Basic Information:

  VIRBase ID:  

VHID00512671

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          USP28:  NM_001346263     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	USP28
miRBase Accession/Entrez ID	MIMAT0001581	57646
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	-

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	USP28	chr11:113708417(-) A-I      intronic chr11:113708416(-) A-I      intronic chr11:113669085(-) A-I      3'UTR chr11:113669074(-) A-I      3'UTR chr11:113669073(-) A-I      3'UTR chr11:113669027(-) A-I      3'UTR chr11:113669025(-) A-I      3'UTR chr11:113669015(-) A-I      3'UTR chr11:113689483(-) A-I      intronic chr11:113708415(-) A-I      intronic chr11:113708461(-) A-I      intronic chr11:113689333(-) A-I      intronic chr11:113689321(-) A-I      intronic chr11:113689106(-) A-I      intronic chr11:113689050(-) A-I      intronic chr11:113689048(-) A-I      intronic chr11:113690509(-) A-I      intronic chr11:113722895(-) A-I      intronic chr11:113689043(-) A-I      intronic chr11:113689042(-) A-I      intronic chr11:113689908(-) A-I      intronic chr11:113689012(-) A-I      intronic chr11:113688971(-) A-I      intronic chr11:113688970(-) A-I      intronic chr11:113688966(-) A-I      intronic chr11:113688926(-) A-I      intronic chr11:113688912(-) A-I      intronic chr11:113688911(-) A-I      intronic chr11:113688909(-) A-I      intronic chr11:113697196(-) A-I      intronic chr11:113687153(-) A-I      intronic chr11:113687093(-) A-I      intronic chr11:113689412(-) A-I      intronic chr11:113682244(-) A-I      intronic chr11:113695627(-) A-I      intronic chr11:113681568(-) A-I      intronic chr11:113681565(-) A-I      intronic chr11:113723017(-) A-I      intronic chr11:113681508(-) A-I      intronic chr11:113681482(-) A-I      intronic chr11:113676878(-) A-I      intronic chr11:113700431(-) A-I      intronic chr11:113689399(-) A-I      intronic chr11:113722822(-) A-I      intronic chr11:113740029(-) A-I      intronic chr11:113681570(-) A-I      intronic chr11:113681593(-) A-I      intronic chr11:113722883(-) A-I      intronic chr11:113723023(-) A-I      intronic chr11:113690476(-) A-I      intronic chr11:113689918(-) A-I      intronic chr11:113726594(-) A-I      intronic chr11:113716375(-) A-I      intronic chr11:113715733(-) A-I      intronic chr11:113702970(-) A-I      intronic chr11:113722811(-) A-I      intronic chr11:113725261(-) A-I      intronic chr11:113700484(-) A-I      intronic chr11:113695620(-) A-I      intronic chr11:113697519(-) A-I      intronic chr11:113722889(-) A-I      intronic chr11:113689870(-) A-I      intronic chr11:113689336(-) A-I      intronic chr11:113689973(-) A-I      intronic chr11:113695614(-) A-I      intronic chr11:113695553(-) A-I      intronic chr11:113697472(-) A-I      intronic chr11:113700464(-) A-I      intronic chr11:113695410(-) A-I      intronic chr11:113695409(-) A-I      intronic chr11:113689539(-) A-I      intronic chr11:113726421(-) A-I      intronic chr11:113732947(-) A-I      intronic chr11:113734284(-) A-I      intronic chr11:113695408(-) A-I      intronic chr11:113690030(-) A-I      intronic chr11:113692999(-) A-I      intronic chr11:113706544(-) A-I      intronic chr11:113715780(-) A-I      intronic chr11:113692992(-) A-I      intronic chr11:113681579(-) A-I      intronic chr11:113716474(-) A-I      intronic chr11:113692980(-) A-I      intronic chr11:113690606(-) A-I      intronic chr11:113690570(-) A-I      intronic chr11:113689581(-) A-I      intronic chr11:113716479(-) A-I      intronic chr11:113716498(-) A-I      intronic chr11:113689547(-) A-I      intronic chr11:113716561(-) A-I      intronic chr11:113689507(-) A-I      intronic chr11:113722780(-) A-I      intronic chr11:113689501(-) A-I      intronic chr11:113722781(-) A-I      intronic chr11:113689500(-) A-I      intronic