Basic Information:

  VIRBase ID:  

VHID00505063

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          PRKAA1:  NM_001355037     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	PRKAA1
miRBase Accession/Entrez ID	MIMAT0001581	5562
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	AMPK//AMPKa1

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	PRKAA1	chr5:40770799(-) A-I      intronic chr5:40777930(-) A-I      intronic chr5:40785479(-) A-I      intronic chr5:40785524(-) A-I      intronic chr5:40789091(-) A-I      intronic chr5:40789096(-) A-I      intronic chr5:40789121(-) A-I      intronic chr5:40789147(-) A-I      intronic chr5:40789256(-) A-I      intronic chr5:40789307(-) A-I      intronic chr5:40777844(-) A-I      intronic chr5:40770805(-) A-I      intronic chr5:40770541(-) A-I      intronic chr5:40770485(-) A-I      intronic chr5:40770373(-) A-I      intronic chr5:40789230(-) A-I      intronic chr5:40770360(-) A-I      intronic chr5:40789141(-) A-I      intronic chr5:40779030(-) A-I      intronic chr5:40762291(-) A-I      3'UTR chr5:40762262(-) A-I      3'UTR chr5:40762190(-) A-I      3'UTR chr5:40762141(-) A-I      3'UTR chr5:40762181(-) A-I      3'UTR chr5:40789056(-) A-I      intronic chr5:40770492(-) A-I      intronic chr5:40777394(-) A-I      intronic chr5:40788781(-) A-I      intronic chr5:40785615(-) A-I      intronic chr5:40778230(-) A-I      intronic chr5:40771493(-) A-I      intronic chr5:40764153(-) A-I      ncRNA chr5:40762119(-) A-I      3'UTR chr5:40770561(-) A-I      intronic chr5:40770822(-) A-I      intronic chr5:40767245(-) A-I      intronic chr5:40772868(-) A-I      intronic chr5:40770832(-) A-I      intronic chr5:40772900(-) A-I      intronic chr5:40785624(-) A-I      intronic chr5:40777152(-) A-I      intronic chr5:40779125(-) A-I      intronic chr5:40786945(-) A-I      intronic chr5:40767246(-) A-I      intronic chr5:40770321(-) A-I      intronic chr5:40779156(-) A-I      intronic chr5:40772978(-) A-I      intronic chr5:40785610(-) A-I      intronic chr5:40770308(-) A-I      intronic chr5:40770347(-) A-I      intronic chr5:40766907(-) A-I      intronic chr5:40777791(-) A-I      intronic chr5:40779096(-) A-I      intronic chr5:40779097(-) A-I      intronic chr5:40766917(-) A-I      intronic chr5:40764149(-) A-I      ncRNA

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	PRKAA1	chr5:40762119(-) A-G exon 3'UTR    22484847 chr5:40770720(-) T-C intron -    22484847 chr5:40777394(-) A-G intron -    22484847 chr5:40785610(-) A-G intron -    22484847 chr5:40789299(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	PRKAA1	chr5:40760600-40760601(-) m6A 3'UTR       24981863 chr5:40760879-40760880(-) m6A 3'UTR       24981863//26404942//22608085 chr5:40761318-40761319(-) m6A 3'UTR       26404942 chr5:40761998-40761999(-) m6A 3'UTR       27773535 chr5:40762037-40762038(-) m6A 3'UTR       27773535 chr5:40762871-40762872(-) m6A 3'UTR       24981863//27773535//22608085 chr5:40762878-40762879(-) m6A 3'UTR       24981863//27773535//22608085 chr5:40762945-40762946(-) m6A CDS       24284625//24981863//27773535//22608085 chr5:40763083-40763084(-) Gm CDS       - chr5:40763097-40763098(-) m6A CDS       24284625//24981863//27773535//27371828//22608085 chr5:40764582-40764583(-) m6A exon//intron       24284625//24981863//27773535 chr5:40764596-40764597(-) m6A exon//intron       24284625//24981863//26404942//27773535//22608085 chr5:40764645-40764646(-) m6A CDS//exon       24284625//24981863//26404942//27773535//22608085 chr5:40764734-40764735(-) m6A CDS//exon       24284625 chr5:40764874-40764875(-) m6A CDS//exon       24284625 chr5:40764967-40764968(-) m6A CDS//exon       24284625 chr5:40765017-40765018(-) m6A CDS//exon       24284625//22608085 chr5:40777680-40777681(-) m6A CDS//exon       24981863//27371828//22575960 chr5:40798240-40798241(-) m6A CDS//exon       24284625//25456834//24981863//27371828//22575960 chr5:40798282-40798283(-) m6A CDS//exon       24284625//25456834//24981863//27371828

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	PRKAA1	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HeLa-S3 cells     - Exosome Blood     - Nucleoplasm K562 cells     - Nucleus Colon cancer cells     24393600 Nucleus HCC cell line (HepG2)|K562 cells     -

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: PRKAA1

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.