Basic Information:

  VIRBase ID:  

VHID00510038

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          CKAP2:  NM_018204     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	CKAP2
miRBase Accession/Entrez ID	MIMAT0001581	26586
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	LB1//se20-10//TMAP

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	CKAP2	chr13:53032913(+) A-I      intronic chr13:53032811(+) A-I      intronic chr13:53032845(+) A-I      intronic chr13:53034232(+) A-I      intronic chr13:53031128(+) A-I      intronic chr13:53031052(+) A-I      intronic chr13:53040850(+) A-I      intronic chr13:53033597(+) A-I      intronic chr13:53032812(+) A-I      intronic chr13:53032822(+) A-I      intronic chr13:53033600(+) A-I      intronic chr13:53031127(+) A-I      intronic chr13:53031829(+) A-I      intronic chr13:53041678(+) A-I      intronic chr13:53031787(+) A-I      intronic chr13:53034029(+) A-I      intronic chr13:53031830(+) A-I      intronic chr13:53045234(+) A-I      intronic chr13:53045222(+) A-I      intronic chr13:53045214(+) A-I      intronic chr13:53042144(+) A-I      intronic chr13:53041783(+) A-I      intronic chr13:53031177(+) A-I      intronic chr13:53042152(+) A-I      intronic chr13:53041995(+) A-I      intronic chr13:53031835(+) A-I      intronic chr13:53041765(+) A-I      intronic chr13:53034730(+) A-I      intronic chr13:53041733(+) A-I      intronic chr13:53037258(+) A-I      intronic chr13:53034769(+) A-I      intronic chr13:53031839(+) A-I      intronic chr13:53034203(+) A-I      intronic chr13:53041684(+) A-I      intronic chr13:53034107(+) A-I      intronic chr13:53034099(+) A-I      intronic chr13:53032743(+) A-I      intronic chr13:53031846(+) A-I      intronic chr13:53031828(+) A-I      intronic chr13:53032839(+) A-I      intronic chr13:53042086(+) A-I      intronic chr13:53031869(+) A-I      intronic chr13:53032867(+) A-I      intronic chr13:53042075(+) A-I      intronic chr13:53041134(+) A-I      intronic chr13:53031060(+) A-I      intronic chr13:53031086(+) A-I      intronic chr13:53031832(+) A-I      intronic chr13:53032851(+) A-I      intronic chr13:53031215(+) A-I      intronic chr13:53031765(+) A-I      intronic chr13:53031770(+) A-I      intronic chr13:53034654(+) A-I      intronic chr13:53044423(+) A-I      intronic chr13:53031812(+) A-I      intronic chr13:53038510(+) A-I      intronic chr13:53044393(+) A-I      intronic chr13:53031197(+) A-I      intronic chr13:53041622(+) A-I      intronic chr13:53037102(+) A-I      intronic chr13:53041847(+) A-I      intronic chr13:53041656(+) A-I      intronic chr13:53033513(+) A-I      intronic chr13:53041751(+) A-I      intronic chr13:53034089(+) A-I      intronic chr13:53040957(+) A-I      intronic chr13:53040982(+) A-I      intronic chr13:53041682(+) A-I      intronic chr13:53040976(+) A-I      intronic chr13:53042127(+) A-I      intronic chr13:53041140(+) A-I      intronic chr13:53041022(+) A-I      intronic chr13:53041681(+) A-I      intronic chr13:53037142(+) A-I      intronic chr13:53047133(+) A-I      intronic chr13:53038509(+) A-I      intronic chr13:53040852(+) A-I      intronic chr13:53032847(+) A-I      intronic chr13:53041590(+) A-I      intronic chr13:53041621(+) A-I      intronic chr13:53037270(+) A-I      intronic chr13:53041724(+) A-I      intronic chr13:53042130(+) A-I      intronic chr13:53041993(+) A-I      intronic chr13:53045128(+) A-I      intronic chr13:53032894(+) A-I      intronic chr13:53040993(+) A-I      intronic chr13:53032898(+) A-I      intronic chr13:53033587(+) A-I      intronic chr13:53040953(+) A-I      intronic chr13:53044264(+) A-I      intronic chr13:53043993(+) A-I      intronic chr13:53041047(+) A-I      intronic chr13:53034055(+) A-I      intronic chr13:53033726(+) A-I      intronic chr13:53041723(+) A-I      intronic chr13:53037335(+) A-I      intronic chr13:53041786(+) A-I      intronic chr13:53034112(+) A-I      intronic chr13:53034167(+) A-I      intronic chr13:53038432(+) A-I      intronic chr13:53034139(+) A-I      intronic chr13:53034635(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	CKAP2	chr13:53037142(+) A-I intron -    22327324 chr13:53037142(+) A-G intron -    22484847 chr13:53037270(+) A-G intron -    22484847 chr13:53040982(+) A-G intron -    22484847 chr13:53041022(+) A-G intron -    22484847 chr13:53041723(+) A-G intron -    22484847 chr13:53041751(+) A-G intron -    22484847 chr13:53041783(+) A-G intron -    22484847 