chr11:113689471(-) A-I      intronic chr11:113697206(-) A-I      intronic chr11:113668973(-) A-I      3'UTR chr11:113693281(-) A-I      intronic chr11:113689932(-) A-I      intronic chr11:113716443(-) A-I      intronic chr11:113725343(-) A-I      intronic chr11:113681449(-) A-I      intronic chr11:113716583(-) A-I      intronic chr11:113709962(-) A-I      intronic chr11:113697256(-) A-I      intronic chr11:113709959(-) A-I      intronic chr11:113703550(-) A-I      intronic chr11:113680498(-) A-I      intronic chr11:113697170(-) A-I      intronic chr11:113725351(-) A-I      intronic chr11:113689887(-) A-I      intronic chr11:113708422(-) A-I      intronic chr11:113690510(-) A-I      intronic chr11:113702999(-) A-I      intronic chr11:113671000(-) A-I      intronic chr11:113693088(-) A-I      intronic chr11:113681574(-) A-I      intronic chr11:113693286(-) A-I      intronic chr11:113739992(-) A-I      intronic chr11:113689920(-) A-I      intronic chr11:113690023(-) A-I      intronic chr11:113716017(-) A-I      intronic chr11:113689038(-) A-I      intronic chr11:113695515(-) A-I      intronic chr11:113695273(-) A-I      intronic chr11:113695278(-) A-I      intronic chr11:113729872(-) A-I      intronic chr11:113689907(-) A-I      intronic chr11:113715903(-) A-I      intronic chr11:113715785(-) A-I      intronic chr11:113690107(-) A-I      intronic chr11:113690538(-) A-I      intronic chr11:113682240(-) A-I      intronic chr11:113678068(-) A-I      intronic chr11:113693235(-) A-I      intronic chr11:113690034(-) A-I      intronic chr11:113690494(-) A-I      intronic chr11:113689435(-) A-I      intronic chr11:113726428(-) A-I      intronic chr11:113743087(-) A-I      intronic chr11:113689869(-) A-I      intronic chr11:113697271(-) A-I      intronic chr11:113689927(-) A-I      intronic chr11:113689461(-) A-I      intronic chr11:113708392(-) A-I      intronic chr11:113726511(-) A-I      intronic chr11:113671693(-) A-I      intronic chr11:113700540(-) A-I      intronic chr11:113700392(-) A-I      intronic chr11:113689526(-) A-I      intronic chr11:113710050(-) A-I      intronic chr11:113735707(-) A-I      intronic chr11:113690108(-) A-I      intronic chr11:113689946(-) A-I      intronic chr11:113708391(-) A-I      intronic chr11:113690114(-) A-I      intronic chr11:113693309(-) A-I      intronic chr11:113689475(-) A-I      intronic chr11:113690544(-) A-I      intronic chr11:113708386(-) A-I      intronic chr11:113726550(-) A-I      intronic chr11:113695544(-) A-I      intronic chr11:113700490(-) A-I      intronic chr11:113682230(-) A-I      intronic chr11:113690580(-) A-I      intronic chr11:113725229(-) A-I      intronic chr11:113734880(-) A-I      intronic chr11:113703048(-) A-I      intronic chr11:113693308(-) A-I      intronic chr11:113723022(-) A-I      intronic chr11:113695591(-) A-I      intronic chr11:113689410(-) A-I      intronic chr11:113708414(-) A-I      intronic chr11:113682408(-) A-I      intronic chr11:113681576(-) A-I      intronic chr11:113681575(-) A-I      intronic chr11:113690014(-) A-I      intronic chr11:113728781(-) A-I      intronic chr11:113697517(-) A-I      intronic chr11:113706546(-) A-I      intronic chr11:113695177(-) A-I      intronic chr11:113735723(-) A-I      intronic chr11:113700383(-) A-I      intronic chr11:113689999(-) A-I      intronic chr11:113700495(-) A-I      intronic chr11:113697186(-) A-I      intronic chr11:113681525(-) A-I      intronic chr11:113700452(-) A-I      intronic chr11:113689434(-) A-I      intronic chr11:113690574(-) A-I      intronic chr11:113722418(-) A-I      intronic chr11:113708432(-) A-I      intronic chr11:113697202(-) A-I      intronic chr11:113720760(-) A-I      intronic chr11:113682247(-) A-I      intronic chr11:113689931(-) A-I      intronic chr11:113689867(-) A-I      intronic chr11:113695178(-) A-I      intronic chr11:113669090(-) A-I      3'UTR

