Basic Information:

  VIRBase ID:  

VHID00506386

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          VEGFA:  NM_001171622     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	VEGFA
miRBase Accession/Entrez ID	MIMAT0001581	7422
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	MVCD1//VEGF//VPF

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	VEGFA	chr6:43738036-43738037(+) m6A 5'UTR//exon       24981863 chr6:43738048-43738049(+) m6A 5'UTR//exon       24981863 chr6:43738124-43738125(+) m6A 5'UTR//exon       24981863 chr6:43738139-43738140(+) m6A 5'UTR//exon       24284625 chr6:43738249-43738250(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960//22608085 chr6:43738450-43738451(+) m6A CDS//exon       24284625//24209618//25456834//24981863//27371828//27773536//22575960//22608085 chr6:43738458-43738459(+) m6A CDS//exon       24284625//24209618//25456834//24981863//27371828//27773536//22575960//22608085 chr6:43738509-43738510(+) m6A CDS//exon       24284625//24209618//25456834//24981863//27371828//27773536//22575960//22608085 chr6:43738601-43738602(+) m6A 5'UTR//CDS//exon       24284625//24209618//25456834//24981863//27371828//27773536//22575960//22608085 chr6:43738738-43738739(+) m1A 5'UTR//CDS//exon       26863196//26863410 chr6:43738825-43738826(+) m6A 5'UTR//CDS//exon       24284625//24209618//25456834//24981863//27371828//27773536//22575960//22608085 chr6:43738980-43738981(+) m6A 5'UTR//CDS//exon       24284625//24209618//25456834//24981863//27371828//27773536//22575960 chr6:43738987-43738988(+) m6A CDS//exon       24284625//24209618//25456834//24981863//27371828//27773536//22575960 chr6:43741786-43741787(+) m6A exon//intron       - chr6:43745461-43745462(+) m6A exon//intron       27773535 chr6:43745833-43745834(+) m6A exon//intron       - chr6:43745926-43745927(+) m6A exon//intron       - chr6:43746210-43746211(+) m6A CDS//exon//intron       24284625//24209618 chr6:43746477-43746478(+) m6A exon//intron       24981863 chr6:43746646-43746647(+) m6A CDS//exon//intron       24981863 chr6:43746762-43746763(+) m6A exon//intron       24981863 chr6:43747745-43747746(+) m6A exon//intron       - chr6:43748323-43748324(+) m6A exon//intron       - chr6:43749769-43749770(+) m6A CDS//exon//intron       24284625 chr6:43749775-43749776(+) m6A CDS//exon//intron       24284625 chr6:43752338-43752339(+) m6A 3'UTR//exon//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr6:43752360-43752361(+) m6A 3'UTR//CDS//exon       24284625//24209618//25456834//24981863//26404942//27773535//27371828//22575960//22608085 chr6:43752386-43752387(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//27371828//22575960//22608085 chr6:43752423-43752424(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//27371828//22575960//22608085 chr6:43752441-43752442(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr6:43752463-43752464(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr6:43752498-43752499(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr6:43752632-43752633(+) m6A 3'UTR//exon       24284625//24209618//24981863//22608085 chr6:43752790-43752791(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr6:43752852-43752853(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr6:43752961-43752962(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr6:43752971-43752972(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr6:43753013-43753014(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr6:43753021-43753022(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr6:43753022-43753023(+) Cm 3'UTR//exon       - chr6:43753046-43753047(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr6:43753082-43753083(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr6:43753211-43753212(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr6:43753243-43753244(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr6:43753288-43753289(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//22575960//22608085 chr6:43753339-43753340(+) m6A 3'UTR//exon       24284625//24209618//24981863//22575960//22608085 chr6:43753362-43753363(+) m6A 3'UTR//exon       24284625//24209618//24981863//22575960//22608085 chr6:43753387-43753388(+) m6A 3'UTR//exon       24284625//24209618//24981863//22575960//22608085 chr6:43753464-43753465(+) m6A 3'UTR//exon       24284625//24209618//22575960 chr6:43753562-43753563(+) m6A 3'UTR//exon       24284625

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	VEGFA	Chromatin K562 cells     - Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539 Endoplasmic reticulum Human fibrosarcoma cells (HT1080)     25753659 Exosome Blood     - Insoluble cytoplasm HCC cell line (HepG2)     - Membrane K562 cells     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus Colon cancer cells     24393600 Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     - Ribosome HeLa cells     25063809

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: VEGFA

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.