chr13:53045128(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	CKAP2	chr13:53029616-53029617(+) m6A 5'UTR       24284625//24981863//22608085 chr13:53029716-53029717(+) m6A 5'UTR//CDS       24284625//24981863 chr13:53030667-53030668(+) m6A 5'UTR//CDS       24981863 chr13:53030673-53030674(+) m6A 5'UTR//CDS       24284625//24981863 chr13:53030682-53030683(+) m6A 5'UTR//CDS       24284625//24981863 chr13:53035062-53035063(+) m6A CDS//intron       24284625//24981863//27773535//22608085 chr13:53035079-53035080(+) m6A CDS//intron       24284625//24981863//27773535//22608085 chr13:53035093-53035094(+) m6A CDS//intron       24284625//24981863//27773535//22608085 chr13:53035100-53035101(+) m6A CDS//intron       24284625//24981863//27773535//22608085 chr13:53035205-53035206(+) m6A 5'UTR//CDS       24284625//24981863//27773535//22608085 chr13:53035215-53035216(+) m6A CDS       24284625//24981863//27773535//22608085 chr13:53035251-53035252(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035269-53035270(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035281-53035282(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035317-53035318(+) m6A CDS       24284625//24209618//24981863//26404942//27773535//22608085 chr13:53035336-53035337(+) m6A CDS       24284625//24209618//24981863//26404942//27773535//22608085 chr13:53035412-53035413(+) m6A CDS       24284625//24209618//24981863//26404942//27773535//22608085 chr13:53035428-53035429(+) m6A CDS       24284625//24209618//24981863//26404942//27773535//22608085 chr13:53035446-53035447(+) m6A CDS       24284625//24209618//24981863//26404942//27773535//22608085 chr13:53035476-53035477(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035536-53035537(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035578-53035579(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035608-53035609(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035622-53035623(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035703-53035704(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035754-53035755(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035764-53035765(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035780-53035781(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035824-53035825(+) m6A CDS       24284625//24209618//24981863//26404942//27773535//22608085 chr13:53035840-53035841(+) m6A CDS       24284625//24209618//24981863//26404942//27773535//22608085 chr13:53035861-53035862(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035894-53035895(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53035956-53035957(+) m6A CDS       24284625//24209618//24981863//26404942//27773535//22608085 chr13:53035992-53035993(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53036044-53036045(+) m6A CDS       24284625//24209618//24981863//27773535//22608085 chr13:53036584-53036585(+) m6A CDS       24981863//27773535//22608085 chr13:53036596-53036597(+) m6A CDS       24981863//27773535//22608085 chr13:53036615-53036616(+) m6A CDS       24981863//27773535 chr13:53036638-53036639(+) m6A CDS       24981863//27773535 chr13:53036664-53036665(+) m6A CDS       24981863//27773535 chr13:53036688-53036689(+) m6A CDS       24981863 chr13:53048051-53048052(+) m6A CDS//exon       24284625//24981863//27773535//22608085 chr13:53048069-53048070(+) m6A CDS//exon       24284625//24981863//27773535//22608085 chr13:53048113-53048114(+) m6A CDS//exon       24284625//24981863//27773535//22608085 chr13:53048121-53048122(+) m6A CDS//exon       24284625//24981863//27773535//22608085 chr13:53048148-53048149(+) m6A CDS//exon       24284625//24981863//27773535//22608085 chr13:53048169-53048170(+) m6A CDS//exon       24284625//24981863//27773535//22608085 chr13:53049057-53049058(+) m6A CDS//exon       24284625//24981863//22608085 chr13:53049126-53049127(+) m6A CDS//exon       24284625//24981863//22608085 chr13:53049182-53049183(+) m6A CDS//exon       24284625//24981863//22608085 chr13:53049207-53049208(+) m6A CDS//exon       24284625//24981863//22608085 chr13:53049714-53049715(+) Y 3'UTR       25219674

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	CKAP2	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Insoluble cytoplasm HCC cell line (HepG2)     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     - Ribosome HEK-293 cells     22199352 Ribosome-free cytosol Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: CKAP2

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.