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	USP28	chr11:113689867(-) A-I intron -    15342557 chr11:113689869(-) A-I intron -    15342557 chr11:113689870(-) A-I intron -    15342557 chr11:113689887(-) A-I intron -    15342557 chr11:113689907(-) A-I intron -    15342557 chr11:113689908(-) A-I intron -    15342557 chr11:113689918(-) A-I intron -    15342557 chr11:113689920(-) A-I intron -    15342557 chr11:113689927(-) A-I intron -    15342557 chr11:113689931(-) A-I intron -    15342557 chr11:113689932(-) A-I intron -    15342557 chr11:113689946(-) A-I intron -    15342557 chr11:113689973(-) A-I intron -    15342557 chr11:113690023(-) A-I intron -    15342557 chr11:113690034(-) A-I intron -    15342557 chr11:113690107(-) A-I intron -    15342557 chr11:113690108(-) A-I intron -    15342557 chr11:113690114(-) A-I intron -    15342557 chr11:113690494(-) A-I intron -    15342557 chr11:113690509(-) A-I intron -    15342557 chr11:113690510(-) A-I intron -    15342557 chr11:113690538(-) A-I intron -    15342557 chr11:113690574(-) A-I intron -    15342557 chr11:113690580(-) A-I intron -    15342557 chr11:113668814(-) C-A exon 3'UTR    21725310 chr11:113681574(-) A-G intron -    22484847 chr11:113681575(-) A-G intron -    22484847 chr11:113681576(-) A-G intron -    22484847 chr11:113681579(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	USP28	chr11:113669842-113669843(-) m6A 3'UTR//exon       24284625//24981863//27773535//22608085 chr11:113670099-113670100(-) m6A 3'UTR//CDS//exon       27773535 chr11:113672239-113672240(-) m6A 3'UTR//CDS//exon       27773535 chr11:113675439-113675440(-) m6A 3'UTR//CDS       24981863 chr11:113675624-113675625(-) m6A 3'UTR//CDS       24981863 chr11:113675635-113675636(-) m6A 3'UTR//CDS       24981863 chr11:113675722-113675723(-) m6A 3'UTR//CDS       24981863 chr11:113691604-113691605(-) m6A intron       22608085 chr11:113691619-113691620(-) m6A intron       22608085 chr11:113691656-113691657(-) m6A intron       22608085 chr11:113691954-113691955(-) m6A intron       22608085 chr11:113691962-113691963(-) m6A intron       22608085 chr11:113692063-113692064(-) m6A intron       25456834//22608085 chr11:113692070-113692071(-) m6A intron       25456834//22608085 chr11:113692159-113692160(-) m6A intron       22608085 chr11:113692189-113692190(-) m6A intron       22608085 chr11:113697957-113697958(-) m6A 3'UTR//CDS       27773535 chr11:113725034-113725035(-) Y CDS//exon//intron       26075521 chr11:113745469-113745470(-) m6A 5'UTR//intron       22608085 chr11:113745503-113745504(-) m6A 5'UTR//intron       22608085 chr11:113746252-113746253(-) m6A 5'UTR//exon       24981863//27773536

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	USP28	Chromatin K562 cells     - Cytosol HCC cell line (HepG2)     - Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)     -

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: USP28

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.