Basic Information:

  VIRBase ID:  

VHID00501682

  Virus:  

Human herpesvirus 5 strain AD169

  Host:  

Homo sapiens

  Confidence Score:  

0.7311

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US25-1-5p:  MIMAT0001581          ARAF:  NM_001256197     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US25-1-5p	ARAF
miRBase Accession/Entrez ID	MIMAT0001581	369
Organism	Human herpesvirus 5 strain AD169	Homo sapiens
Category	miRNA	mRNA
Alias	-	A-RAF//ARAF1//PKS2//RAFA1

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	ARAF	chrX:47420585-47420586(+) m6A 5'UTR       24284625//25456834//24981863 chrX:47420598-47420599(+) m6A 5'UTR       24284625//25456834//24981863 chrX:47422664-47422665(+) m6A CDS//exon       22575960 chrX:47422697-47422698(+) m6A CDS//exon       22575960 chrX:47424218-47424219(+) m6A CDS//exon       22575960 chrX:47424604-47424605(+) m6A exon//intron       24284625 chrX:47424689-47424690(+) m6A CDS       24284625 chrX:47424709-47424710(+) m6A CDS       24284625 chrX:47424808-47424809(+) m6A 3'UTR//intron       24284625 chrX:47424846-47424847(+) m6A 3'UTR//intron       24284625 chrX:47425321-47425322(+) m6A 3'UTR//intron       24284625//24209618//24981863 chrX:47426162-47426163(+) m6A CDS       24284625//24981863 chrX:47426413-47426414(+) m6A CDS       24284625//22575960 chrX:47426632-47426633(+) m6A CDS       24284625//22575960 chrX:47426647-47426648(+) m6A CDS       24284625//22575960 chrX:47428276-47428277(+) m6A CDS       22575960 chrX:47428402-47428403(+) m6A CDS       22575960 chrX:47428872-47428873(+) m6A exon//intron       22575960 chrX:47429578-47429579(+) m6A exon//intron       - chrX:47429648-47429649(+) m6A exon//intron       - chrX:47430313-47430314(+) m6A CDS//exon       24284625 chrX:47430783-47430784(+) m6A CDS//exon       24284625//22575960 chrX:47431027-47431028(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//22575960 chrX:47431040-47431041(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//22575960//22608085 chrX:47431157-47431158(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chrX:47431264-47431265(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//27773535//22575960//22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	ARAF	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HeLa-S3 cells|K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US25-1-5p
Interactor2: ARAF

Evidence Support:

Strong-Evidence	RISC-IP
Support Database	ViRBase

References:

PMID	20585629	Target region	5'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	HEK293 cells	Interactor2 expression	None
Description	Here, we identify cellular transcript targets of one of the most highly expressed HCMV encoded miRNAs, miR-US25-1, using a recently developed biochemical approach called RISC immunoprecipitation. Strikingly, the majority of identified transcripts contained miR-US25-1 target sites within the 5'UTR rather than the 3'UTR.