chr22:42820914(-) A-I      intronic chr22:42821065(-) A-I      intronic chr22:42821072(-) A-I      intronic chr22:42824559(-) A-I      intronic chr22:42826209(-) A-I      intronic chr22:42826206(-) A-I      intronic chr22:42825510(-) A-I      intronic chr22:42824576(-) A-I      intronic chr22:42821088(-) A-I      intronic chr22:42798939(-) A-I      intronic chr22:42798940(-) A-I      intronic chr22:42799049(-) A-I      intronic chr22:42799110(-) A-I      intronic chr22:42802878(-) A-I      intronic chr22:42802879(-) A-I      intronic chr22:42802886(-) A-I      intronic chr22:42802919(-) A-I      intronic chr22:42803010(-) A-I      intronic chr22:42807154(-) A-I      intronic chr22:42816240(-) A-I      intronic chr22:42796618(-) A-I      intronic chr22:42820402(-) A-I      intronic chr22:42824572(-) A-I      intronic chr22:42807127(-) A-I      intronic chr22:42794516(-) A-I      intronic chr22:42826267(-) A-I      intronic chr22:42803153(-) A-I      intronic chr22:42826222(-) A-I      intronic chr22:42811414(-) A-I      intronic chr22:42794515(-) A-I      intronic chr22:42789300(-) A-I      intronic chr22:42800739(-) A-I      intronic chr22:42791449(-) A-I      intronic chr22:42794557(-) A-I      intronic chr22:42821217(-) A-I      intronic chr22:42799064(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	NFAM1	chr22:42776588(-) A-I exon 3'UTR    15342557 chr22:42777105(-) A-I exon 3'UTR    15342557   //15258596 chr22:42777153(-) A-I exon 3'UTR    15342557 chr22:42777158(-) A-I exon 3'UTR    15342557   //15258596 chr22:42777161(-) A-I exon 3'UTR    15342557 chr22:42777186(-) A-I exon 3'UTR    15342557   //15258596 chr22:42777190(-) A-I exon 3'UTR    15342557 chr22:42777194(-) A-I exon 3'UTR    15342557   //15258596 chr22:42777244(-) A-I exon 3'UTR    15342557   //15258596 chr22:42777257(-) A-I exon 3'UTR    15342557   //15258596 chr22:42777269(-) A-I exon 3'UTR    15342557 chr22:42777866(-) A-I exon 3'UTR    15342557 chr22:42778210(-) A-I exon 3'UTR    15342557 chr22:42778662(-) A-I exon 3'UTR    15342557 chr22:42778774(-) A-I exon 3'UTR    15342557 chr22:42778776(-) A-I exon 3'UTR    15342557 chr22:42778789(-) A-I exon 3'UTR    15342557 chr22:42778837(-) A-I exon 3'UTR    15342557 chr22:42779018(-) A-I exon 3'UTR    15342557 chr22:42779432(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779476(-) A-I exon 3'UTR    15342557 chr22:42779481(-) A-I exon 3'UTR    15342557 chr22:42779487(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779490(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779501(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779507(-) A-I exon 3'UTR    15342557 chr22:42779511(-) A-I exon 3'UTR    15342557 chr22:42779514(-) A-I exon 3'UTR    15342557 chr22:42779515(-) A-I exon 3'UTR    15342557 chr22:42779539(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779540(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779551(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779555(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779560(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779562(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779578(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779606(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779609(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779624(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779637(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779653(-) A-I exon 3'UTR    15342557   //15258596 chr22:42779694(-) A-I exon 3'UTR    15258596 chr22:42779699(-) A-I exon 3'UTR    15342557 chr22:42777105(-) A-G exon 3'UTR    22484847 chr22:42779637(-) A-G exon 3'UTR    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	NFAM1	chr22:42778578-42778579(-) m6A 3'UTR       27371828 chr22:42778589-42778590(-) m6A 3'UTR       27371828 chr22:42778632-42778633(-) m6A 3'UTR       27371828 chr22:42778664-42778665(-) m6A 3'UTR       27371828 chr22:42778681-42778682(-) m6A 3'UTR       25456834//27371828 chr22:42778709-42778710(-) m6A 3'UTR       25456834//27371828 chr22:42778731-42778732(-) m6A 3'UTR       25456834//27371828 chr22:42778780-42778781(-) m6A 3'UTR       27371828 chr22:42779270-42779271(-) m6A 3'UTR       - chr22:42779285-42779286(-) m6A 3'UTR       - chr22:42779939-42779940(-) m6A 3'UTR       - chr22:42780402-42780403(-) m6A 3'UTR       27371828 chr22:42780408-42780409(-) m6A 3'UTR       27371828 chr22:42780437-42780438(-) m6A 3'UTR       25456834//27371828 chr22:42780542-42780543(-) m6A 3'UTR       25456834//27371828 chr22:42780561-42780562(-) m6A 3'UTR       27371828 chr22:42780574-42780575(-) m6A 3'UTR       27371828 chr22:42780590-42780591(-) m6A 3'UTR       27371828 chr22:42780607-42780608(-) m6A 3'UTR       27371828 chr22:42780759-42780760(-) m6A 3'UTR       27371828 chr22:42781065-42781066(-) m6A 3'UTR       - chr22:42781098-42781099(-) m6A 3'UTR       - chr22:42781124-42781125(-) m6A 3'UTR       - chr22:42781193-42781194(-) m6A CDS       - chr22:42807525-42807526(-) m6A CDS       - chr22:42807543-42807544(-) m6A CDS       - chr22:42807557-42807558(-) m6A CDS       - chr22:42828355-42828356(-) m6A CDS       -

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	NFAM1	Exosome Blood     -

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: NFAM1

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.