Basic Information:

  VIRBase ID:  

VHID00507462

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          CNOT8:  NM_001301073     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	CNOT8
miRBase Accession/Entrez ID	MIMAT0001581	9337
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	CAF1//Caf1b//CALIF//hCAF1//POP2

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	CNOT8	chr5:154253873(+) A-I      intronic chr5:154253882(+) A-I      intronic chr5:154253908(+) A-I      intronic chr5:154253909(+) A-I      intronic chr5:154247721(+) A-I      intronic chr5:154246362(+) A-I      intronic chr5:154245848(+) A-I      intronic chr5:154241987(+) A-I      intronic chr5:154241903(+) A-I      intronic chr5:154241883(+) A-I      intronic chr5:154241457(+) A-I      intronic chr5:154241395(+) A-I      intronic chr5:154241359(+) A-I      intronic chr5:154241358(+) A-I      intronic chr5:154254331(+) A-I      intronic chr5:154253927(+) A-I      intronic chr5:154253993(+) A-I      intronic chr5:154254009(+) A-I      intronic chr5:154254042(+) A-I      intronic chr5:154254043(+) A-I      intronic chr5:154254051(+) A-I      intronic chr5:154254169(+) A-I      intronic chr5:154254198(+) A-I      intronic chr5:154254657(+) A-I      intronic chr5:154254219(+) A-I      intronic chr5:154254259(+) A-I      intronic chr5:154254280(+) A-I      intronic chr5:154254292(+) A-I      intronic chr5:154254295(+) A-I      intronic chr5:154254304(+) A-I      intronic chr5:154254317(+) A-I      intronic chr5:154247708(+) A-I      intronic chr5:154246366(+) A-I      intronic chr5:154246423(+) A-I      intronic chr5:154249205(+) A-I      intronic chr5:154249302(+) A-I      intronic chr5:154249310(+) A-I      intronic chr5:154249311(+) A-I      intronic chr5:154241339(+) A-I      intronic chr5:154246352(+) A-I      intronic chr5:154247517(+) A-I      intronic chr5:154249906(+) A-I      intronic chr5:154253875(+) A-I      intronic chr5:154249368(+) A-I      intronic chr5:154248034(+) A-I      intronic chr5:154246455(+) A-I      intronic chr5:154248022(+) A-I      intronic chr5:154243926(+) A-I      intronic chr5:154243102(+) A-I      intronic chr5:154246353(+) A-I      intronic chr5:154253976(+) A-I      intronic chr5:154254199(+) A-I      intronic chr5:154241845(+) A-I      intronic chr5:154241909(+) A-I      intronic chr5:154254223(+) A-I      intronic chr5:154241465(+) A-I      intronic chr5:154254616(+) A-I      intronic chr5:154248063(+) A-I      intronic chr5:154253980(+) A-I      intronic chr5:154244530(+) A-I      intronic chr5:154241440(+) A-I      intronic chr5:154245847(+) A-I      intronic chr5:154240051(+) A-I      intronic chr5:154254729(+) A-I      intronic chr5:154245786(+) A-I      intronic chr5:154241520(+) A-I      intronic chr5:154253881(+) A-I      intronic chr5:154243064(+) A-I      intronic chr5:154244375(+) A-I      intronic chr5:154243870(+) A-I      intronic chr5:154249444(+) A-I      intronic chr5:154254622(+) A-I      intronic chr5:154245861(+) A-I      intronic chr5:154241816(+) A-I      intronic chr5:154244494(+) A-I      intronic chr5:154246215(+) A-I      intronic chr5:154241443(+) A-I      intronic chr5:154249502(+) A-I      intronic chr5:154246388(+) A-I      intronic chr5:154250861(+) A-I      intronic chr5:154241416(+) A-I      intronic chr5:154241445(+) A-I      intronic chr5:154241919(+) A-I      intronic chr5:154254015(+) A-I      intronic chr5:154249904(+) A-I      intronic chr5:154245851(+) A-I      intronic chr5:154254355(+) A-I      intronic chr5:154250999(+) A-I      intronic chr5:154249737(+) A-I      intronic chr5:154243873(+) A-I      intronic chr5:154249570(+) A-I      intronic chr5:154253989(+) A-I      intronic chr5:154254739(+) A-I      intronic chr5:154241860(+) A-I      intronic chr5:154245761(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	CNOT8	chr5:154255685(+) A-I exon 3'UTR    19478186 chr5:154245861(+) A-I intron -    22327324 chr5:154243064(+) A-I intron -    21960545 chr5:154244359(+) A-I intron -    21960545 chr5:154245826(+) A-I intron -    21960545 chr5:154241278(+) A-G intron -    22484847 chr5:154241388(+) A-G intron -    22484847 chr5:154241443(+) A-G intron -    22484847 chr5:154241445(+) A-G intron -    22484847 chr5:154241465(+) A-G intron -    22484847 chr5:154241845(+) A-G intron -    22484847 chr5:154241860(+) A-G intron -    22484847 chr5:154241907(+) A-G intron -    22484847 chr5:154241909(+) A-G intron -    22484847 chr5:154241919(+) A-G intron -    22484847 chr5:154243064(+) A-G intron -    22484847 chr5:154243938(+) A-G intron -    22484847 chr5:154245761(+) A-G intron -    22484847 chr5:154245770(+) A-G intron -    22484847 chr5:154245786(+) A-G intron -    22484847 chr5:154245826(+) A-G intron -    22484847 chr5:154245847(+) A-G intron -    22484847 chr5:154245861(+) A-G intron -    22484847 chr5:154246209(+) A-G intron -    22484847 chr5:154246215(+) A-G intron -    22484847 chr5:154246230(+) C-T intron -    22484847 chr5:154246353(+) A-G intron -    22484847 chr5:154247621(+) C-T intron -    22484847 chr5:154247622(+) G-A intron -    22484847 chr5:154248804(+) T-C intron -    22484847 chr5:154253875(+) A-G intron -    22484847 chr5:154254015(+) A-G intron -    22484847 chr5:154254016(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	CNOT8	chr5:154237997-154237998(+) m6A 5'UTR//exon//intron       - chr5:154238149-154238150(+) m6A 5'UTR//intron       24981863 chr5:154252139-154252140(+) Am 3'UTR//CDS//exon       - chr5:154254862-154254863(+) m6A 3'UTR//CDS       24209618 chr5:154254943-154254944(+) m6A 3'UTR//CDS       24284625//24209618//25456834//24981863//22608085 chr5:154255129-154255130(+) m6A 3'UTR       24284625//24209618//24981863//22608085 chr5:154255147-154255148(+) m6A 3'UTR       24284625//24209618//24981863//22608085 chr5:154255169-154255170(+) m6A 3'UTR       24284625//24209618//24981863//22608085 chr5:154255300-154255301(+) m6A 3'UTR       24284625 chr5:154256135-154256136(+) m6A 3'UTR       27773535 chr5:154256147-154256148(+) m6A 3'UTR       26404942//27773535 chr5:154256153-154256154(+) m6A 3'UTR       26404942//27773535 chr5:154256180-154256181(+) m6A 3'UTR       26404942//27773535 chr5:154256235-154256236(+) m6A 3'UTR       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	CNOT8	Cytosol K562 cells     - Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleoplasm K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: CNOT8

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.