Basic Information:

  VIRBase ID:  

VHID00501991

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          CAPN2:  NM_001748     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	CAPN2
miRBase Accession/Entrez ID	MIMAT0001581	824
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	CANP2//CANPL2//CANPml//mCANP

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	CAPN2	chr1:223950652(+) A-I      intronic chr1:223960904(+) A-I      intronic chr1:223950664(+) A-I      intronic chr1:223933217(+) A-I      intronic chr1:223950637(+) A-I      intronic chr1:223950626(+) A-I      intronic chr1:223910761(+) A-I      intronic chr1:223910901(+) A-I      intronic chr1:223956865(+) A-I      intronic chr1:223956962(+) A-I      intronic chr1:223954452(+) A-I      intronic chr1:223910922(+) A-I      intronic chr1:223957006(+) A-I      intronic chr1:223957032(+) A-I      intronic chr1:223957097(+) A-I      intronic chr1:223950628(+) A-I      intronic chr1:223923754(+) A-I      intronic chr1:223907162(+) A-I      intronic chr1:223907147(+) A-I      intronic chr1:223923582(+) A-I      intronic chr1:223954555(+) A-I      intronic chr1:223910844(+) A-I      intronic chr1:223910810(+) A-I      intronic chr1:223907094(+) A-I      intronic chr1:223910213(+) A-I      intronic chr1:223923682(+) A-I      intronic chr1:223922958(+) A-I      intronic chr1:223907062(+) A-I      intronic chr1:223910139(+) A-I      intronic chr1:223923691(+) A-I      intronic chr1:223924473(+) A-I      intronic chr1:223933258(+) A-I      intronic chr1:223929963(+) A-I      intronic chr1:223910936(+) A-I      intronic chr1:223907120(+) A-I      intronic chr1:223910841(+) A-I      intronic chr1:223923551(+) A-I      intronic chr1:223954541(+) A-I      intronic chr1:223957003(+) A-I      intronic chr1:223910885(+) A-I      intronic chr1:223930485(+) A-I      intronic chr1:223912416(+) A-I      intronic chr1:223907121(+) A-I      intronic chr1:223907126(+) A-I      intronic chr1:223954542(+) A-I      intronic chr1:223912446(+) A-I      intronic chr1:223952250(+) A-I      intronic chr1:223923559(+) A-I      intronic chr1:223923065(+) A-I      intronic chr1:223923070(+) A-I      intronic chr1:223922980(+) A-I      intronic chr1:223923110(+) A-I      intronic chr1:223923119(+) A-I      intronic chr1:223923059(+) A-I      intronic chr1:223923562(+) A-I      intronic chr1:223912554(+) A-I      intronic chr1:223923077(+) A-I      intronic chr1:223923561(+) A-I      intronic chr1:223956949(+) A-I      intronic chr1:223954409(+) A-I      intronic chr1:223923044(+) A-I      intronic chr1:223923029(+) A-I      intronic chr1:223950799(+) A-I      intronic chr1:223961439(+) A-I      intronic chr1:223957168(+) A-I      intronic chr1:223908097(+) A-I      intronic chr1:223961103(+) A-I      intronic chr1:223954404(+) A-I      intronic chr1:223910013(+) A-I      intronic chr1:223923621(+) A-I      intronic chr1:223926141(+) A-I      intronic chr1:223929786(+) A-I      intronic chr1:223910931(+) A-I      intronic chr1:223908130(+) A-I      intronic chr1:223897313(+) A-I      intronic chr1:223956952(+) A-I      intronic chr1:223956956(+) A-I      intronic chr1:223954403(+) A-I      intronic chr1:223933218(+) A-I      intronic chr1:223951567(+) A-I      intronic chr1:223910190(+) A-I      intronic chr1:223912683(+) A-I      intronic chr1:223910064(+) A-I      intronic chr1:223912490(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	CAPN2	chr1:223922980(+) A-I intron -    22028664 chr1:223923059(+) A-I intron -    22028664 chr1:223923065(+) A-I intron -    22028664 chr1:223923070(+) A-I intron -    22028664 chr1:223923077(+) A-I intron -    22028664 chr1:223923110(+) A-I intron -    22028664 chr1:223923119(+) A-I intron -    22028664 chr1:223923123(+) A-I intron -    22028664 chr1:223954404(+) A-I intron -    21960545 chr1:223910761(+) A-I intron -    21960545 chr1:223930413(+) A-I intron -    21960545 chr1:223962879(+) G-A intron -    21725310 chr1:223908097(+) A-G intron -    22484847 chr1:223908130(+) A-G intron -    22484847 chr1:223912554(+) A-G intron -    22484847 chr1:223923559(+) A-G intron -    22484847 chr1:223923561(+) A-G intron -    22484847 chr1:223923562(+) A-G intron -    22484847 chr1:223923621(+) A-G intron -    22484847 chr1:223954403(+) A-G intron -    22484847 chr1:223954404(+) A-G intron -    22484847 chr1:223956956(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	CAPN2	chr1:223889300-223889301(+) m6A 5'UTR       24981863 chr1:223889339-223889340(+) m6A 5'UTR       24981863 chr1:223889387-223889388(+) m6A 5'UTR       24981863 chr1:223900334-223900335(+) m6A 5'UTR//intron       24284625//24981863//22575960 chr1:223900373-223900374(+) m6A CDS//intron       24284625//26404942//22575960 chr1:223900433-223900434(+) m6A