Basic Information:

  VIRBase ID:  

VHID00510204

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          PDCD4:  NM_145341     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	PDCD4
miRBase Accession/Entrez ID	MIMAT0001581	27250
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	H731

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	PDCD4	chr10:112650922(+) A-I      intronic chr10:112636041(+) A-I      intronic chr10:112639276(+) A-I      intronic chr10:112650998(+) A-I      intronic chr10:112639286(+) A-I      intronic chr10:112635876(+) A-I      intronic chr10:112635992(+) A-I      intronic chr10:112635991(+) A-I      intronic chr10:112635900(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	PDCD4	chr10:112639276(+) A-G intron -    22484847 chr10:112639286(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	PDCD4	chr10:112631658-112631659(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr10:112631664-112631665(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr10:112631689-112631690(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr10:112631711-112631712(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr10:112631745-112631746(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr10:112635819-112635820(+) m6A 5'UTR//CDS//exon//intron       24209618//24981863 chr10:112641043-112641044(+) m6A CDS//exon       22608085 chr10:112641143-112641144(+) m6A CDS//exon       24981863//22575960//22608085 chr10:112641155-112641156(+) m6A CDS//exon       24981863//22575960//22608085 chr10:112641279-112641280(+) m6A CDS//exon       24981863//22575960//22608085 chr10:112642797-112642798(+) m6A CDS//exon       24981863//27773535//22575960 chr10:112645028-112645029(+) m6A CDS//exon       22575960 chr10:112645067-112645068(+) m6A CDS//exon       22575960 chr10:112657830-112657831(+) m6A CDS//exon       26404942 chr10:112657854-112657855(+) m6A 3'UTR//exon       26404942 chr10:112658366-112658367(+) m6A 3'UTR//exon       27773535 chr10:112658388-112658389(+) m6A 3'UTR//exon       27773535 chr10:112658393-112658394(+) m6A 3'UTR//exon       27773535 chr10:112658417-112658418(+) m6A 3'UTR//exon       27773535 chr10:112658436-112658437(+) m6A 3'UTR//exon       27773535 chr10:112658449-112658450(+) m6A 3'UTR//exon       27773535 chr10:112658482-112658483(+) m6A 3'UTR       27773535 chr10:112658537-112658538(+) m6A 3'UTR       27773535 chr10:112658587-112658588(+) m6A 3'UTR       27773535 chr10:112658621-112658622(+) m6A 3'UTR       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	PDCD4	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)     - Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleus HCC cell line (HepG2)     - Ribosome HEK-293 cells     22199352

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: PDCD4

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.