Basic Information:

  VIRBase ID:  

VHID00516415

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          BROX:  NR_109927     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	BROX
miRBase Accession/Entrez ID	MIMAT0001581	148362
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	C1orf58

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	BROX	chr1:222888156(+) A-I      intronic chr1:222888801(+) A-I      intronic chr1:222888840(+) A-I      intronic chr1:222899197(+) A-I      intronic chr1:222888841(+) A-I      intronic chr1:222888144(+) A-I      intronic chr1:222893934(+) A-I      intronic chr1:222893953(+) A-I      intronic chr1:222899089(+) A-I      intronic chr1:222899173(+) A-I      intronic chr1:222888169(+) A-I      intronic chr1:222895784(+) A-I      NonSyn:Glu->Gly chr1:222894035(+) A-I      intronic chr1:222888132(+) A-I      intronic chr1:222897162(+) A-I      intronic chr1:222899148(+) A-I      intronic chr1:222888136(+) A-I      intronic chr1:222894032(+) A-I      intronic chr1:222888157(+) A-I      intronic chr1:222897269(+) A-I      intronic chr1:222897239(+) A-I      intronic chr1:222897222(+) A-I      intronic chr1:222900029(+) A-I      intronic chr1:222897150(+) A-I      intronic chr1:222897160(+) A-I      intronic chr1:222897223(+) A-I      intronic chr1:222888830(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	BROX	chr1:222888136(+) A-I intron -    21960545   //22327324 chr1:222888157(+) A-I intron -    21960545 chr1:222888132(+) A-G intron -    22484847 chr1:222888136(+) A-G intron -    22484847 chr1:222888157(+) A-G intron -    22484847 chr1:222888830(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	BROX	chr1:222885993-222885994(+) m6A 5'UTR//exon       24284625//24981863 chr1:222886060-222886061(+) m6A 5'UTR//exon       24284625//24981863 chr1:222886257-222886258(+) m6A 5'UTR//exon       24981863 chr1:222886265-222886266(+) m6A 5'UTR//exon       24981863 chr1:222886719-222886720(+) m6A 5'UTR//exon//intron       24981863 chr1:222886931-222886932(+) m6A exon//intron       24981863 chr1:222886941-222886942(+) m6A exon//intron       24981863 chr1:222886948-222886949(+) m6A exon//intron       24981863 chr1:222886991-222886992(+) m6A exon//intron       24981863 chr1:222897496-222897497(+) m6A CDS//exon       24981863 chr1:222898808-222898809(+) m6A CDS//exon       24981863 chr1:222900531-222900532(+) m6A CDS//exon       24981863 chr1:222904804-222904805(+) m6A CDS//intron       22608085 chr1:222904813-222904814(+) m6A CDS//intron       22608085 chr1:222904842-222904843(+) m6A CDS//intron       24981863//27773535//22608085 chr1:222905974-222905975(+) m6A CDS       24981863//27773535//22608085 chr1:222905980-222905981(+) m6A CDS       24981863//27773535//22608085 chr1:222905998-222905999(+) m6A 3'UTR//CDS       24981863//22608085 chr1:222906010-222906011(+) m6A 3'UTR//CDS       24981863//22608085 chr1:222906015-222906016(+) m6A 3'UTR//CDS       24981863//22608085 chr1:222906022-222906023(+) m6A 3'UTR//CDS       24981863//22608085 chr1:222906033-222906034(+) m6A 3'UTR//CDS       24981863//22608085 chr1:222906100-222906101(+) m6A 3'UTR       24981863

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	BROX	Cytosol HCC cell line (HepG2)     - Exosome Blood     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     -

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: BROX

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.