CDS//intron       24284625//26404942//22575960 chr1:223900484-223900485(+) m6A CDS//intron       24284625//22575960 chr1:223900549-223900550(+) m6A CDS//intron       24284625//22575960 chr1:223905512-223905513(+) m6A CDS       24284625//22575960 chr1:223931876-223931877(+) m6A CDS       26404942//22575960 chr1:223931894-223931895(+) m6A CDS       26404942//22575960 chr1:223934754-223934755(+) m6A CDS       26404942 chr1:223936756-223936757(+) m6A CDS//exon       26404942//22575960 chr1:223937129-223937130(+) m6A exon//intron       22575960 chr1:223938617-223938618(+) m6A CDS       24981863//22575960 chr1:223938657-223938658(+) m6A CDS       24981863//26404942//22575960 chr1:223939712-223939713(+) m6A CDS       24981863//26404942//22575960 chr1:223939721-223939722(+) m6A CDS//exon       24981863//26404942//22575960 chr1:223940559-223940560(+) m6A CDS//exon       24981863//26404942//22575960 chr1:223940593-223940594(+) m6A CDS//exon       24981863//26404942//22575960 chr1:223940613-223940614(+) m6A CDS//exon       24284625//24981863//26404942//22575960 chr1:223940649-223940650(+) m6A CDS//exon       24284625//24981863//26404942//22575960 chr1:223943196-223943197(+) m6A CDS//exon       24284625//25456834//24981863//26404942//22575960 chr1:223943319-223943320(+) m6A CDS//exon       24284625//25456834//24981863//26404942//22575960 chr1:223945138-223945139(+) m6A exon//intron       24284625 chr1:223945366-223945367(+) m6A exon//intron       25456834//24981863 chr1:223945412-223945413(+) m6A exon//intron       25456834//24981863 chr1:223945433-223945434(+) m6A exon//intron       25456834//24981863 chr1:223945442-223945443(+) m6A exon//intron       25456834//24981863 chr1:223945455-223945456(+) m6A exon//intron       25456834//24981863 chr1:223945477-223945478(+) m6A exon//intron       25456834//24981863 chr1:223945483-223945484(+) m6A exon//intron       25456834//24981863 chr1:223945511-223945512(+) m6A exon//intron       25456834//24981863 chr1:223946983-223946984(+) m6A CDS//exon       26404942 chr1:223947005-223947006(+) m6A CDS//exon       26404942 chr1:223947041-223947042(+) m6A CDS//exon       26404942 chr1:223947123-223947124(+) m6A CDS//exon       22575960 chr1:223949928-223949929(+) m6A CDS//exon       - chr1:223951881-223951882(+) m6A CDS//exon       22575960 chr1:223954103-223954104(+) m6A CDS//exon       22575960 chr1:223957909-223957910(+) m6A exon//intron       24284625//24981863 chr1:223957915-223957916(+) m6A exon//intron       24284625//24981863 chr1:223957921-223957922(+) m6A exon//intron       24284625//24981863 chr1:223957964-223957965(+) m6A exon//intron       24981863 chr1:223957976-223957977(+) m6A exon//intron       24981863 chr1:223958012-223958013(+) m6A exon//intron       24981863 chr1:223958199-223958200(+) Gm CDS//exon       - chr1:223958872-223958873(+) m6A exon//intron       - chr1:223960896-223960897(+) m6A exon//intron       24284625 chr1:223962582-223962583(+) m6A 3'UTR//exon       24284625//24981863//26404942//22608085 chr1:223962610-223962611(+) m6A 3'UTR//exon       24284625//24981863//26404942//22608085 chr1:223962644-223962645(+) m6A 3'UTR//exon       24284625//24981863//22608085 chr1:223962661-223962662(+) m6A 3'UTR//exon       24284625//24981863//22608085 chr1:223962918-223962919(+) m6A 3'UTR//exon       26404942 chr1:223962939-223962940(+) m6A 3'UTR//exon       26404942 chr1:223963008-223963009(+) m6A 3'UTR//exon       26404942 chr1:223963050-223963051(+) m6A 3'UTR//exon       26404942 chr1:223963106-223963107(+) m6A 3'UTR//exon       26404942 chr1:223963130-223963131(+) m6A 3'UTR//exon       26404942 chr1:223963137-223963138(+) m6A 3'UTR//exon       26404942 chr1:223963233-223963234(+) m6A 3'UTR//exon       24284625//26404942//27773535 chr1:223963263-223963264(+) m6A 3'UTR//exon       24284625//24981863//26404942//27773535 chr1:223963277-223963278(+) m6A 3'UTR//exon       24284625//24981863//26404942//27773535 chr1:223963297-223963298(+) m6A 3'UTR//exon       24284625//24981863//26404942//27773535 chr1:223963314-223963315(+) m6A 3'UTR//exon       24284625//24981863//26404942//27773535 chr1:223963341-223963342(+) m6A 3'UTR//exon       24284625//24981863//26404942//27773535 chr1:223963350-223963351(+) m6A 3'UTR//exon       24284625//24981863//26404942//27773535 chr1:223963390-223963391(+) m6A 3'UTR//exon       24981863//27773535 chr1:223963418-223963419(+) m6A 3'UTR//exon       24981863//27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	CAPN2	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HeLa-S3 cells     - Exosome Blood     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     - Ribosome HEK-293 cells     22199352 Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: CAPN2

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.