Basic Information:

  VIRBase ID:  

VHID00506600

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          MAPKAPK3:  NM_001243926     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	MAPKAPK3
miRBase Accession/Entrez ID	MIMAT0001581	7867
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	3PK//MAPKAP-K3//MAPKAP3//MAPKAPK-3//MDPT3//MK-3//MK3

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	MAPKAPK3	chr3:50673336(+) A-I      intronic chr3:50673335(+) A-I      intronic chr3:50673280(+) A-I      intronic chr3:50673281(+) A-I      intronic chr3:50673333(+) A-I      intronic chr3:50673328(+) A-I      intronic chr3:50673291(+) A-I      intronic chr3:50673028(+) A-I      intronic chr3:50673462(+) A-I      intronic chr3:50673318(+) A-I      intronic chr3:50673120(+) A-I      intronic chr3:50673276(+) A-I      intronic chr3:50658931(+) A-I      intronic chr3:50673412(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	MAPKAPK3	chr3:50673028(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	MAPKAPK3	chr3:50649076-50649077(+) m6A exon       24981863//27371828 chr3:50649113-50649114(+) m6A exon       24981863//27371828 chr3:50649142-50649143(+) m6A exon       24981863//27371828 chr3:50649530-50649531(+) m6A 5'UTR//exon       - chr3:50649801-50649802(+) m6A exon//intron       - chr3:50649809-50649810(+) m6A exon//intron       - chr3:50649862-50649863(+) m6A exon//intron       - chr3:50649888-50649889(+) m6A exon//intron       - chr3:50649954-50649955(+) m6A exon//intron       - chr3:50650018-50650019(+) m6A exon//intron       - chr3:50650077-50650078(+) m6A exon//intron       - chr3:50650104-50650105(+) m6A exon//intron       - chr3:50650123-50650124(+) m6A exon//intron       - chr3:50650331-50650332(+) m6A 5'UTR//exon       - chr3:50650338-50650339(+) m6A 5'UTR//exon       - chr3:50650351-50650352(+) m6A 5'UTR//exon       - chr3:50651230-50651231(+) m6A 5'UTR//exon       - chr3:50654920-50654921(+) m6A 5'UTR//intron       - chr3:50655008-50655009(+) m6A CDS       24981863 chr3:50655058-50655059(+) m6A CDS       - chr3:50685522-50685523(+) m6A 3'UTR       24284625//24981863//22608085 chr3:50685613-50685614(+) m6A 3'UTR       24284625//24981863//22608085 chr3:50685628-50685629(+) m6A 3'UTR       24284625//24981863//22608085 chr3:50685641-50685642(+) m6A 3'UTR       24284625//24981863//22608085 chr3:50686433-50686434(+) m6A 3'UTR       24284625 chr3:50686645-50686646(+) m6A 3'UTR       24284625 chr3:50686652-50686653(+) m6A 3'UTR       24284625

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	MAPKAPK3	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol K562 cells     - Exosome Blood     - Insoluble cytoplasm HCC cell line (HepG2)     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HeLa-S3 cells     - Ribosome HEK-293 cells     22199352 Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: MAPKAPK3

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.